On the field anhydrobiotic ability of Pratylenchis thornei and Merlinius brevidens

La présente étude du comportement anhydrobiotique de #Pratylenchus thornei et de #Merlinius brevidens a été réalisée dans un champ non irrigué de blé cv. Gallareta, variété considérée comme non-hôte ou mauvais hôte pour ces nématodes. #P. thornei paraît plus dépendant de l'humidité du sol que #M. brevidens en ce qui concerne sa récupération après 15 h de migration (réhydratation, réactivation et migration). #M. brevidens sort plus facilement de son état anhydrobiotique. Les schémas d'émergence sont semblables pour les deux nématodes qu'ils soient actifs ou récupérés après une anhydrobiose prononcée. Ces schémas sont par contre différents s'il s'agit d'un stade précoce d'anhydrobiose (anhydrobiose modérée). Les deux nématodes font montre, en relation avec la profondeur du sol, d'une inertie à entrer en anhydrobiose qui paraît dépendre des processus naturels de dessiccation du sol du champ. Cette inertie semble plus prononcée chez #M. brevidens. (Résumé d'auteur

Étude de la biodisponibilité d'anthocyanosides issus de fruits rouges

Numéro national de thèse : 2005CLF1PP04 Diplôme : Dr. d'Universit

Blackberry anthocyanins are mainly recovered from urine as methylated and glucuronidated conjugates in humans

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699International audienceThe consumption of anthocyanins has been shown to prevent certain chronic diseases. However, anthocyanin metabolism has not yet been fully elucidated. The aim of this study was to evaluate anthocyanin urinary excretion in humans receiving a meal containing blackberries and to identify possible metabolites in urine. Five healthy volunteers were fed 200 g of blackberries (960 mumol of anthocyanins). Urine samples were collected and rapidly treated by solid-phase extraction. Anthocyanin metabolites were identified and quantified by HPLC-ESI-MS-MS and HPLC with UV-vis detection, respectively. In addition to native cyanidin 3-glucoside, several other anthocyanin metabolites were identified in the urine: methylated glycosides, glucuronides of anthocyanidins and anthocyanins, a sulfoconjugate of cyanidin, and anthocyanidins. Total urinary excretion of blackberry anthocyanin metabolites was 0.160 +/- 0.020% (n = 5) of the amount of anthocyanins ingested. Monoglucuronides of anthocyanidins represented >60% of this excretion. Urinary excretion of anthocyanins was maximal between 2 and 4 h after the meal, but continued during the 24 h of the experiment. This study highlighted the influence of aglycon structure on anthocyanin urinary excretion. It demonstrated that anthocyanins are not only methylated but also glucuroconjugated and sulfoconjugated in humans and that the main metabolites of blackberry anthocyanins in human urine were anthocyanidin monoglucuronides

Anthocyanin metabolism in rats and their distribution to digestive area, kidney, and brain

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699International audienceAnthocyanins are present in human diet due to their wide occurrence in fruits and beverages. They possess antioxidant activities and could be involved in several health effects. The aim of this study was to investigate anthocyanin metabolism and distribution in the digestive area organs (stomach, jejunum and liver) and kidney, as well as a target tissue (brain) in rats fed with a blackberry (Rubus fruticosus L.) anthocyanin-enriched diet for 15 days. Identification and quantification of anthocyanin metabolites was carried out by HPLC-ESI-MS-MS and HPLC-DAD, respectively. The stomach exhibited only native blackberry anthocyanins (cyanidin 3-O-glucoside and cyanidin 3-O-pentose), while in other organs (jejunum, liver, and kidney) native and methylated anthocyanins as well as conjugated anthocyanidins (cyanidin and peonidin monoglucuronides) were identified. Proportions of anthocyanin derivatives differed according to the organ considered, with the liver presenting the highest proportion of methylated forms. Jejunum and plasma also contained aglycone forms. In the brain, total anthocyanin content (blackberry anthocyanins and peonidin 3-O-glucoside) reached 0.25 ± 0.05 nmol/g of tissue (n = 6). The urinary excretion of total anthocyanins was low (0.19 ± 0.02% of the ingested amount). Thus, organs of the digestive area indicated a metabolic pathway of anthocyanins with enzymatic conversions (methylation and/or glucurono-conjugation). Moreover, following consumption of an anthocyanin-rich diet, anthocyanins enter the brain