A semiquantitative analysis of reactive astrogliosis demonstrates its correlation with the number of intact motor neurons after transient spinal cord ischemia

ObjectiveWe evaluated the relationship between reactive astrogliosis and delayed motor neuron death after transient spinal cord ischemia in rabbits using a semiquantitative analysis of glial fibrillary acidic protein expression.MethodsSpinal cord ischemia was induced by means of balloon occlusion of the infrarenal aorta for 15 minutes at 39°C in 18 New Zealand white rabbits. At 1, 3, and 7 days after reperfusion, 6 animals at each time point were killed, and the spinal cord was removed for histologic and immunohistochemical study. The variables analyzed were (1) neurologic function (Johnson score) at every 24 hours after reperfusion, (2) the number of intact motor neurons and terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate-biotin nick-end labeling–positive positive neurons, and (3) expression of glial fibrillary acidic protein in the gray and white matter, which was expressed as the percentage of stained area.ResultsAll animals presented delayed motor neuron death. The number of intact neurons decreased correlatively with neurologic function. No obvious terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate-biotin nick-end labeling–positive cells were observed. Glial fibrillary acidic protein expression increased with time in both the gray and white matter, representing the development of reactive astrogliosis. Significant correlation was found between glial fibrillary acidic protein expression and the number of intact motor neurons on the third day in both the gray (r2 = 0.726, P = .031) and white (r2 = 0.927, P = .002) matter.ConclusionsReactive astrogliosis 3 days after transient spinal cord ischemia correlates with the number of intact motor neurons. Our method for semiquantitative analysis of reactive astrogliosis is simple and reproducible and seems useful for such experimental studies

Chick-computer interaction using sounds

The 11th International Symposium on Adaptive Motion of Animals and Machines. Kobe University, Japan. 2023-06-06/09. Adaptive Motion of Animals and Machines Organizing Committee.Poster Session P4

Closed conformal Killing-Yano tensor and Kerr-NUT-de Sitter spacetime uniqueness

We study spacetimes with a closed conformal Killing-Yano tensor. It is shown that the D-dimensional Kerr-NUT-de Sitter spacetime constructed by Chen-Lu-Pope is the only spacetime admitting a rank-2 closed conformal Killing-Yano tensor with a certain symmetry.Comment: 7 pages, LaTe

Generalized Kerr-NUT-de Sitter metrics in all dimensions

We classify all spacetimes with a closed rank-2 conformal Killing-Yano tensor. They give a generalization of Kerr-NUT-de Sitter spacetimes. The Einstein condition is explicitly solved and written as an indefinite integral. It is characterized by a polynomial in the integrand. We briefly discuss the smoothness conditions of the Einstein metrics over compact Riemannian manifolds.Comment: 12 pages, LaTeX; v2: Einstein conditions for general type included, with slight modification of text, typos corrected; v3: references added, typos corrected, the version published in Physics Letters

Closed conformal Killing-Yano tensor and geodesic integrability

Assuming the existence of a single rank-2 closed conformal Killing-Yano tensor with a certain symmetry we show that there exist mutually commuting rank-2 Killing tensors and Killing vectors. We also discuss the condition of separation of variables for the geodesic Hamilton-Jacobi equations.Comment: 17 pages, no figure, LaTe

Translations and notes of Dunhuang Quzici I

Yun yao ji 雲謠集 is a collection of Dunhuang quzici 敦煌曲子詞, containing 30 poems. Dunhuang quzici 敦煌曲子詞 is undoubtedly a preceding form of Songci 宋詞, a research on it has not being actively pursued in the Japanese academic world. HASEBE, Tsuyoshi and TACHIBANA, Chihaya translated and annotated 4 poems in Yun yao ji 雲謠集 in cooperation. This is the first stage of study on Dunhuang quzici 敦煌曲子詞.研究ノー

Association of depressive symptomatology and dry-mouth feeling in relation to state of taking medicine in citizens from their 40s to 70s selected randomly from two cities in Hokkaido, Japan

Background: Troponin I (TnI) are highly specific to the myocardium, and measurement of the plasma TnI levels has been widely used to evaluate myocardial damage. This study was performed to ascertain the pathologic or perioperative variables that are significantly associated with the plasma cardiac TnI level. We also intended to provide reference range of TnI elevation after uncomplicated pediatric open heart surgery.Methods: One hundred fifty-one patients undergoing repair of atrial septal defects (ASDs) (n = 61), ventricular septal defects (VSDs) (n = 71), tetralogy of Fallot (TOF) (n = 14), or complete atrioventricular septal defects (CAVSDs) (n = 5) were included. The plasma TnI level was measured prior to surgery, on days 1 and 7 postoperatively. We analyzed the relationship between the TnI level and pathology, cardiopulmonary bypass, and other perioperative actors.Results: All patients underwent uncomplicated surgery and postoperative course. With all pathologies, the plasma TnI level peaked on postoperative day 1 and rapidly declined. The TnI level on postoperative day 1 was higher in VSD than ASD, and there was a further rise in TOF and CAVSD but no significant difference between them. The TnI level on postoperative day 1 was strongly correlated with the operative time, cardiopulmonary bypass time, and aortic cross-clamp (ACC) time. Multiple linear regression analysis showed that the operative time, ACC time, lowest rectal temperature, TOF lesion, and inotropic support period could influence the TnI level.Conclusions: Elevation of plasma TnI after pediatric open heart surgery for various congenital heart diseases is mainly associated with the condition of cardiopulmonary bypass (operative and ACC times) and is not affected with surgical procedures expect for ventricular muscle resection during repair of TOF. Level of TnI elevation confirmed in the study could provide reference range in uncomplicated open heart surgery for each four pathologies. (298 words

Association of depressive symptomatology and dry-mouth feeling in relation to state of taking medicine in citizens from their 40s to 70s selected randomly from two cities in Hokkaido, Japan

Background: Troponin I (TnI) are highly specific to the myocardium, and measurement of the plasma TnI levels has been widely used to evaluate myocardial damage. This study was performed to ascertain the pathologic or perioperative variables that are significantly associated with the plasma cardiac TnI level. We also intended to provide reference range of TnI elevation after uncomplicated pediatric open heart surgery.Methods: One hundred fifty-one patients undergoing repair of atrial septal defects (ASDs) (n = 61), ventricular septal defects (VSDs) (n = 71), tetralogy of Fallot (TOF) (n = 14), or complete atrioventricular septal defects (CAVSDs) (n = 5) were included. The plasma TnI level was measured prior to surgery, on days 1 and 7 postoperatively. We analyzed the relationship between the TnI level and pathology, cardiopulmonary bypass, and other perioperative actors.Results: All patients underwent uncomplicated surgery and postoperative course. With all pathologies, the plasma TnI level peaked on postoperative day 1 and rapidly declined. The TnI level on postoperative day 1 was higher in VSD than ASD, and there was a further rise in TOF and CAVSD but no significant difference between them. The TnI level on postoperative day 1 was strongly correlated with the operative time, cardiopulmonary bypass time, and aortic cross-clamp (ACC) time. Multiple linear regression analysis showed that the operative time, ACC time, lowest rectal temperature, TOF lesion, and inotropic support period could influence the TnI level.Conclusions: Elevation of plasma TnI after pediatric open heart surgery for various congenital heart diseases is mainly associated with the condition of cardiopulmonary bypass (operative and ACC times) and is not affected with surgical procedures expect for ventricular muscle resection during repair of TOF. Level of TnI elevation confirmed in the study could provide reference range in uncomplicated open heart surgery for each four pathologies. (298 words