Medical Malpractice Litigation Under National Health Insurance: Essential or Expendable?

Identification of time-continuous models from sampled data is a long standing topic of discussion, and many approaches have been suggested. The Maximum Likelihood method is asymptotically and theoretically superior to other methods. However, it may suffer from numerical inaccuracies at fast sampling and it also requires reliable initial parameter values. A number of efficient and useful alternatives to the maximum-likelihood method have been developed over the years. The most important of these are State-Variable filters, combined with Instrumental Variable methods, including the simplified refined IV method. In this contribution we perform unpretentious numerical experiments to comment on these methods, and their mutual benefits.CADIC

Ecological Stoichiometry: A Link Between Developmental Speed and Physiological Stress in an Omnivorous Insect

The elemental composition of organisms belongs to a suite of functional traits that may adaptively respond to fluctuating selection pressures. Life history theory predicts that predation risk and resource limitations impose selection pressures on organisms' developmental time and are further associated with variability in energetic and behavioral traits. Individual differences in developmental speed, behaviors and physiology have been explained using the pace-of-life syndrome (POLS) hypothesis. However, how an organism's developmental speed is linked with elemental body composition, metabolism and behavior is not well understood. We compared elemental body composition, latency to resume activity and resting metabolic rate (RMR) of western stutter-trilling crickets (Gryllus integer) in three selection lines that differ in developmental speed. We found that slowly developing crickets had significantly higher body carbon, lower body nitrogen and higher carbon-to-nitrogen ratio than rapidly developing crickets. Slowly developing crickets had significantly higher RMR than rapidly developing crickets. Male crickets had higher RMR than females. Slowly developing crickets resumed activity faster in an unfamiliar relative to a familiar environment. The rapidly developing crickets did the opposite. The results highlight the tight association between life history, physiology and behavior. This study indicates that traditional methods used in POLS research should be complemented by those used in ecological stoichiometry, resulting in a synthetic approach that potentially advances the whole field of behavioral and physiological ecology

Microbiome symbionts and diet diversity incur costs on the immune system of insect larvae.

Communities of symbiotic microorganisms that colonize the gastrointestinal tract play an important role in food digestion and protection against opportunistic microbes. Diet diversity increases the number of symbionts in the intestines, a benefit that is considered to impose no cost for the host organism. However, less is known about the possible immunological investments that hosts have to make in order to control the infections caused by symbiont populations that increase because of diet diversity. Using taxonomical composition analysis of the 16S rRNAV3 region, we show that enterococci are the dominating group of bacteria in the midgut of the larvae of the greater wax moth (Galleria mellonella). We found that the number of colony-forming units of enterococci and expressions of certain immunity-related antimicrobial peptide (AMP) genes such as Gallerimycin, Gloverin, 6-tox, Cecropin-D and Galiomicin increased in response to a more diverse diet, which in turn decreased the encapsulation response of the larvae. Treatment with antibiotics significantly lowered the expression of all AMP genes. Diet and antibiotic treatment interaction did not affect the expression of Gloverin and Galiomicin AMP genes, but significantly influenced the expression of Gallerimycin, 6-tox and Cecropin-D. Taken together, our results suggest that diet diversity influences microbiome diversity and AMP gene expression, ultimately affecting an organism's capacity to mount an immune response. Elevated basal levels of immunity-related genes (Gloverin and Galiomicin) might act as a prophylactic against opportunistic infections and as a mechanism that controls the gut symbionts. This would indicate that a diverse diet imposes higher immunity costs on organisms

Skin and skeletal system lesions of european pond turtles (Emys orbicularis) from natural habitats

Water pollution is known to play an important role in the pathogenesis of plastron, carapace and skin diseases of turtles. In this study, a total of 150 European pond turtles (Emys orbicularis) of different age and both sexes, originating from natural habitats in Serbia, were examined for morphological changes of the skin, plastron, carapace and skeletal system. The turtles were taken out from their natural habitats in Lake Ludas, Lake Palic and Lake Tresetiste. After artificial hibernation, they were subjected to detailed examination, sampled and treated, and finally returned into their natural habitat. Biopsies from the skin and shell were subjected to histopathological examination and microbiological analysis. X-ray scanning was also performed to detect changes in the skeletal system. Macroscopic changes of the skin, most frequently degenerative, inflammatory or neoplastic diseases, were diagnosed in 49.33% of the turtles examined. Dermatitis of different origin and form was the most prominent histopathological finding (28.00%). In the plastron, inflammatory and degenerative processes were frequently found. Osteopathy and mechanical injuries were the dominant findings. Macroscopic changes of the plastron, carapace and skeletal system were diagnosed in 67.33% of the turtles examined. Using X-ray scanning, generalised osteopathy, anomalies and malformations of different aetiology were also diagnosed on the tail and legs. Microbiological examinations showed the presence of a variety of bacterial and fungal agents, either primary pathogens or potential polluters, which invaded the skin and shell, or were present in cloacal swab samples. Bacterial infection was diagnosed in 76.66% of the turtles, first of all in those with skin and shell necrosis. Mycoses were diagnosed in 33.33% of the animals

Do biological traits drive geographic patterns in European amphibians?

Aims: The contemporary biogeography of European amphibians has been hypothesized to have arisen from range expansion and recolonization of the northern part of the continent from southern late Pleistocene refugia, such that northern species generally possess large ranges, while southerly species are mostly small-ranged. Here, we test the hypothesis that these patterns are likely to be underpinned by biological traits associated with dispersal ability, by means of analyses of data for anurans and urodeles, the two main groups of European amphibians. Location: Europe. Methods: We built a database of biological traits (body size, fecundity, life span, habitat specialization) of European amphibians, excluding island endemics. We mapped basic macroecological patterns of range size and position, and analysed the causal pathways for range size using structural equation models (SEM). Results: Amphibian species of small-range size are largely restricted to areas in Southern Europe associated with putative Pleistocene refugia. Those present in Northern Europe are exclusively large-ranged species whose distributions extend all the way from Southern Europe. SEMs explained 54% of range size variation for anurans, with long life span and high fecundity being influential explanatory variables, and explained 61% of range size variation within urodeles, with measures of species fecundity influential. Main conclusions: Species that have successfully recolonized the north following deglaciation have the largest ranges, for both groups of amphibians. These large-ranged species generally possess traits that indicate rapid range expansion potential, with differences apparent in the balance of traits between anurans and urodeles. The traits linked to northern distributions (and large range size) appear to be a mix of r and K traits, indicating that intermediate life history strategies have proved to be optimal for range expansion into northern regions. These results integrate species biology with geographical history in explaining contemporary patterns of species distribution, range size and diversity

Brain stem cell division and maintenance studied using multi-isotope imaging mass spectrometry (MIMS)

New neurons are continuously produced from neural stem cells in specific regions of the adult brain of animals and humans. In the hippocampus, a region crucial for cognitive function, neurogenesis responds to a multitude of extrinsic stimuli; emerging evidence indicates that it may be important for behavior, pathophysiology, brain repair, and response to drugs. We have developed an approach to identify and quantify the cellular targets of pro- and anti-neurogenic stimuli, based on reporter transgenicmouse lines in which neural stemand progenitor cells or their progeny aremarked by fluorescent proteins. Here, we demonstrate the feasibility of using multi-isotope imaging mass spectrometry for studying adult neurogenesis