教師の職業ストレッサーにおける職場環境の要因と職務自体の要因がバーンアウトに与える影響の検討 ―職場環境要因が及ぼす緩衝効果（交互作用的効果）を中心に―

本研究の目的は教師の職業ストレッサーの中の「職場環境の要因」がストレッサー・ストレス反応過程である「職務自体の要因」・「バーンアウト」過程および「個人的ストレッサー」・「バーンアウト」過程にどのような緩衝効果を与えるかを検討することである。岡山県の小中学校教師710名を分析の対象とし2001年に調査を行った。まず、「職場環境の要因」4因子と「職務自体の要因」2因子の積計8変数と「職場環境の要因」4因子と「個人的要因」2因子の積計8変数の計16変数を独立変数とし、「バーンアウト」3因子を従属変数とする階層的重回帰分析を行った。その結果、やなどが緩衝効果を持つストレス予防に有効な変数であることが明らかにされた

Japanese Teacher's Similar Type of Job Stressors

本研究の目的は教師のメンタルヘルス促進の実践のために、高い「バーンアウト」を生じることが予測できる教師の職業ストレッサーの類型化をこころみることである。岡山県の小・中学校教師710名を対象に調査を行った。まず、「職業環境の要因」(28項目)と「職務事態の要因」(16項目)、「個人的要因」(5項目)からなる3種類の教師の職業ストレッサーの3要因の平均得点を代表値とし、それをもとに小学校・中学校教師それぞれにおいてクラスター分析を実施した。その結果、小学校は3つ、中学校は4つのクラスターが示された。次いで、各クラスターごとに「バーンアウト」の3要因の平均得点の比較を一元配置の分散分析で検討した。その結果、小学校のクラスターは「バーンアウト」の高さの一貫した差が確認できない一方で、中学校は特定のクラスターの順番にあわせてほぼ「バーンアウト」が悪化していく傾向が確認された。以上の結果を基に「個人的要因」のストレッサーとしての「バーンアウト」への影響過程の複雑さと今後の実践上の課題を議論した

Histological Observation of the Development of Follicles and Follicular Atresia in Immature Rat Ovaries

To clarify the development of follicular growth and atresia in the immature ovary, rats. ovaries and blood were removed at fixed points during the period from 0 to 35 days after birth (Day 0 to Day 35). The ovaries were immunohistochemically examined, and blood concentrations of serum follicle-stimulating hormone (FSH) and estrogen (E) were measured. We investigated how time-course changes in follicular cell proliferation, estrogen receptor β (ERβ), apoptosis, and FSH and E concentrations are connected with follicular growth and atresia. Apoptosis was found in the ova from Day 0 to Day 3. On Day 15, apoptosis occurred in some granulosa cell nuclei in some follicles, but BrdU uptake and the presence of cyclin D2 and ER β could be observed in other granulosa cells. From Day 17, apoptosis increased in the follicular granulosa cells, and BrdU uptake and the presence of cyclin D2 and ERβ were decreased. Follicular atresia continued, reaching a peak on Day 30. Serum FSH and E concentrations increased until Day 15, then markedly decreased after Day 17. The mechanism of apoptosis in the ova from Day 0 to 3 has not been clarified. However, the onset of follicular atresia was caused by apoptotic degeneration from Days 15 to 17. These results showed that the oocytes were selected by apoptosis at 2 points in the time-course of the maturation of the ovary

Apply of Textmining Method to Study the Roles in Improving the Health by Lactoferrin, a Multi-Functional Milk Protein

Lactoferrin is a metal-binding glycoprotein found in milk, blood and other exocrine secretions. This is a multi-functional protein that exhibits many activities such as: anti-microbial, anti-viral, immunomodulatory, anti-inflammatory, anti-tumor, anti-metastatic, cell growth-promoting, and anti-oxidant activities, as well as regulation of granulopoiesis and iron absorption, etc. To date, a number of academic reports concerning the biological activities of lactoferrin have been published and are easily accessible through public databases. In order to overcome the information overload associated with lactoferrin information, we have applied the text mining method to the accumulated lactoferrin literature. To this end, we used the information extraction system GENPAC (provided by Nalapro Technologies Inc., Tokyo), which uses natural language processing and text mining technology. Using GENPAC, text extraction was carried out on literature containing the term “lactoferrin” and any of keywords concerning health conditions or diseases from PubMed. Subsequently, network mappings of the information obtained were produced using Cytoscape. We will exhibit that such textmining method and information visualization analysis is useful in studying novel relationships among a multitude of lactoferrin functions and mechanisms to improve our health

Crystal structures of the RNA triphosphatase from Trypanosoma cruzi provide insights into how it recognizes the 5\u27 end of the RNA substrate

RNA triphosphatase catalyzes the first step in mRNA cap formation, hydrolysis of the terminal phosphate from the nascent mRNA transcript. The RNA triphosphatase from the protozoan parasite Trypanosoma cruzi, TcCet1, belongs to the family of triphosphate tunnel metalloenzymes (TTMs). TcCet1 is a promising antiprotozoal drug target because the mechanism and structure of the protozoan RNA triphosphatases are completely different from those of the RNA triphosphatases found in mammalian and arthropod hosts. Here, we report several crystal structures of the catalytically active form of TcCet1 complexed with a divalent cation and an inorganic tripolyphosphate in the active-site tunnel at 2.20-2.51 Å resolutions. The structures revealed that the overall structure, architecture of the tunnel, and arrangement of the metal-binding site in TcCet1 are similar to those in other TTM proteins. On the basis of the position of three sulfate ions that co-crystallized on the positively charged surface of the protein, and results obtained from mutational analysis, we identified an RNA-binding site in TcCet1. We conclude that the 5\u27 end of the triphosphate RNA substrate enters the active-site tunnel directionally. The structural information reported here provides valuable insight into designing inhibitors that could specifically block the entry of the triphosphate RNA substrate into the TTM-type RNA triphosphatases of T. cruzi and related pathogens

