SNS Timing System

This poster describes the timing system being designed for Spallation Neutron Source being built at Oak Ridge National lab

The aromatic amino acid hydroxylase genes AAH1 and AAH2 in Toxoplasma gondii contribute to transmission in the cat

The Toxoplasma gondii genome contains two aromatic amino acid hydroxylase genes, AAH1 and AAH2 encode proteins that produce L-DOPA, which can serve as a precursor of catecholamine neurotransmitters. It has been suggested that this pathway elevates host dopamine levels thus making infected rodents less fearful of their definitive Felidae hosts. However, L-DOPA is also a structural precursor of melanins, secondary quinones, and dityrosine protein crosslinks, which are produced by many species. For example, dityrosine crosslinks are abundant in the oocyst walls of Eimeria and T. gondii, although their structural role has not been demonstrated, Here, we investigated the biology of AAH knockout parasites in the sexual reproductive cycle within cats. We found that ablation of the AAH genes resulted in reduced infection in the cat, lower oocyst yields, and decreased rates of sporulation. Our findings suggest that the AAH genes play a predominant role during infection in the gut of the definitive feline host

Optical fibre digital pulse-position-modulation assuming a Gaussian received pulse shape

The abundance in bandwidth available in the best monomode fibres may be exchanged for improved receiver sensitivity by employing digital PPM. The paper presents a performance and optimisation analysis for a digital PPM coding scheme operating over a fibre channel employing a PIN-BJT receiver and assuming a Gaussian received pulse shape. The authors present original results for a 50 Mbit/s, 1.3 μm wavelength digital PPM system and conclude that, provided the fibre bandwidth is several times that of the data rate, digital PPM can outperform commercially available PIN-BJT binary PCM system

Field-scale validation of an automated soil nitrate extraction and measurement system

One of the many gaps that needs to be solved by precision agriculture technologies is the availability of an economic, automated, on-the-go mapping system that can be used to obtain intensive and accurate ‘real-time’ data on the levels of nitrate nitrogen (NO3–N) in the soil. A soil nitrate mapping system (SNMS) has been developed to provide a way to collect such data. This study was done to provide extensive field-scale validation testing of the system’s nitrate extraction and measurement sub-unit (NEMS) in two crop (wheat and carrot) production systems. Field conditions included conventional tillage (CT) versus no tillage (NT), inorganic versus organic fertilizer application, four soil groups and three points in time throughout the season. Detailed data analysis showed that: (i) the level of agreement, as measured by root mean squared error (RMSE), mean absolute error (MAE) and coefficient of efficiency (CE), between NEMS soil NO3–N and standard laboratory soil NO3–N measurements was excellent; (ii) at the field-scale, there was little practical difference when using either integer or real number data processing; (iii) regression equations can be used to enable field measurements of soil NO3–N using the NEMS to be obtained with laboratory accuracy; (iv) future designs of the SNMS’s control system can continue to use cheaper integer chip technology for processing the nitrate ion-selective electrode (NO3 -–ISE) readings; and (v) future designs of the SNMS would not need a soil moisture sensor, ultimately saving on manufacturing costs of a more simple syste

Video-Based Analyses of Parkinson's Disease Severity: A Brief Review

Remote and objective assessment of the motor symptoms of Parkinson's disease is an area of great interest particularly since the COVID-19 crisis emerged. In this paper, we focus on a) the challenges of assessing motor severity via videos and b) the use of emerging video-based Artificial Intelligence (AI)/Machine Learning techniques to quantitate human movement and its potential utility in assessing motor severity in patients with Parkinson's disease. While we conclude that video-based assessment may be an accessible and useful way of monitoring motor severity of Parkinson's disease, the potential of video-based AI to diagnose and quantify disease severity in the clinical context is dependent on research with large, diverse samples, and further validation using carefully considered performance standards

Mobilizing Cause Supporters through Group-Based Interaction

This is the author accepted manuscript. The final version is available from Wiley via the DOI in this record.Collective action expresses group-based identities, formed by supporters seeking to further particular social causes. While the development of groups linked to action necessitates interaction among supporters, little research has examined how these groups form. Utilizing responses of supporters who participated in 1 of 29 action-planning sessions, this research presents an initial attempt to identify the ingredients important to this process. It shows that to the extent that the actions agreed on in the course of group interactions were seen as capable of making a difference (action efficacy), and worthy of public expression (action voice), supporters’ group-based identification was enhanced. This in turn increased their willingness to engage in collective action. Practical implications and avenues for future research to understand the mobilization process are discussed.Australian Research Counci

Reassessment of the role of aromatic amino acid hydroxylases and the effect of infection by Toxoplasma gondii on host dopamine

Toxoplasma gondii infection has been described previously to cause infected mice to lose their fear of cat urine. This behavioral manipulation has been proposed to involve alterations of host dopamine pathways due to parasite-encoded aromatic amino acid hydroxylases. Here, we report successful knockout and complementation of the aromatic amino acid hydroxylase AAH2 gene, with no observable phenotype in parasite growth or differentiation in vitro and in vivo. Additionally, expression levels of the two aromatic amino acid hydroxylases were negligible both in tachyzoites and in bradyzoites. Finally, we were unable to confirm previously described effects of parasite infection on host dopamine either in vitro or in vivo, even when AAH2 was overexpressed using the BAG1 promoter. Together, these data indicate that AAH enzymes in the parasite do not cause global or regional alterations of dopamine in the host brain, although they may affect this pathway locally. Additionally, our findings suggest alternative roles for the AHH enzymes in T. gondii, since AAH1 is essential for growth in nondopaminergic cells

Divergence of the single-copy DNA sequences of the Western Grebe (Aechmophorus occidentalis) and Clark’s Grebe (A. clarkii), as indicated by DNA-DNA hybridization

Single-copy nuclear DNA sequences of individuals of Aechmophorus occidentalis and A. ciarkii were compared by DNA-DNA hybridization. In each of three experimental sets the average thermal stability of homoduplex and within-species DNA-DNA hybrids did not differ, but the between-species DNA-DNA hybrids dissociated at an average temperature 0.57°C below the median melting temperature of homoduplex and within-species hybrids. The difference was highly significant in all three sets. The median DNA sequence distance between A. occidentalis and A. clarkii is comparable to such distances between other closely related congeneric species