Transthyretin levels in the vitreous correlate with change in visual acuity after vitrectomy

Background/aim: Little is known about biochemical markers related to change in visual acuity after vitrectomy. The potential use of transthyretin (TTR), a carrier of the retinol/retinol-binding protein, as a biochemical marker protein, was investigated. Methods: TTR was measured using immunonephelometry in a group of patients (n = 77) in longstanding (> 1 week) retinal detachment (n = 29), fresh (< 1 week) retinal detachment (n = 17), macular holes (n = 20) or diabetic retinopathy (n = 11). Vitreous samples were taken at the start of every vitrectomy procedure. For reference values, cadaver specimens (n = 73) were used. Results: Reference values for vitreous TTR (median 18 mg/l; IQR 4 to 24 mg/l) comprised 2.2% of reference values for vitreous protein levels (median 538 mg/l; IQR 269 to 987 mg/l). Vitreous TTR values of patients were comparable in all disorders. Vitreous TTR values were higher in phakic (median 22.5 mg/l; IQR 10 to 27 mg/l) than in pseudophakic patients (median 12 mg/l; IQR 8 to 19 mg/l; p = 0.06). Postoperative change in visual acuity correlated well with vitreous TTR values found peroperatively (r(s) = 0.408; p = 0.012). Both change in visual acuity and lens status were the only variables which proved to explain the variance of TTR (multiple correlation coefficient: 0.494; phakic status: t = 2.767; p = 0.0084; and change in visual acuity t = 2.924: p = 0.0056). Conclusion: Vitreous fluid concentrations of TTR can be regarded as a biochemical marker for retinal function

Seeing what teachers see: Exploring the use of eye tracking in teacher expertise studies

Wolff, C., Van ’T Zelfde, H., Jarodzka, H., & Boshuizen, H. P. A. (2012, August). Seeing what teachers see: Exploring the use of eye tracking in teacher expertise studies. Poster presented at the EARLI SIG Learning and Professional Development, Antwerp, Belgium.The exploratory study presented in the poster is part of a larger research project investigating teachers’ cognitive processing and knowledge structuring and how these are expressed at varying levels of expertise development. The experiment is guided by an interest in determining which processes underlie expert teachers’ ability to deal with complex classroom settings and which research methods support such inquiries. As questions about cognitive and perceptual processes are difficult to investigate with traditional methods, we propose the use of process-tracing methods to capture and investigate these processes. Verbalization techniques will be employed to uncover teachers’ cognitive processes. However, as teaching is a highly visual task which places demands on teachers’ visual attention, eye-tracking is proposed as a method for capturing the perceptual and attentional processes of teachers. Eye tracking is a method which allows for the measurement and analysis of eye activity. Eye movements are recorded, measured, and analyzed in relation to the environment in which they occur to infer information about where a person’s gaze (and attention) is directed, the gaze duration, and the order in which gaze (and attention) is allocated. Eye tracking technology currently offers a number of options for collecting eye tracking data. This study considers two systems: a mobile, head-mounted system and a stationary, remote system. These systems were compared to determine the extent to which each system is able to capture covert cognitive and perceptual processes as well as overt behaviour of teachers. The mobile eye tracker is employed directly in the classroom while the stationary eye tracker is applied to a video-taped classroom lesson. The study also explores the effectiveness of eye tracking in combination with other methods, namely think aloud and questionnaires, to detect differences in the skills and knowledge expressed by experienced mentor teachers and inexperienced student teachers

Effect of medical interventions on gender dysphoria and body image : a follow-up study.

Objective: The aim of this study from the European Network for the Investigation of Gender Incongruence is to investigate the status of all individuals who had applied for gender confirming interventions from 2007 to 2009, irrespective of whether they received treatment. The current article describes the study protocol, the effect of medical treatment on gender dysphoria and body image, and the predictive value of (pre) treatment factors on posttreatment outcomes. Methods: Data were collected on medical interventions, transition status, gender dysphoria (Utrecht Gender Dysphoria Scale), and body image (Body Image Scale for transsexuals). In total, 201 people participated in the study (37% of the original cohort). Results: At follow-up, 29 participants (14%) did not receive medical interventions, 36 hormones only (18%), and 136 hormones and surgery (68%). Most transwomen had undergone genital surgery, and most transmen chest surgery. Overall, the levels of gender dysphoria and body dissatisfaction were significantly lower at follow-up compared with clinical entry. Satisfaction with therapy responsive and unresponsive body characteristics both improved. High dissatisfaction at admission and lower psychological functioning at follow-up were associated with persistent body dissatisfaction. Conclusions: Hormone-based interventions and surgery were followed by improvements in body satisfaction. The level of psychological symptoms and the degree of body satisfaction at baseline were significantly associated with body satisfaction at follow-up

