Sistem Informasi Penyusutan Aset Tetap Peralatan pada Kospin Jasa Cabang di Bandar Lampung

Aset tetap adalah aset berwujud yang diperoleh dalam bentuk siap pakai atau dengan dibangun terlebih dahulu, yang digunakan dalam operasi Perusahaan, tidak dimaksudkan untuk dijual dalam rangka kegiatan Perusahaan dan mempunyai masa manfaat lebih dari satu tahun. Permasalahan yang dihadapi Koperasi Simpan Pinjam Jasa Cabang Bandar Lampung dalam mengolah data aset tetap adalah masih dilakukannya perhitungan secara manual, belum adanya format laporan yang baku, dan sering terjadi kesalahan dalam pelaporan perhitungan aset tetap peralatan dan penyusutannya. Adapun tujuan dari penelitian ini adalah untuk menghasilkan aplikasi aset tetap dalam mengelola peralatan sehingga dapat menghasilkan laporan yang akurat, relavan, dan tepat waktu. Jenis metode penelitian yang digunakan dalam penelitian ini terbagi menjadi Sumber Data Primer, yaitu Observasi, Wawancara, Dokumentasi, Studi Pustaka, dan Sumber Data Sekunder, yaitu Waterfall meliputi Analisa Kebutuhan Sistem, Desain, Coding, Testing, Maintenance. Dengan adanya aplikasi Aset Tetap yang dibuat menggunakan aplikasi NetBeans dan bahasa pemrograman Java, dapat menghasilkan perhitungan, informasi serta laporan yang cepat dan akurat. Sehingga kesalahan dalam pencatatan nama dan nominal aset tetap peralatan dan penyusutannya dapat diminimalisir

Characterization of GPX1 and DIO1 Genes in Bubalus Bubalis

Selenoprotein genes contain selenium in the form of selenocysteine which is involved in protecting the cells from oxidative stress. Soils in India differ greatly in selenium concentrations affecting feed stuffs for selenium availability. Selenoproteins have recently been identified in variety of living organisms including humans which have 25 selenoprotein genes. Among these families of selenoprotein genes, we sequenced Gpx1 gene (Glutathione peroxidases1) and Dio1 gene (Iodothyronine deiodinases) in Bubalus bubalis. Gpx1 is most abundant and ubiquitously expressed selenoprotein which helps to protect against the damaging effects of hydrogen peroxide and oxygen rich free radicals whereas Dio1 is expressed mainly in liver, thyroid gland and adipose tissue, its main function is to convert tetraiodothyronine (T4) to its active form thyroxine (T3) in the presence of deiodinases enzyme. The main aim of the study was to characterize these two genes and to find out the buffalo specific SNPs. This was accomplished by designing primers using cattle database and sequencing a panel of 24 samples consisting of 6 diverse breeds of buffalo. Gpx1 consisted of 2 exons (Accession ID: JQ031269) while Dio1 comprised 4 exons (Accession ID: JQ791197). In Gpx1 gene, 9 SNPs were recorded and 4 were non synonymous, changing amino acid were distributed equally in both exon. In exon 1, A141G (aa Q5R) and G161A (aa A12T); and in exon 2 C785T (aa R132W) and A808T (aa S139R). In Dio1 gene, 3 non synonymous SNPs were identified at A188G (aa H22R), C215G (aa T31R) and G941A (aa V146I). These SNPs are novel and reported for the first time in Indian buffalo and has a potential for their use in diversity analysis and association with various selenium related traits

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S Ahlawat, R Sharma, A.Maitra, M. Roy, M.S. Tantia (2014) Designing, optimization and validation of tetra-primer ARMS PCR protocol for genotyping mutations in caprine Fec Genes. Meta Gene 2 (2014) : 439 - 449.Not AvailableNot Availabl

First-Time Characterization of JY-1-Like Sequence in Goats

The JY-1 protein is oocyte specific, and is associated with folliculogenesis and early embryo development, and thus influences the chance of pregnancy. It was the first protein of maternal origin to be described for a single ovulating species, namely cattle. JY-1-like sequences corresponding to 3’ coding and the untranslated region have been reported in other vertebrate species. This is the first description of the partial JY-1 (exon 3 and 3’UTR) in a livestock species other than cattle and buffalo. The sequence was characterized in a panel of nine Indian goat breeds, which differ in reproductive traits (twinning percentage and age of sexual maturity). Forty three variations were recorded in the analysed region of goats JY-1 compared with cattle. Nucleotide variations in the codifying region of goats correspond to seven amino acid changes that could affect the biological function of the protein and possibly reproductive differences between goats (higher proportion of multiparous animals) and cattle (mainly uniparous). A novel single nucleotide polymorphism (C15329T) has been identified in Indian goats, which was genotyped in 272 animals from six breeds. Further studies to investigate other regions of the gene and its expression in goat female reproductive tissues would clarify the role of JY-1 in farm animal species that are not primarily uniparous.Keywords: Capra Hircus, Embryogenesis, Fertility, Folliculogenesis, Oocyte, PCR-RFLP, Polymorphism, SN

Breed Descriptor of Mewari Camel (Camelus dromedarius)

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