Transformation of 2,4, 6-Trinitrotoluene (TNT) by Immobilized and Resting Cells of Arthrobacter SP.

Arthrobacter sp. transformed 2,4,6-trinitrotoluene (TNT) into two isomeric monoaminodinitrotoluenes viz, 4-amino-2,6-dinitrotoluene (4-ADNT), 2-amino- 4,6 –dinitrotoluene (2-ADNT) and also 2,4- diamino-6- nitrotoluene (2,4-DANT) under aerobic resting cell conditions. Experiments were carried out at 30° C (±1°C) in phosphate buffer with 60 mg per liter TNT at a pH of 7.2 (±0.2). Complete transformation of TNT occurred within 36 hours, yielding a mixture of monoaminodinitrotoluenes and diaminonitrotoluene. The major component was found to be 4-ADNT. Under the same experimental conditions, transformations were studied using barium alginate immobilized cells of Arthrobacter sp. Appropriate controls were run simultaneously. In both the cases, reactions were monitored every six hours by thin-layer chromatography (TLC) and gas chromatography (GC). The products were identified by gas chromatography coupled with mass spectrometry (GC-MS). Immobilization of Arthrobacter sp. in barium alginate resulted in more efficient TNT transformations, which were complete in 24 hours. The immobilized cells could be utilized at least for eight cycles and a decrease in rate of transformation was observed with each cycle

Challenges Preventing Effective Supervision of Universal Basic Education in Kuje Area Council of Federal Capital Territory, Abuja, Nigeria

This study investigated the challenges preventing effective supervision of universal basic education programme in Kuje Area Council of FCT, Abuja, Nigeria. Survey research design was adopted for the study. The population of this study consisted of all the 50 teachers (male and female) in five (5) selected public primary schools and ten educational Supervisors in Kuje Area Council of FCT. Fifth (50) teachers and ten (10) supervisors were selected using purposive sampling technique, which gave a total of 60 respondents as sample size for the study. The Questionnaire used for the study had two sections. Section (A) collected information on bio-data while Section (B) collected information on the subject matter. The teachers’ and supervisors questionnaire which was tagged (Challenges facing the supervision of universal basic school questionnaire) was designed by the researchers and used for data collection (CFSUBEQ). The data collected were analyzed using descriptive statistics (simple percentages). The result produced that inadequate Supervisors, inadequate supervision materials, insecurity, logistics problem, inadequate funding and poor capacity development of supervisors are the challenges preventing effective supervision of universal basic education programme in Kuje Area Council of FCT. Based on this findings, it was recommended that the government should increase the funding of supervision in the Area Council and in the country at large

URBAN FLOOD IMPACTS, FLOOD WATER QUALITY AND RISK MAPPING OF OLODO AREA, IBADAN, NIGERIA

This study assessed urban flood impact, flood water quality and vulnerability around Olodo area of Ibadan region, Nigeria. The study employed remote sensing and GIS techniques in creating vulnerability and risk maps. Digital terrain model (DTM) was used to get the topography of the study area. Footprints of buildings along the Egberi riverbank and flood plain in Olodo were created in the GIS environment from high resolution satellite imagery. Buffering operation was conducted to classify the buildings into risk zones based on closeness to the riverbank using ArcGIS 10.0. The study revealed that 326 buildings were within the very vulnerable and vulnerable zones because they were less than 15.2m away from the riverbank. The characteristics of water quality change during the flood and non-flood periods. TSS, DO, NOD, and COD were all higher during the flood event. Microbial analysis showed that water quality levels in the floodwater exceeded water quality standards (e.g., the coliform excess from 10 to 10,000 times), and thus this may be a health risk for local people during flood events. Concentration of Escherichia coli (E. coli) ranged from 484 to 1290 cfu/100 mL during flooding compared to 192 to 295 cfu/100 mL after flood. Salmonella was found to be high ranging from 659 to 1840 cfu/100 mL during flooding compared to 530 to 1034 cfu/100 mL after flooding.     &nbsp

PIG—the pathogen interaction gateway

Protein–protein interactions (PPIs) play a vital role in initiating infection in a number of pathogens. Identifying which interactions allow a pathogen to infect its host can help us to understand methods of pathogenesis and provide potential targets for therapeutics. Public resources for studying host–pathogen systems, in particular PPIs, are scarce. To facilitate the study of host–pathogen PPIs, we have collected and integrated host–pathogen PPI (HP–PPI) data from a number of public resources to create the Pathogen Interaction Gateway (PIG). PIG provides a text based search and a BLAST interface for searching the HP–PPI data. Each entry in PIG includes information such as the functional annotations and the domains present in the interacting proteins. PIG provides links to external databases to allow for easy navigation among the various websites. Additionally, PIG includes a tool for visualizing a single HP–PPI network or two HP–PPI networks. PIG can be accessed at http://pig.vbi.vt.edu

A system to test the effects of materials on the electron drift lifetime in liquid argon and observations on the effect of water

A materials test system (MTS) has been developed at FNAL to assess the suitability of materials for use in a large liquid argon time projection chamber. During development of the MTS, it was noted that controlling the cryostat pressure with a 'raining' condenser reduced the electron drift lifetime in the liquid argon. The effect of condensing has been investigated using a series of passive materials to filter the condensate. We report the results of these studies and of tests on different candidate materials for detector construction. The inferred reduction of electron drift lifetime by water concentrations in the parts per trillion is of particular interest

A Regenerable Filter for Liquid Argon Purification

A filter system for removing electronegative impurities from liquid argon is described. The active components of the filter are adsorbing molecular sieve and activated-copper-coated alumina granules. The system is capable of purifying liquid argon to an oxygen-equivalent impurity concentration of better than 30 parts per trillion, corresponding to an electron drift lifetime of at least 10 ms. Reduction reactions that occur at about 250 degrees Celsius allow the filter material to be regenerated in-situ through a simple procedure. In the following work we describe the filter design, performance, and regeneration process.Comment: 12 pages with 9 figure

Electrochemical control of high carbon steel corrosion using rosemary oil in citric acid medium

The Inhibition efficiency of Rosemary oil (Rosmarinus officinalis) for corrosion control of high carbon steel was done in citric acid medium by weight loss method. The Rosmarinus officinalis was administered in the proportion of 0%, 0.50%, 1.00%, 1.50%, 2.00% and 2.50% concentration. This study unveiled that the inhibition efficiency performed excellently with maximum percentage of 88.3% at room temperature in 0.5M citric acid solution. This has shown the effectiveness of Rosmarinus officinalis inhibitor in corrosion analysis of high carbon steel