Performance of a newly developed SDCCD for X-ray use

A Scintillator Deposited CCD (SDCCD) is a wide-band X-ray detector consisting of a CCD and a scintillator directly attached to each other. We assembled the newly developed SDCCD that the scintillator CsI(Tl) is below the fully depleted CCD. The incident X-rays enter the CCD depletion layer first. Then, X-rays passing through the depletion layer are absorbed in the CsI(Tl). The contact surface of the CCD is a back-illuminated side so that we can have good light collection efficiency. In our experimental setup, we confirmed good performance of our SDCCD detecting many emission lines up to 88\,keV that comes from $^{109}$Cd.Comment: 4 pages, 6 figures, accepted publication for Nucl. Instr. and Meth. (2010

S100A9は、骨細胞様細胞においてMAPKsおよびSTAT3シグナル伝達経路を介してIL-6およびRANKLの発現を増加させる

Objective: Calprotectin is hetero-complex of S100A8 and S100A9 and mainly secreted from neutrophils, monocytes and chondrocytes in inflammatory condition. Calprotectin binds to RAGE and TLR4, and induces the expression of pro-inflammatory chemokines and cytokines in various cells. Periodontitis is chronic inflammatory disease to lead gingival inflammation and alveolar bone resorption. Calprotectin levels in gingival crevicular fluid of periodontitis patients are higher than healthy patients. In the present study, the effects of S100A8 and S100A9 on the expressions of pro-inflammatory cytokines and bone metabolism related factor in mouse osteocyte like cells (MLO-Y4-A2) were investigated. Design: MLO-Y4-A2 cells were treated with S100A8 and S100A9, and the expressions of RAGE, TLR4, RANKL and several inflammatory cytokines were analyzed by PCR and Western blotting or ELISA methods. To investigate the intracellular signaling pathways, phosphorylation of MAPK and STAT3 was determined by Western blotting, and chemical specific inhibitors and siRNAs were used. Results: Expressions of IL-6 and RANKL were increased by treatment with S100A9 but not S100A8. However, both S100A8 and S100A9 did not changed expression of IL-1β, IL-8 and TNF-α. Although RAGE and TLR4 expressions were not up-regulated by S100A9 treatment, transfection of siRNA for RAGE and TLR4 significantly decreased IL-6 and RANKL expressions. In addition, S100A9 activated p38, ERK and STAT3 signaling pathways, and inhibitors for these factors significantly decreased S100A9 induced IL-6 and RANKL expressions. Conclusions: These results indicated that S100A9 induces IL-6 and RANKL production via engagement with RAGE and TLR4 signalings in osteocytes and suggested that S100A9 may play important roles in the periodontal alveolar bone destruction

Demonstration of a robust magnonic spin wave interferometer

Magnonics is an emerging field dealing with ultralow power consumption logic circuits, in which the flow of spin waves, rather than electric charges, transmits and processes information. Waves, including spin waves, excel at encoding information via their phase using interference. This enables a number of inputs to be processed in one device, which offers the promise of multi-input multi-output logic gates. To realize such an integrated device, it is essential to demonstrate spin wave interferometers using spatially isotropic spin waves with high operational stability. However, spin wave reflection at the waveguide edge has previously limited the stability of interfering waves, precluding the use of isotropic spin waves, i.e., forward volume waves. Here, a spin wave absorber is demonstrated comprising a yttrium iron garnet waveguide partially covered by gold. This device is shown experimentally to be a robust spin wave interferometer using the forward volume mode, with a large ON/OFF isolation value of 13.7 dB even in magnetic fields over 30 Oe

Quantitative assessment of coronary stenosis by harmonic power Doppler with a simple pulsing sequence and vasodilator stress in patients

AbstractObjectivesWe examined whether myocardial contrast echocardiography (MCE) with harmonic power Doppler (HPD) employing a simple ultrasound pulsing sequence enables estimation of the severity of coronary artery stenosis in patients.BackgroundContrast intensity (CI) during MCE with intravenous microbubble infusion is dependent on the myocardial blood flow velocity (MBFV) and pulsing interval (PI).MethodsBased on an in vitro experiment, we devised the MBFV index calculated as the reciprocal of the magnitude of CI decay produced by abrupt PI shortening during intermittent imaging. In 68 coronary artery territories from 49 patients, myocardial HPD images were acquired during intravenous infusion of Levovist, while the long PI with 1:10 electrocardiographic gating was shortened to 1:1, both at baseline and during adenosine triphosphate infusion. The MBFV index in each coronary territory and MBFV reserve as the ratio between hyperemia and baseline were compared with the severity of corresponding coronary artery stenosis assessed by quantitative coronary angiography (QCA) or by pressure guide wire as the fractional flow reserve (FFR).ResultsBoth the MCE-derived MBFV index during hyperemia and MBFV reserve exhibited significant negative correlations with the QCA-derived stenosis severity (r = −0.56 and r = −0.64, respectively). The MBFV reserve positively correlated with FFR (r = 0.89). By combining the cutoff values of the MBFV index during hyperemia and MBFV reserve, ≥75% of stenoses defined by QCA were determined, with a sensitivity of 77.3%, specificity of 93.4%, and accuracy of 88.3%.ConclusionsShortening of PI during intravenous MCE with intermittent HPD imaging under vasodilator stress enables assessment of coronary artery stenoses in patients