Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties

Are great tits (Parus major) inhabiting the vicinity of a pulp mill healthy? Impacts on physiology and breeding performance

The evaluation of pollution effects on wildlife provides biologically meaningful information concerning environmental quality and possible impacts on populations and can be used as an early warning of environmental change. We aimed to assess the potential effects of exposure to atmospheric pollution from pulp mill emissions on the morphology, physiology, and reproductive performance of the great tit (Parus major). Great tits from a population inhabiting the vicinity of a pulp mill had significantly higher feather mercury levels and were physiologically distinct from other birds not under the direct influence of the pollution source. This distinction, due to significantly higher levels of red blood cell-glutathione peroxidase antioxidant activity, in birds from the vicinity of the pulp mill, was observed both in autumn and winter and was repeatable between years. No detectable effects were observed on breeding performance or nestling morphology and physiology. The effects of pollution in this study seem to be related to increased levels of oxidative stress.publishe