Microwave and millimeter wave spectroscopy in the slightly hole-doped ladders of Sr14_{14}Cu24_{24}O41_{41}

We have measured the temperature- and frequency dependence of the microwave and millimeter wave conductivity $\sigma_1(T,\omega)$ along both the ladder (c-axis) and the leg (a-axis) directions in Sr$_{14}$Cu$_{24}$O$_{41}$. Below a temperature $T^*$($\sim$170 K), we observed a stronger frequency dependence in $\sigma_1^c(T,\omega)$ than that in $\sigma_1^a(T,\omega)$, forming a small resonance peak developed between 30 GHz and 100 GHz. We also observed nonlinear dc conduction along the c-axis at rather low electric fields below $T^*$. These results suggest some collective excitation contributes to the c-axis charge dynamics of the slightly hole-doped ladders of Sr$_{14}$Cu$_{24}$O$_{41}$ below $T^*$.Comment: 7 pages, 4 figure, to be published in Europhysics Letter

VLBI Observations of Water Masers in the Circumstellar Envelope of IRC+60169

Water masers around an AGB star, IRC+60169, were observed at four epochs using the Japanese VLBI networks. The distribution of the maser features is limited in a thick-shell region, which has inner and outer expansion velocities of 7 km/s and 14 km/s at radii of 25 mas and 120 mas, respectively. The distribution of the red-shifted features exhibits a ring-like structure, the diameter of which is 30 mas, and corresponds to the inner radius of the maser shell. This implies that dense gas around the star obscures red-shifted emission. Although a position--radial velocity diagram for the maser features is consistent with a spherical shell model, the relative proper motions do not indicate an expansion motion of the shell. A remarkable property has been found that is a possible periodic change of the alignment pattern of water maser spots.Comment: 9 pages including 7 figures, to appear in PASJ, Vol. 54, No.

H2O Maser Observations of Candidate Post-AGB Stars and Discovery of Three High-velocity Water Sources

We present the results of 22 GHz H_2O maser observations of a sample of 85 post-Asymptotic Giant Branch (post-AGB) candidate stars, selected on the basis of their OH 1612 MHz maser and far-infrared properties. All sources were observed with the Tidbinbilla 70-m radio telescope and 21 detections were made. 86 GHz SiO Mopra observations of a subset of the sample are also presented. Of the 21 H_2O detections, 15 are from sources that are likely to be massive AGB stars and most of these show typical, regular H_2O maser profiles. In contrast, nearly all the detections of more evolved stars exhibited high-velocity H_2O maser emission. Of the five sources seen, v223 (W43A, IRAS 18450-0148) is a well known `water-fountain' source which belongs to a small group of post-AGB stars with highly collimated, high-velocity H_2O maser emission. A second source in our sample, v270 (IRAS 18596+0315), is also known to have high-velocity emission. We report the discovery of similar emission from a further three sources, d46 (IRAS 15445-5449), d62 (IRAS 15544-5332) and b292 (IRAS 18043-2116). The source d46 is an evolved post-AGB star with highly unusual maser properties. The H_2O maser emission from d62 is probably associated with a massive star. The source b292 is a young post-AGB star that is highly likely to be a water-fountain source, with masers detected over a velocity range of 210 km s^{-1}.Comment: 47 pages, 9 figures, 4 tables, accepted by Ap

The 2006 Radio Outburst of a Microquasar Cyg X-3: Observation and Data

We present the results of the multi-frequency observations of radio outburst of the microquasar Cyg X-3 in February and March 2006 with the Nobeyama 45-m telescope, the Nobeyama Millimeter Array, and the Yamaguchi 32-m telescope. Since the prediction of a flare by RATAN-600, the source has been monitored from Jan 27 (UT) with these radio telescopes. At the eighteenth day after the quench of the activity, successive flares exceeding 1 Jy were observed successfully. The time scale of the variability in the active phase is presumably shorter in higher frequency bands. We also present the result of a follow-up VLBI observation at 8.4 GHz with the Japanese VLBI Network (JVN) 2.6 days after the first rise. The VLBI image exhibits a single core with a size of <8 mas (80 AU). The observed image was almost stable, although the core showed rapid variation in flux density. No jet structure was seen at a sensitivity of $T_b = 7.5\times 10^5$ K.Comment: 17 pages,6 figures; accepted by PAS

Microstructure and kinematics of H2O masers in the massive star forming region IRAS 06061+2151

