1,012 research outputs found
Vectors for N- or C-terminal positioning of the yeast Gal4p DNA binding or activator domains
EMTREE drug terms: fungal protein; heat shock protein 90; hybrid protein; transcription factor
EMTREE medical terms: amino terminal sequence; article; carboxy terminal sequence; DNA binding; DNA binding domain; expression vector; Gal4p domain; gene activation; gene activation domain; gene expression; nonhuman; plasmid; plasmid ADC; plasmid BDC; protein domain; protein protein interaction; technique; two hybrid system; yeast
MeSH: Binding Sites; DNA-Binding Proteins; Genetic Vectors; Protein Structure, Tertiary; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Trans-Activation (Genetics); Transcription Factors; Two-Hybrid System Techniqu
The Capaciousness of No: Affective Refusals as Literacy Practices
© 2020 The Authors. Reading Research Quarterly published by Wiley Periodicals, Inc. on behalf of International Literacy Association The authors considered the capacious feeling that emerges from saying no to literacy practices, and the affective potential of saying no as a literacy practice. The authors highlight the affective possibilities of saying no to normative understandings of literacy, thinking with a series of vignettes in which children, young people, and teachers refused literacy practices in different ways. The authors use the term capacious to signal possibilities that are as yet unthought: a sense of broadening and opening out through enacting no. The authors examined how attention to affect ruptures humanist logics that inform normative approaches to literacy. Through attention to nonconscious, noncognitive, and transindividual bodily forces and capacities, affect deprivileges the human as the sole agent in an interaction, thus disrupting measurements of who counts as a literate subject and what counts as a literacy event. No is an affective moment. It can signal a pushback, an absence, or a silence. As a theoretical and methodological way of thinking/feeling with literacy, affect proposes problems rather than solutions, countering solution-focused research in which the resistance is to be overcome, co-opted, or solved. Affect operates as a crack or a chink, a tiny ripple, a barely perceivable gesture, that can persist and, in doing so, hold open the possibility for alternative futures
A Complete Developmental Sequence of a Drosophila Neuronal Lineage as Revealed by Twin-Spot MARCM
Labeling every neuron in a lineage in the fruit fly olfactory system reveals that every cell is born with a pre-determined cell fate that is invariant and dependent upon neuron birth orde
Necessary and sufficient conditions for path-independence of Girsanov transformation for infinite-dimensional stochastic evolution equations
Based on a recent result on linking stochastic differential equations on â d to (finite-dimensional) Burger-KPZ type nonlinear parabolic partial differential equations, we utilize Galerkin type finite-dimensional approximations to characterize the path-independence of the density process of Girsanov transformation for the infinite-dimensional stochastic evolution equations. Our result provides a link of infinite-dimensional semi-linear stochastic differential equations to infinite-dimensional Burgers-KPZ type nonlinear parabolic partial differential equations. As an application, this characterization result is applied to stochastic heat equation in one space dimension over the unit interval
Stochastic Reaction-diffusion Equations Driven by Jump Processes
We establish the existence of weak martingale solutions to a class of second
order parabolic stochastic partial differential equations. The equations are
driven by multiplicative jump type noise, with a non-Lipschitz multiplicative
functional. The drift in the equations contains a dissipative nonlinearity of
polynomial growth.Comment: See journal reference for teh final published versio
Synaptic transmission parallels neuromodulation in a central food-intake circuit
NeuromedinU is a potent regulator of food intake and activity in mammals. In Drosophila, neurons producing the homologous neuropeptide hugin regulate feeding and locomotion in a similar manner. Here, we use EM-based reconstruction to generate the entire connectome of hugin-producing neurons in the Drosophila larval CNS. We demonstrate that hugin neurons use synaptic transmission in addition to peptidergic neuromodulation and identify acetylcholine as a key transmitter. Hugin neuropeptide and acetylcholine are both necessary for the regulatory effect on feeding. We further show that subtypes of hugin neurons connect chemosensory to endocrine system by combinations of synaptic and peptide-receptor connections. Targets include endocrine neurons producing DH44, a CRH-like peptide, and insulin-like peptides. Homologs of these peptides are likewise downstream of neuromedinU, revealing striking parallels in flies and mammals. We propose that hugin neurons are part of an ancient physiological control system that has been conserved at functional and molecular level.SFB 645 and 704, DFG Cluster of Excellence ImmunoSensation, DFG grant PA 787, HHMI Janeli
Burgers' Flows as Markovian Diffusion Processes
We analyze the unforced and deterministically forced Burgers equation in the
framework of the (diffusive) interpolating dynamics that solves the so-called
Schr\"{o}dinger boundary data problem for the random matter transport. This
entails an exploration of the consistency conditions that allow to interpret
dispersion of passive contaminants in the Burgers flow as a Markovian diffusion
process. In general, the usage of a continuity equation , where stands for the
Burgers field and is the density of transported matter, is at variance
with the explicit diffusion scenario. Under these circumstances, we give a
complete characterisation of the diffusive transport that is governed by
Burgers velocity fields. The result extends both to the approximate description
of the transport driven by an incompressible fluid and to motions in an
infinitely compressible medium. Also, in conjunction with the Born statistical
postulate in quantum theory, it pertains to the probabilistic (diffusive)
counterpart of the Schr\"{o}dinger picture quantum dynamics.Comment: Latex fil
Social Preferences and the Efficiency of Bilateral Exchange
Under what conditions do social preferences, such as altruism or a concern for fair outcomes, generate efficient trade? I analyze theoretically a simple bilateral exchange game: Each player sequentially takes an action that reduces his own material payoff but increases the other playerâs. Each playerâs preferences may depend on both his/her own material payoff and the other playerâs. I identify necessary conditions and sufficient conditions on the playersâ preferences for the outcome of their interaction to be Pareto efficient. The results have implications for interpreting the rotten kid theorem, gift exchange in the laboratory, and gift exchange in the field
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