Politics Of Life Itself And The Future Of Medical Practices: Dialogues With Nikolas Rose (part 3)

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Yield and morphology of Nopalea cochenillifera under N fertilization and biological inoculation

Received: August 15th, 2023 ; Accepted: October 30th, 2023 ; Published: November 9th, 2023 ; Correspondence: [email protected]; [email protected] objective was to evaluate the effect of inoculation with Azospirillum brasilense and levels of nitrogen fertilization on the productive aspects of the spineless cactus (Nopalea cochenillifera). The experiment was carried in Ribeirão do Largo, Southwest region of the state of Bahia, from April 2019 to April 2020. The experiment was carried out in a 2×4 factorial, in a randomized block design with eight treatments and four replications. The treatments consisted of the absence or inoculation with Azospirillum brasilense and nitrogen fertilization levels 0, 50, 100 and 150 kg– ¹ of N ha–¹ . There was a positive effect for the use of the bacteria on cladode area index, height, number of secondary, tertiary and total cladodes, total weight for cladodes of all orders and also for final weight per ha–¹ . There was a significant effect of the use of the inoculant for the levels of total soluble sugars and starch, where there was a decrease in the values for the treatments submitted to the use of the bacteria. There was a quadratic effect for the weight of the tertiary cladodes, number of tertiary cladodes and total of spineless cactus when submitted to nitrogen fertilization levels. There was a linear test for cladodes area index when nitrogen fertilization was used. The use of Azospirillum brasilense is positive for the cultivation of spineless cactus, improving development and increasing crop productivity

Apoio Em Rede: A Rede Humaniza Sus Conectando Possibilidades No Ciberespaço

This paper presents the HUmanizaSUS Network (Rede HumanizaSUS) as a virtual environment connecting practices and knowledge about SUS (Brazilian National Health System), conveying the support function in a network. Based on the theoretical framework of Collective Intelligence, we present some dimensions of support experienced on Rede HumanizaSUS as a network intervention technology for strengthening the virtual environment of SUS or CiberespaSUS. © 2016, Fundacao UNI Botucatu/UNESP. All rights reserved.205733734

Screening, production and biochemical characterization of a new fibrinolytic enzyme produced by Streptomyces sp. (Streptomycetaceae) isolated from Amazonian lichens

Thrombosis is a pathophysiological disorder caused by accumulation of fibrin in the blood. Fibrinolytic proteases with potent thrombolytic activity have been produced by diverse microbial sources. Considering the microbial biodiversity of the Amazon region, this study aimed at the screening, production and biochemical characterization of a fibrinolytic enzyme produced by Streptomyces sp. isolated from Amazonian lichens. The strain Streptomyces DPUA1576 showed the highest fibrinolytic activity, which was 283 mm2. Three variables at two levels were used to assess their effects on the fibrinolytic production. The parameters studied were agitation (0.28 - 1.12 g), temperature (28 - 36 ºC) and pH (6.0 - 8.0); all of them had significant effects on the fibrinolytic production. The maximum fibrinolytic activity (304 mm2) was observed at 1.12 g, 28 ºC, and pH of 8.0. The crude extract of the fermentation broth was used to assess the biochemical properties of the enzyme. Protease and fibrinolytic activities were stable during 6 h, at a pH ranging from 6.8 to 8.4 and 5.8 to 9.2, respectively. Optimum temperature for protease activity ranged between 35 and 55 °C, while the highest fibrinolytic activity was observed at 45 ºC. Proteolytic activity was inhibited by Cu2+ and Co2+ ions, phenylmethylsulfonyl fluoride (PMSF) and pepstatin A, which suggests that the enzyme is a serine protease. Enzymatic extract cleaved fibrinogen at the subunits A-chain, A-chain, and -chain. The results indicated that Streptomyces sp. DPUA 1576 produces enzymes with fibrinolytic and fibrinogenolytic activity, enzymes with an important application in the pharmaceutical industry.The authors grateful acknowledge the financial support of Fundação de Amparo a Pesquisa do Estado de Pernambuco (FACEPE, Pernambuco, Brazil, N. 0158-2.12/11), CNPq/ RENORBIO (National Counsel of Technological and Scientific Development, N.55146/2010-3) and National Council for the Improvement of Higher Education (CAPES, Brazil) for the scholarship. The author thanks editor and reviewers for their review and comments.info:eu-repo/semantics/publishedVersio