We have made multi-epoch VLBI observations of H2O maser emission in the massive star forming region IRAS 06061+2151 with the Japanese VLBI network (JVN) from 2005 May to 2007 October. The detected maser features are distributed within an 1\arcsec$\times$1\arcsec (2000 au$\times$2000 au at the source position) around the ultra-compact H {\small\bf II} region seen in radio continuum emission. Their bipolar morphology and expanding motion traced through their relative proper motions indicate that they are excited by an energetic bipolar outflow. Our three-dimensional model fitting has shown that the maser kinematical structure in IRAS 06061+2151 is able to be explained by a biconical outflow with a large opening angle ($>$ 50\degr). The position angle of the flow major axis coincides very well with that of the large scale jet seen in 2.1\:\mu\rmn{m} hydrogen emission. This maser geometry indicates the existence of dual structures composed of a collimated jet and a less collimated massive molecular flow. We have also detected a large velocity gradient in the southern maser group. This can be explained by a very small (on a scale of several tens of au) and clumpy (the density contrast by an order of magnitude or more) structure of the parental cloud. Such a structure may be formed by strong instability of shock front or splitting of high density core.Comment: 14 pages, 6 figures accepted for publication in MNRA

Single-cell RNA-sequencing resolves self-antigen expression during mTEC development

The crucial capability of T cells for discrimination between self and non-self peptides is based on negative selection of developing thymocytes by medullary thymic epithelial cells (mTECs). The mTECs purge autoreactive T cells by expression of cell-type specific genes referred to as tissue-restricted antigens (TRAs). Although the autoimmune regulator (AIRE) protein is known to promote the expression of a subset of TRAs, its mechanism of action is still not fully understood. The expression of TRAs that are not under the control of AIRE also needs further characterization. Furthermore, expression patterns of TRA genes have been suggested to change over the course of mTEC development. Herein we have used single-cell RNA-sequencing to resolve patterns of TRA expression during mTEC development. Our data indicated that mTEC development consists of three distinct stages, correlating with previously described jTEC, mTEChi and mTEClo phenotypes. For each subpopulation, we have identified marker genes useful in future studies. Aire-induced TRAs were switched on during jTEC-mTEC transition and were expressed in genomic clusters, while otherwise the subsets expressed largely overlapping sets of TRAs. Moreover, population-level analysis of TRA expression frequencies suggested that such differences might not be necessary to achieve efficient thymocyte selection.RM is supported by a PhD Fellowship from the Fundação para a Ciência e Tecnologia, Portugal (SFRH/ BD/51950/2012). XZ is supported by an Advanced Postdoc Mobility Fellowship from the Swiss National Science Foundation (SNSF, grant number P300P2_151352). Part of the work was performed during XZ’s visit to the Simons Institute for the Theory of Computing. TL is supported by the Academy of Finland (Decision 311081). The authors would like to thank Bee Ling Ng and the staff of the Cytometry Core Facility, and Stephan Lorenz and the staff of the Single Cell Genomics Core Facility for their contribution. Mark Lynch is acknowledged for technical assistance with the Fluidigm C1 platform. Mike Stubbington and Kylie James are acknowledged for revising the language of the manuscript. We thank Sarah Teichmann for help and discussions regarding the manuscript.info:eu-repo/semantics/publishedVersio

Antimicrobial de-escalation in the critically ill patient and assessment of clinical cure: the DIANA study

Purpose: The DIANA study aimed to evaluate how often antimicrobial de-escalation (ADE) of empirical treatment is performed in the intensive care unit (ICU) and to estimate the effect of ADE on clinical cure on day 7 following treatment initiation. Methods: Adult ICU patients receiving empirical antimicrobial therapy for bacterial infection were studied in a prospective observational study from October 2016 until May 2018. ADE was defined as (1) discontinuation of an antimicrobial in case of empirical combination therapy or (2) replacement of an antimicrobial with the intention to narrow the antimicrobial spectrum, within the first 3&nbsp;days of therapy. Inverse probability (IP) weighting was used to account for time-varying confounding when estimating the effect of ADE on clinical cure. Results: Overall, 1495 patients from 152 ICUs in 28 countries were studied. Combination therapy was prescribed in 50%, and carbapenems were prescribed in 26% of patients. Empirical therapy underwent ADE, no change and change other than ADE within the first 3&nbsp;days in 16%, 63% and 22%, respectively. Unadjusted mortality at day 28 was 15.8% in the ADE cohort and 19.4% in patients with no change [p = 0.27; RR 0.83 (95% CI 0.60\u20131.14)]. The IP-weighted relative risk estimate for clinical cure comparing ADE with no-ADE patients (no change or change other than ADE) was 1.37 (95% CI 1.14\u20131.64). Conclusion: ADE was infrequently applied in critically ill-infected patients. The observational effect estimate on clinical cure suggested no deleterious impact of ADE compared to no-ADE. However, residual confounding is likely