7,050 research outputs found

    A micro cell lysis device

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    A new micromachined cell lysis device is developed. It is designed for miniature bio-analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification. It consists of muti-electrode pairs to apply electric fields to cells. We adopt the means of using electric field lysing because it can greatly simplify purification steps for preparation of biological samples, when compared to conventional chemical methods. Yeast, Chinese cabbage, radish cells and E. coli are tested with the device. The lysis of yeast, Chinese cabbage, radish cells is observed by a microscope. The experimental observation suggests E. coli are also lysed by the pulsed electric field. The range of electric field for the lysis is on the order of 1 kV/cm to 10 kV/cm. In addition, for practical reasons, we reduce the voltage required for lysing to less than 10 V by making the electrode gap on the order of microns

    Flexible parylene actuator for micro adaptive flow control

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    This paper describes the first flexible parylene electrostatic actuator valves intended for micro adaptive flow control for the future use on the wings of micro-air-vehicle (MAV). The actuator diaphragm is made of two layers of parylene membranes with offset vent holes. Without electrostatic actuation, air can move freely from one side of the skin to the other side through the vent holes. With actuation, these vent holes are sealed and the airflow is controlled. The membrane behaves as a complete diaphragm. We have successfully demonstrated this function using a 2-mm x 2-mm parylene diaphragm electrostatic actuator valves. This work also includes the novel anti-stiction technology that is crucial to make such large-area parylene actuator diaphragm with the combined use of anti-stiction posts, self-assembled monolayers (SAM), surface roughening, and bromine trifluoride (BrFe) dry etching. With the help of SAM treatment, the operating voltage is lowered from 30 volts to 13 volts. The load deflection method is then used to measure the effective thickness of the composite diaphragm. The flexible parylene diaphragm can be deflected up to 100 ÎĽm when 150 Torr of pressure is applied. The result is fitted into a theoretical model and yields an effective thickness of 5.9 ÎĽm, which is agreeable with the actual thickness of 5.6 ÎĽm, thus proves the functionality of the device

    Verification of a localization criterion for several disordered media

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    We analytically compute a localization criterion in double scattering approximation for a set of dielectric spheres or perfectly conducting disks uniformly distributed in a spatial volume which can be either spherical or layered. For every disordered medium, we numerically investigate a localization criterion, and examine the influence of the system parameters on the wavelength localization domains.Comment: 30 pages, LateX, amstex, revtex styles, 20 figure
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