EuroFlow Standardized Approach to Diagnostic Immunopheneotyping of Severe PID in Newborns and Young Children

The EuroFlow PID consortium developed a set of flow cytometry tests for evaluation of patients with suspicion of primary immunodeficiency (PID). In this technical report we evaluate the performance of the SCID-RTE tube that explores the presence of recent thymic emigrants (RTE) together with T-cell activation status and maturation stages and discuss its applicability in the context of the broader EuroFlow PID flow cytometry testing algorithm for diagnostic orientation of PID of the lymphoid system. We have analyzed peripheral blood cells of 26 patients diagnosed between birth and 2 years of age with a genetically defined primary immunodeficiency disorder: 1

Quality control and quantification in IG/TR next-generation sequencing marker identification: protocols and bioinformatic functionalities by EuroClonality-NGS

Assessment of clonality, marker identification and measurement of minimal residual disease (MRD) of immunoglobulin (IG) and T cell receptor (TR) gene rearrangements in lymphoid neoplasms using next-generation sequencing (NGS) is currently under intensive development for use in clinical diagnostics. So far, however, there is a lack of suitable quality control (QC) options with regard to standardisation and quality metrics to ensure robust clinical application of such approaches. The EuroClonality-NGS Working Group has therefore established two types of QCs to accompany the NGS-based IG/TR assays. First, a central polytarget QC (cPT-QC) is used to monitor the primer performance of each of the EuroClonality multiplex NGS assays; second, a standardised human cell line-based DNA control is spiked into each patient DNA sample to work as a central in-tube QC and calibrator for MRD quantification (cIT-QC). Having integrated those two reference standards in the ARResT/Interrogate bioinformatic platform, EuroClonality-NGS provides a complete protocol for standardised IG/TR gene rearrangement analysis by NGS with high reproducibility, accuracy and precision for valid marker identification and quantification in diagnostics of lymphoid malignancies.This work was supported by Ministry of Health of the Czech Republic, grant no. 16-34272A; computational resources were provided by the CESNET LM2015042 and the CERIT Scientific Cloud LM2015085, provided under the programme “Projects of Large Research, Development, and Innovations Infrastructures”. Analyses in Prague (JT, EF and MS) were supported by Ministry of Health, Czech Republic, grant no. 00064203, and by PRIMUS/17/MED/11. Analyses in the Monza (Centro Ricerca Tettamanti, SS, AG and GC) laboratory were supported by the Italian Association for Cancer Research (AIRC) and Comitato Maria Letizia Verga

Challenges in numerical simulation of electromagnetic material evaluation processes for ferromagnetic specimens

Conference of 54th Annual British Conference of Non-Destructive Testing, NDT 2015 ; Conference Date: 8 September 2015 Through 10 September 2015; Conference Code:116980International audienceThe numerical solution of the non-linear, hysteretic electromagnetic induction problem involved in material evaluation techniques is discussed. The general solution scheme for this kind of problems is presented, and emphasis is given at those specific points, which are critical for the solution accuracy and the performance of the solver. For the purposes of this study, two representative cases, frequently met in material evaluation applications, namely the inspection of a planar ferromagnetic specimen via a magnetic yoke and a set of air-coils, respectively, are treated using two numerical solvers based on the Finite Integration Technique (FIT) and the Finite Elements Technique (FEM). The results obtained with these two methods and the respective performances of the solvers are discussed

Impact of the hysteresis description model on the numerical simulation results for yoke-oriented inspection configurations

International audienceA 2D numerical model for the solution of the eddy-current induction problem in non-linear hysteretic media is presented. The convergence of the iterative algorithm for the inversion of the non-linear operator is discussed. The numerical results are compared with simulations carried our using a commercial finite elements solver. Finally, an experimental procedure for obtaining the input material curves is presented