Evaluation of the Seismic Performance of Light Gauge Steel Walls Braced with Flat Straps

The development of light weight steel structures in seismic area as Italy requires the upgrading of National Codes. To this end, in the last years a theoretical and experimental study was carried out at the University of Naples within the research project RELUIS-DPC 2010-2013. The study focused on all steel design solutions and investigated the seismic behaviour of strap braced stud shear walls. Three wall configurations were defined according to both elastic and dissipative design criteria for three different seismic scenarios. The lateral in-plane behavior of these systems were evaluated by 12 tests performed on full-scale CFS strap-braced stud wall specimens with dimensions 2.4 m x 2.7 m subjected to monotonic and reversed cyclic loading protocols. The experimental campaign was completed with 17 tests on materials, 8 shear tests on elementary steel connections and 28 shear tests on strap-framing connection systems. On the basis of the experimental results, and taking into account the AISI S213 provisions, behaviour factors were evaluated. This paper provides the main outcomes of the experimental tests on walls and behaviour factors evaluation

Experimental Tests for the Seismic Response Evaluation of Cold-Formed Steel Shear Walls Sheathed with Nailed Gypsum Boards

The European project named Energy Efficient LIghtweight-Sustainable-SAfe- Steel Construction (Project acronym: ELISSA) is devoted to the development and demonstration of cold-formed steel (CFS) modular systems. In particular, these systems are nano-enhanced prefabricated lightweight steel skeleton/dry wall construction with improved thermal, vibration/seismic and fire performance, resulting from the inherent thermal, damping and fire spread prevention properties. The different building performances are studied and improved by means of experimental and numerical activities organized on three scale levels: micro-scale, meso-scale and macro-scale. In particular, the evaluation of the seismic performance is carried out at the University of Naples by means tests on connections (micro), seismic-resistant systems (meso) and full-scale two stories house prototype (macro). From a structural point of view, the system is a sheathed-braced CFS solution, in which the seismic resistant elements are made of CFS stud shear walls laterally braced by gypsum-based panels. In the adopted solution, the sheathing panels are attached to the CFS frame by means of ballistic nails, whereas clinching points are used for steel-to-steel connections. The present paper illustrates the results of meso-scale tests performed on four full scale shear walls, in which the influence of the aspect ratio, the type of loading and the effect of finishing was investigated

HMGA1 overexpression is associated with a particular subset of human breast carcinomas

Breast cancer represents the second leading cause of cancer mortality among American women and accounts for more than 40 000 deaths annually. High-mobility group A1 (HMGA1) expression has been implicated in the pathogenesis and progression of human malignant tumours, including breast carcinomas. The aim of this study was to evaluate HMGA1 detection as an indicator for the diagnosis and prognosis of human breast carcinoma

Metabolic reprogramming identifies the most aggressive lesions at early phases of hepatic carcinogenesis

Metabolic changes are associated with cancer, but whether they are just bystander effects of deregulated oncogenic signaling pathways or characterize early phases of tumorigenesis remains unclear. Here we show in a rat model of hepatocarcinogenesis that early preneoplastic foci and nodules that progress towards hepatocellular carcinoma (HCC) are characterized both by inhibition of oxidative phosphorylation (OXPHOS) and by enhanced glucose utilization to fuel the pentose phosphate pathway (PPP). These changes respectively require increased expression of the mitochondrial chaperone TRAP1 and of the transcription factor NRF2 that induces the expression of the rate-limiting PPP enzyme glucose-6-phosphate dehydrogenase (G6PD), following miR-1 inhibition. Such metabolic rewiring exclusively identifies a subset of aggressive cytokeratin-19 positive preneoplastic hepatocytes and not slowly growing lesions. No such metabolic changes were observed during non-neoplastic liver regeneration occurring after two/third partial hepatectomy. TRAP1 silencing inhibited the colony forming ability of HCC cells while NRF2 silencing decreased G6PD expression and concomitantly increased miR-1; conversely, transfection with miR-1 mimic abolished G6PD expression. Finally, in human HCC patients increased G6PD expression levels correlates with grading, metastasis and poor prognosis. Our results demonstrate that the metabolic deregulation orchestrated by TRAP1 and NRF2 is an early event restricted to the more aggressive preneoplastic lesions

Seismic response of Cfs strap-braced stud walls: Experimental investigation

The development of light weight steel structures in seismic area as Italy requires the upgrading of National Codes. To this end, in the last years a theoretical and experimental study was undertaken at the University of Naples within the Italian research project RELUIS-DPC 2010–2013. The study focused on “all-steel design” solutions and investigated the seismic behavior of strap-braced stud walls. Three typical wall configurations were defined according to both elastic and dissipative design criteria for three different seismic scenarios. The lateral in-plane inelastic behavior of these systems was evaluated by twelve tests performed on full-scale Cold-formed strap-braced stud wall specimens with dimensions 2400×2700 m2 subjected to monotonic and reversed cyclic loading protocols. The experimental campaign was completed with seventeen tests on materials, eight shear tests on elementary steel connections and twenty-eight shear tests on strap-framing connection systems. This paper provides the main outcomes of the experimental investigation. Furthermore, the design prescriptions, with particular reference to the behavior factor and the capacity design rules for these systems, have been proved on the basis of experimental results

