Cloning, high-level expression, purification and characterization of a staphylokinase variant, SakøC, from Staphylococcus aureus QT08 in Escherichia coli BL21

The staphylokinase (Sak) is emerging as an important thrombolytic agent for the treatment of patients suffering from cardiovascular disease. Hence in this study, we reported the cloning, high-level expression, purification and characterization of the Sak variant SakøC from Staphylococcus aureus QT08 in Escherichia coli Bl21. The sak gene of 489 bp encoding a protein (163 amino acids) with a predicted molecular mass of 18.5 kDa and pI 7.28 showed 99.8 to 99.6% identity with corresponding sequences from S. aureus strains deposited in GenBank (AF332619, X00127, EF122253 and M57455). The DNA sequence (411 bp) encoding the mature Sak (15.5 kDa) truncated 27 N-terminal amino acids was expressed in E. coli BL21/pESak under the control of the strong promoter tac in the presence of isopropyl-β-D-1-thiogalactopynoside (IPTG) as inducer. The expression level of rSak was estimated at about 42% of the total cellular proteins by densitometry scanning, which is the highest expression level of rSak expressed in any E. coli system. The recombinant staphylokinase was purified by Ni2+- ProBondTM column to a single homogeneous 16-kDa band on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) with a specific activity of 15175 U/mg protein, a recovery yield of 58% and a purification factor of 2.56. The optimal pH and temperature for the rSak activity was 9 and 37°C, respectively. rSak was stable over a temperature range of 25 to 50°C and at pH range of 7 to 9. Metal ions and detergents also showed an inhibitory effect on rSak, especially Zn2+ and Cu2+ which completely inhibited the enzymatic activity.Key words: Staphylococcus aureus QT08, staphylokinase, cloning, high-level expression, purification, characterization

Special Issue: Real World Application of SHM in Australia

Australian Network of Structural Health Monitoring (ANSHM) was established in 2009 to promote and advance the field of SHM in Australia and the association has grown considerably since then. By November of 2018, ANSHM has the membership made of 45 organisations including 20 universities, 16 private companies, 6 road authorities and 3 research institutions. Every year ANSHM organises an annual workshop and/or conference sessions for members to exchange their research and practical developments in SHM. One edited book and nine journal special issues have been produced since the establishment of ANSHM. One of these special issues was organised in Structural Health Monitoring - an International Journal (SHMIJ) in 2014. On 6–7 December 2017, ANSHM held its 9th annual workshop as part of the prestigious 8th International Conference on Structural Health Monitoring of Intelligent Infrastructures (SHMII-8) in Brisbane, Queensland, Australia. The main focus of both SHMII-8 and the 9th ANSHM workshop was SHM in real-world application. Interestingly, all sessions of SHMII-8 and ANSHM workshop were held within the P block building at Gardens Point Campus of Queensland University of Technology (QUT) that was instrumented with Australia's first ever long-term full-scale SHM system. Inspired by this theme and high-quality presentations at the workshop, a special issue named 'Real World Application of SHM in Australia' was established in SHMIJ and the 9th ANSHM workshop speakers were invited to submit enhanced and extended versions of their papers to this Special Issue. After rigorous pre-screening, peer review and revision processes, fourteen papers were accepted for inclusion in the Special Issue. The contributions include deterioration assessment of the instrumented P block building at QUT using hybrid model updating and long-term vibration monitoring data, reliability-based load-carrying capacity assessment of bridges using SHM and non-linear analysis, and innovative vibration based damage identification methods with applications to cable-stayed, steel-truss or timber bridges as well as to frame, utility-pole or building structures. The Special Issue also includes new research on non-destructive evaluation of (i) incipient pitting corrosion in reinforced concrete structures, (ii) gaps between carbon fibre reinforced polymer composite and concrete surfaces, (iii) fatigue cracks in pipes, (iv) bolted joints, and (v) in-situ stress. Most studies were verified on real civil structures or large-scale laboratory models well reflecting the high applicability of the developed methods to solve real-world problems. As the guest editors of this Special Issue, we thank the authors for their contribution and all the anonymous reviewers who provided constructive review comments to the manuscripts submitted to this Special Issue. We would also like to express our sincere gratitude to the Managing Editor Professor Michael Todd and the journal executive committee for their support and assistance during the submission and review process. Finally, we would like to thank the SAGE Publications team for their diligence in assuring the efficient and timely production of the papers toward the publication of this Special Issue

System dynamics modelling and its implications for childhood obesity prevention: evidence from improving the consumption of portion size and meal frequency

