Assessing temporary speed restrictions and associated unavailability costs in railway infrastructure

This paper analyses the occurrence of temporary speed restrictions in railway infrastructure associated with railway track geometry degradation. A negative binomial regression model is put forward to estimate the expected number of temporary speed restrictions, controlling for the main quality indicators of railway track geometry degradation and for the maintenance and renewal actions/decisions. The prediction of temporary speed restrictions provides a quantitative way to support the assessment of unavailability costs to railway users. A case study on the Lisbon–Oporto Portuguese line is explored, comparing three statistical models: the Poisson, the ‘over-dispersed’ Poisson and the proposed negative binomial regression. Main findings suggest that the main quality indicators for railway track geometry degradation are statistically significant variables, apart from the maintenance and renewal actions. Finally, a discussion on the impacts of the unavailability costs associated with temporary speed restrictions is also provided in a regulated railway context.info:eu-repo/semantics/acceptedVersio

Introdução e avaliação de forrageiras no município de Santana do Araguaia, Estado do Pará.

bitstream/item/32017/1/CPATU-BP115.pd

Methotrexate and Cyclosporine Treatments Modify the Activities of Dipeptidyl Peptidase IV and Prolyl Oligopeptidase in Murine Macrophages

Analysis of the effects of cyclosporine A (25–28 mgkg−1) and/or methotrexate (0.1 mgkg−1) treatments on dipeptidyl peptidase IV (DPPIV) and prolyl oligopeptidase (POP) activities and on algesic response in two distinct status of murine macrophages (Mφs) was undertaken. In resident Mφs, DPPIV and POP were affected by neither individual nor combined treatments. In thioglycolate-elicited Mφs, methotrexate increased DPPIV (99–110%) and POP (60%), while cyclosporine inhibited POP (21%). Combined treatment with both drugs promoted a rise (51–84%) of both enzyme activities. Only cyclosporine decreased (42%) the tolerance to algesic stimulus. Methotrexate was revealed to exert prevalent action over that of cyclosporine on proinflammatory Mφ POP. The opposite effects of methotrexate and cyclosporine on POP activity might influence the availability of the nociceptive mediators bradykinin and substance P in proinflammatory Mφs. The exacerbated response to thermally induced algesia observed in cyclosporine-treated animals could be related to upregulation of those mediators

Injection locking of a low cost high power laser diode at 461 nm

Stable laser sources at 461 nm are important for optical cooling of strontium atoms. In most existing experiments this wavelength is obtained by frequency doubling infrared lasers, since blue laser diodes either have low power or large emission bandwidths. Here, we show that injecting less than 10 mW of monomode laser radiation into a blue multimode 500 mW high power laser diode is capable of slaving at least 50% of the power to the desired frequency. We verify the emission bandwidth reduction by saturation spectroscopy on a strontium gas cell and by direct beating of the slave with the master laser. We also demonstrate that the laser can efficiently be used within the Zeeman slower for optical cooling of a strontium atomic beam.Comment: 2nd corrected version (minor revisions); Manuscript accepted for publication in Review of Scientific Instruments; 5 pages, 6 figure

Modelling of friction stir welding and its influence on the structural behaviour of aluminium stiffened panels

This work deals with the modelling and numerical simulation of aluminium stiffened panels, assembled by means of friction stir welding (FSW) operations, and subjected to compressive loads that can catastrophically induce buckling (global or local) unstable modes and, subsequently, overall failure. Due to their geometrical complexity, added to localized thermo-mechanical effects that typically come from joining by welding, approximation methods such as the Finite Element Method are typically used in such nonlinear analyses, having proved to be useful to designers focused on the prediction of the behaviour (before and post buckling) of such structures. Being grounded on previous contributions from the authors, the present paper aims to bring a number of innovative aspects to the current state of the art by considering in the developed models the simultaneous influence of residual stress fields, material softening effects and geometrical imperfections. Doing so, it is possible to infer about the singular and combined influence of these effects in the overall structural performance of stiffened panels, building up a useful and comprehensive methodology for design stages

Numerical simulation of the buckling behaviour of stiffened panels: Benchmarks for assessment of distinct modelling strategies

This work deals with the effective modelling and simulation of the behavior of stiffened panels, when subjected to compressive (buckling) loads. Within the Finite Element Method, two numerical strategies are compared, namely the Riks method and the displacement incremental control method, including damping effects. The capabilities and limitations of both approaches are explored for two distinct benchmarks: a panel with a blade stiffener, and a panel with a T shaped stiffener. In both cases, material (plasticity) and geometrical (large displacements) nonlinearities are considered, together with a modelling strategy based on shell elements. Following previous works of the authors, each panel accounts for initial geometric imperfections coming from friction stir welding joining operations. The paper shows a number of considerations that must be undertaken when choosing between one of the two modelling strategies. Both benchmarks involve a number of challenges from the point of view of modelling unstable structural behaviors, and therefore the proposed benchmarks can represent a valid set of case studies in the understanding of the capabilities of current numerical simulation codes

Prediction of Friction Stir Welding effects on AA2024-T3 plates and stiffened panels using a shell-based finite element model

Manufacturing-induced effects significantly affect in-service behaviour of welded structures, such as integrally stiffened panels for aeronautic applications. Being a complex phenomenon with several variables involved, the assessment of the effects coming from welding usually relies on numerical simulations. Here, a novel shell-based finite element model is proposed to accurately simulate the transient thermal fields and stress-strain distributions resulting from friction stir welding (FSW) processes. The capability of the model to predict (i) residual stresses, (ii) material softening and (iii) geometric distortion of the welded parts is assessed by the modelling and simulation of FSW applied on aluminium integrally stiffened panels

Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-drying

Aims: The aim of this study was to optimize survival of Lactobacillus delbrueckii subsp. bulgaricus during spraydrying and subsequent storage through optimizing the pH of growth conditions. Methods and Results: Cell concentrates previously grown without or with pH controlled were spray-dried and stored at 20ºC and heat treated at 57ºC. Cells grown under noncontrolled pH were more resistant to both drying and heating than cells grown under controlled pH but no significant differences were observed during storage. The intracellular proteins profile of cells grown under both conditions was studied by two-dimensional SDS-polyacrylamide gel electrophoresis. Eight proteins were identified using automated mass spectrometry (MS) and tandem mass spectrometry (MS/MS) data acquisition. Of the identified proteins, only cochaperonin GroES corresponded to a known heat shock protein (HSP). The other proteins identified are proteins involved in glycolysis. For cells grown under noncontrolled pH the expression of the Hsp70, GroES and GroEL, measured by Western blotting, was enhanced. Conclusions: The higher resistance of cells grown under noncontrolled pH correlates with the enhanced production of heat shock proteins. Significance and Impact of the Study: Growth of L. bulgaricus under controlled pH (commonly used by the starter cultures production industry) results in cells more sensitive to stresses frequently encountered by the cells during starter cultures preparation/storage/utilization