The role of HMGA1 protein in gastroenteropancreatic neuroendocrine tumors

Neuroendocrine tumors (NETs) are neoplasms derived from neuroendocrine cells. One of their main features is to often remain asymptomatic and clinically undetectable. High Mobility Group A (HMGA) proteins belong to a family of non-histone chromatinic proteins able to modulate gene expression through the interaction with DNA and transcription factors. They are overexpressed in most of the human malignancies, playing a critical role in carcinogenesis. However, their expression levels and their role in neuroendocrine carcinogenesis has not been exhaustively evaluated until now. Therefore, in this study, we have addressed the validity of using the expression of HMGA1 as a diagnostic marker and have investigated its role in NET carcinogenesis. The expression of HMGA1 has been evaluated by qRT-PCR and immunohistochemistry, using NET tissue microarrays, in a cohort of gastroenteropancreatic (GEP)-NET samples. The expression levels of HMGA1 have been then correlated with the main clinical features of NET samples. Finally, the contribution of HMGA1 overexpression to NET development has been addressed as far as the modulation of proliferation and migration abilities of NET cells is concerned. Here, we report that HMGA1 is overexpressed in GEP-NET samples, at both mRNA and protein levels, and that the silencing of HMGA1 protein expression interferes with the ability of NET cells to proliferate and migrate through the downregulation of Cyclin E, Cyclin B1 and EZH2. These results propose the HMGA proteins as new diagnostic and prognostic markers

RNASeq analysis reveals biological processes governing the clinical behaviour of endometrioid and serous endometrial cancers

BACKGROUND: Endometrial carcinoma comprises a group of tumors with distinct histologic and molecular features, and clinical behavior. Here we sought to define the biological processes that govern the clinical behavior of endometrial cancers. METHODS: Sixteen prototype genes representative of different biological processes that would likely play a role in endometrial and other hormone-driven cancers were defined. RNA-sequencing gene expression data from 323 endometrial cancers from The Cancer Genome Atlas were used to determine the transcription module of each prototype gene. The expression of prototype genes and modules and their association with outcome was assessed in univariate and multivariate survival analyses. The association of MSH6 expression with outcome was validated in an independent cohort of 243 primary endometrial cancers using immunohistochemistry. RESULTS: We observed that the clinical behavior of endometrial carcinomas as a group was associated with hormone receptor signaling, PI3K pathway signaling and DNA mismatch repair processes. When analyzed separately, in endometrioid carcinomas, hormone receptor, PI3K and DNA mismatch repair modules were significantly associated with outcome in univariate analysis, whereas the clinical behavior of serous cancers was likely governed by apoptosis and Wnt signaling. Multivariate survival analysis revealed that MSH6 expression was associated with outcome of endometrial cancer patients independently from traditional prognostic clinicopathologic parameters, which was confirmed in an independent cohort at the protein level. CONCLUSION: Endometrioid and serous endometrial cancers are underpinned by distinct molecular pathways. MSH6 expression levels may be associated with outcome in endometrial cancers as a group

Variants of the serotonin transporter gene and NEO-PI-R Neuroticism: No association in the BLSA and SardiNIA samples

The polymorphism in the serotonin transporter gene promoter region (5-HTTLPR) is by far the most studied variant hypothesized to influence Neuroticism-related personality traits. The results of previous studies have been mixed and appear moderated by the personality questionnaire used. Studies that used the TCI to assess Harm Avoidance or the EPQ to assess Neuroticism have found no association with the 5-HTTLPR. However, studies that used the NEO-PI-R or related instruments (NEO-PI, NEO-FFI) to measure Neuroticism have found some evidence of association. This study examines the association of variants in the serotonin transporter gene in a sample from a genetically isolated population within Sardinia (Italy) that is several times larger than previous samples that used the NEO-PI-R (N = 3,913). The association was also tested in a sample (N = 548) from the Baltimore Longitudinal Study of Aging (BLSA), in which repeated NEO-PI-R assessments were obtained. In the SardiNIA sample, we found no significant association of the 5-HTTLPR genotypes with Neuroticism or its facets (Anxiety, Angry-Hostility, Depression, Self-Consciousness, Impulsiveness, and Vulnerability). In the BLSA sample, we found lower scores on Neuroticism traits for the heterozygous group, which is inconsistent with previous studies. We also examined eight SNPs in the SardiNIA (N = 3,972) and nine SNPs in the BLSA (N = 1,182) that map within or near the serotonin transporter gene (SLC6A4), and found no association. Along with other large studies that used different phenotypic measures and found no association, this study substantially increases the evidence against a link between 5-HTT variants and Neuroticism-related traits. © 2009 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/64573/1/30932_ftp.pd

Seismic response of Cfs strap-braced stud walls: Theoretical study

The use of cold-formed steel (CFS) profiles in low-rise residential buildings has increased in European construction sector. The reason of this interest is related to potentialities offered by this constructive system, which are the high structural performance, lightness, short construction time, durability and eco-efficiency. Nevertheless, the current structural codes, such as Eurocodes, do not provide enough information about the seismic design of this structural typology. In an effort to investigate the seismic response of CFS structures, a theoretical and experimental research has been carried out at University of Naples Federico II, with the main aim to support the spreading of these systems in seismic areas. This study focuses on an “all-steel design” solution in which strap-braced stud walls are the main lateral resisting system. In the present paper the outcomes of theoretical phase are shown with the aim of defining the criteria for the seismic design of such structures. In particular, a critical analysis of the requirements for CFS systems provided by the American code AISI S213 has been carried out by comparing it with those given by Eurocodes for traditional braced steel frames