The childhood obesity has rapidly increased, though little is known about the impact of frequency of meals and portion size on weight and obesity. The objective of this study is to compare and to determine the effective strategy for obesity prevention by improving the consumption of portion size and meal frequency. This study utilised the secondary data obtained from the Health Survey for England for the child population aged between 2 to 15 years in United Kingdom. This study combines the different strands of knowledge from nutrition, physical activity and body metabolism and synthesizing this knowledge into a system dynamics model which the model offers unique insights into the cause-and-effect relationships among the influencing factors. Findings from the simulation analysis demonstrated that reducing meal frequency is the most effective controlling strategy for obesity prevention. This is asserted by the fact that the highest reduction in average weight (3.14%-4.5%) and average body mass index (3.14%-4.5%) between 2020 and 2030 was observed by improving meal frequency. This paper concludes that system dynamics utilised in this study will be advantageous to guide the food stakeholder to gain insight into the complex of eating behavior and to experiment with various intervention strategies for obesity prevention

The relationship between resistant starch and glycemic control: A review on current evidence and possible mechanisms

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Model informed quantification of the feed-forward stimulation of growth hormone by growth hormone-releasing hormone

Aims: Growth hormone (GH) secretion is pulsatile and secretion varies highly between individuals. To understand and ultimately predict GH secretion, it is important to first delineate and quantify the interaction and variability in the biological processes underlying stimulated GH secretion. This study reports on the development of a population nonlinear mixed effects model for GH stimulation, incorporating individual GH kinetics and the stimulation of GH by GH-releasing hormone (GHRH). Methods: Literature data on the systemic circulation, the median eminence, and the anterior pituitary were included as system parameters in the model. Population parameters were estimated on data from 8 healthy normal weight and 16 obese women who received a 33 μg recombinant human GH dose. The next day, a bolus injection of 100 μg GHRH was given to stimulate GH secretion. Results: The GH kinetics were best described with the addition of 2 distribution compartments with a bodyweight dependent clearance (increasing linearly from 24.7 L/h for a 60-kg subject to 32.1 L/h for a 100-kg subject). The model described the data adequately with high parameter precision and significant interindividual variability on the GH clearance and distribution volume. Additionally, high variability in the amount of secreted GH, driven by GHRH receptor activation, was identified (coefficient of variation = 90%). Conclusion: The stimulation of GH by GHRH was quantified and significant interindividual variability was identified on multiple parameters. This model sets the stage for further development of by inclusion of additional physiological components to quantify GH secretion in humans

Perbedaan Kualitas Pelayanan Kesehatan Antara Peserta BPJS Kesehatan PBI Dan Non PBI Kelas 3 Rawat Inap Di RSUD Dr Suratno Gemolong Sragen

The progress of a nation is sustained by strong human resources. To have strong human resources there are three main pillars: health, education, and economy. Health one of the pillars many obstacles to achieve optimal and optimal health degree. Insurance is a health financing effort to overcome the obstacles. BPJS health is a strategic effort to achieve financing in the health sector, especially for poor and disadvantaged groups. This group of health BPJS mostly finance in third grade both PBI and non PBI. With services that responsisiveness, reliability, ansurance, empathy and tangibility then the quality of health services can achieve optimal and maximum health status. In this research use quantitative research method of random sampling and crosssectional observation by using questionnaire with likert scale with validity and reliability test, normality classic assumption test, outlier and homogeneity. For data analysis using compare mean t test Result of data analysis with Ho; difference of health service quality between BPJS healthcare PBI and non PBI 3rd class inpatient RSUD dr Suratno Gemolong Sragen. Results of data with SPSS program obtained 0.239. The significant number is more than 0.05, so Ho> 0,05 so the hypothesis is accepted. The conclusion is that there is a difference of health service quality between BPJS participants of PBI and non PBI grade 3 inpatient of RSUD Gemolong, Srage

Fiber Bragg grating strain modulation based on nonlinear string transverse-force amplifier

2012-2013 > Academic research: refereed > Publication in refereed journalpublished_fina

Very sensitive fiber Bragg grating accelerometer using transverse forces with an easy over-range protection and low cross axial sensitivity

2013-2014 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Analisis Penerapan Lean Hospital Terhadap Kepuasan Konsumen Rumah Sakit PKU Muhammadiyah Surakarta

Hospital health service business is currently considered inefficient because there are still many wastes found in its business processes. Lean hospital is a series of concepts, principles and tools used to eliminate waste and create activities that add value (value added) seen from the perspective of consumers. The purpose of this study was to analyze the effect of lean hospital variables consisting of quality, cost, delivery, safety. morals and the environment towards consumer satisfaction PKU Muhammadiyah Hospital Surakarta. This type of research is quantitative research. The study sample was 130 inpatients. Data collection using questionnaires and multiple linear regression analysis. The results showed that the variables of service quality, cost, safety, morals and environment had a positive and significant effect on patient satisfaction, while the delivery variable had a positive but not significant effect on patient satisfactionKeywords: Lean Hospital, Waste, Value Added, Consumer Satisfaction Keywords: Lean Hospital, Waste, Value Added, Consumer Satisfactio