Circumstellar disks in binary star systems

In this paper we study the evolution of viscous and radiative circumstellar disks under the influence of a companion star. We focus on the eccentric {\gamma} Cephei and {\alpha} Centauri system as examples and compare the disk quantities such as disk eccentricity and precession rate to previous isothermal simulations. We perform two-dimensional hydrodynamical simulations of the binary star systems under the assumption of coplanarity of the disk, host star and binary companion. We use the grid-based, staggered mesh code FARGO with an additional energy equation to which we added radiative cooling based on opacity tables. The eccentric binary companion perturbs the disk around the primary star periodically. Upon passing periastron spirals arms are induced that wind from the outer disk towards the star. In isothermal simulations this results in disk eccentricities up to {\epsilon}_disk ~ 0.2, but in more realistic radiative models we obtain much smaller eccentricities of about {\epsilon}_disk ~ 0.04 - 0.06 with no real precession. Models with varying viscosity and disk mass indicate show that disks with less mass have lower temperatures and higher disk eccentricity. The rather large high disk eccentricities, as indicated in previous isothermal disk simulations, implied a more difficult planet formation in the {\gamma} Cephei system due to the enhanced collision velocities of planetesimals. We have shown that under more realistic conditions with radiative cooling the disk become less eccentric and thus planet formation may be made easier. However, we estimate that the viscosity in the disk has to very small, with {\alpha} \lesssim 0.001, because otherwise the disk's lifetime will be too short to allow planet formation to occur along the core instability scenario. We estimate that the periodic heating of the disk in eccentric binaries will be observable in the mid-IR regime.Comment: 12 pages, 15 figures, accepted for publication in A&

Mixing of magnetic and phononic excitations in incommensurate Spin-Peierls systems

We analyze the excitation spectra of a spin-phonon coupled chain in the presence of a soliton. This is taken as a microscopic model of a Spin-Peierls material placed in a high magnetic field. We show, by using a semiclassical approximation in the bosonized representation of the spins that a trapped magnetic state obtained in the adiabatic approximation is destroyed by dynamical phonons. Low energy states are phonons trapped by the soliton. When the magnetic gap is smaller than the phonon frequencies the only low energy state is a mixed magneto-phonon state with the energy of the gap. We emphasize that our results are relevant for the Raman spectra of the inorganic Spin-Peierls material CuGeO$_3$.Comment: 5 pages, latex, 2 figures embedded in the tex

KAI407, a potent non-8-aminoquinoline compound that kills Plasmodium cynomolgi early dormant liver stage parasites in vitro.

Preventing relapses of Plasmodium vivax malaria through a radical cure depends on use of the 8-aminoquinoline primaquine, which is associated with safety and compliance issues. For future malaria eradication strategies, new, safer radical curative compounds that efficiently kill dormant liver stages (hypnozoites) will be essential. A new compound with potential radical cure activity was identified using a low-throughput assay of in vitro-cultured hypnozoite forms of Plasmodium cynomolgi (an excellent and accessible model for Plasmodium vivax). In this assay, primary rhesus hepatocytes are infected with P. cynomolgi sporozoites, and exoerythrocytic development is monitored in the presence of compounds. Liver stage cultures are fixed after 6 days and stained with anti-Hsp70 antibodies, and the relative proportions of small (hypnozoite) and large (schizont) forms relative to the untreated controls are determined. This assay was used to screen a series of 18 known antimalarials and 14 new non-8-aminoquinolines (preselected for blood and/or liver stage activity) in three-point 10-fold dilutions (0.1, 1, and 10 μM final concentrations). A novel compound, designated KAI407 showed an activity profile similar to that of primaquine (PQ), efficiently killing the earliest stages of the parasites that become either primary hepatic schizonts or hypnozoites (50% inhibitory concentration [IC50] for hypnozoites, KAI407, 0.69 μM, and PQ, 0.84 μM; for developing liver stages, KAI407, 0.64 μM, and PQ, 0.37 μM). When given as causal prophylaxis, a single oral dose of 100 mg/kg of body weight prevented blood stage parasitemia in mice. From these results, we conclude that KAI407 may represent a new compound class for P. vivax malaria prophylaxis and potentially a radical cure

Native Speaker Perceptions of Accented Speech: The English Pronunciation of Macedonian EFL Learners

The paper reports on the results of a study that aimed to describe the vocalic and consonantal features of the English pronunciation of Macedonian EFL learners as perceived by native speakers of English and to find out whether native speakers who speak different standard variants of English perceive the same segments as non-native. A specially designed computer web application was employed to gather two types of data: a) quantitative (frequency of segment variables and global foreign accent ratings on a 5-point scale), and b) qualitative (open-ended questions). The result analysis points out to three most frequent markers of foreign accent in the English speech of Macedonian EFL learners: final obstruent devoicing, vowel shortening and substitution of English dental fricatives with Macedonian dental plosives. It also reflects additional phonetic aspects poorly explained in the available reference literature such as allophonic distributional differences between the two languages and intonational mismatch