319 research outputs found

    Strategi Pengembangan Usaha Peternakan Babi Di Desa Tlekung Kecamatan Junrejo Kota Batu

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    Tujuan dari penelitian ini yaitu untuk mengetahui strategi pengembangan usaha Peternakan babi, mengetahui faktor apa yang mempengaruhi perkembangan peternakan babi, mengetahui letak usaha dari strategi usaha ternak babi di Di Desa Tlekung Kecamatan Junrejo Kota Batu. Analisa data menggunakan analisis SWOT. Alternatif strategi menurut output matriks SWOT yaitu, mempertahankan dan meningkatkan interaksi dengan manejer dan karyawan perusahaan, kemitraan, pelanggan dan lembaga memperluas jangkauan pemasaran, meningkatkan dan menjaga kualitas daging babi tersebut. Berdasarkan kajian terhadap peternakan babi di Desa Tlekung Kecamatan Junrejo Kota Batu dapat dikemukakan bahwa: 1. Berdasarkan matriks SWOT, peternakan babi dikembangkan untuk dapat bertahan dan bersaing, menghasilkan laba yang dibutuhkan peternak sebagai pelaku usaha. Rencana pertumbuhan bisnis merupakan ujung tombak untuk menghadapi persaingan saat ini. Desa Tlekung Kecamatan Junrejo Kota Batu Karakteristik pemasaran dan manufaktur menjadi kekuatannya. Kemungkinan untuk mengurangi ancaman adalah bantuan pemerintah untuk pertimbangan penyakit dan teknologi. 2. Strategi yang perlu ditingkatkan untuk pengembangan peternakan babi dan untuk mengetahui posisinya adalah strategi WT (Weakness and Threats), sebagai strategi yang paling dominan berdasarkan aktivitas bertahan, untuk minimalkan kelemahan dan hindari ancaman, sehingga dapat diterapkan pada peternakan babi di Desa Tlekung Kecamatan Junrejo Kota Batu. Peternakan petani kecil biasanya memelihara babi secara individual, yang membatasi akses mereka ke pasar, daya tawar, biaya pemasaran, kemungkinan pasar, keahlian, dan layanan pemerintah

    The Occurrence of CSN1S1 E Allele on Saanen Goat Population at BBPTU-HPT Baturraden

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    The CSN1S1 gene is expressed as the milk alpha S1 casein which is valuable for cheese making. Allele E is a mutation of this gene, which is correlated with low quantity of expressed protein. This study aimed to identify the occurrence of CSN1S1 E allele on the Saanen population at BBPTU-HPT Baturraden. Blood samples of 45 does were drawn, followed by DNA isolation using the column spin method. The identification of E allele was carried out by Allele Specific Polymerase Chain Reactions (AS-PCR) with forward primer 5-TCAGGAGCAGTGGGTATGTG-3 and reverse 5-CCTCCCAATGGAATAATGACA-3. Allele E was found as heterozygote genotype with non-E allele in 28 out of 45 samples. This means that F allele frequency was 0.31 and 0.6 for non-E allele. There was no significant difference of milk protein in both groups. In summary, E allele was found in the Saanen goat population in BBPTU HPT Baturraden with no effect on milk protein production. Keywords: Saanen, CSN1S1, milk, goats, protein

    Studi Komparasi Metode Pembelajaran Kooperatif Numbered Heads Together (Nht) Dan Think Pair Share (Tps) Dengan Memperhatikan Kemampuan Matematika Terhadap Prestasi Belajar Siswa Pada Materi Pokok Larutan Penyangga Di SMA Negeri 1 Jumapolo

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    Tujuan penelitian ini untuk mengetahui: (1) pengaruh metode pembelajaran kooperatif NHT dan TPS terhadap prestasi belajar siswa pada materi pokok Larutan Penyangga. (2) pengaruh kemampuan matematika terhadap pretasi belajar siswa pada materi pokok Larutan Penyangga. (3) interaksi antara metode pembelajaran kooperatif NHT dan TPS dengan memperhatikan kemampuan matematika terhadap prestasi belajar siswa pada materi pokok Larutan Penyangga. Populasi dalam penelitian ini adalah seluruh siswa kelas XI IPA semester genap SMA Negeri 1 Jumapolo tahun pelajaran 2013/2014. Sampel diambil dengan teknik Cluster Random Sampling sejumlah 2 kelas. Pengumpulan data dilakukan menggunakan teknik tes dan non tes. Teknik tes untuk prestasi kognitif dan kemampuan matematika sedangkan teknik non tes untuk prestasi afektif. Analisis data menggunakan Analisis Variansi dua jalan dengan sel tak sama. Berdasarkan hasil penelitian dapat disimpulkan bahwa: (1) prestasi kognitif siswa pada metode pembelajaran kooperatif NHT lebih tinggi daripada metode pembelajaran kooperatif TPS. Hal ini ditunjukkan dari rerata kelas NHT yaitu 76,36 sedangkan kelas TPS yaitu 60,76. Sedangkan prestasi afektif siswa, pembelajaran kooperatif NHT mempunyai nilai rata-rata lebih tinggi dibandingkan dengan pembelajaran kooperatif TPS, (2) prestasi belajar siswa yang mempunyai kemampuan matematika tinggi lebih baik daripada prestasi belajar siswa yang mempunyai kemampuan matematika rendah, (3) tidak ada interaksi antara metode pembelajaran kooperatif NHT dan TPS dengan kemampuan matematika terhadap prestasi belajar siswa. Hal ini dapat ditunjukkan dari besarnya Fobs (0,131) lebih kecil daripada Ftabel (4,00) pada prestasi kognitif, sedangkan besarnya Fobs (0,029) lebih kecil daripada Ftabel (4,00) pada prestasi afekti

    Utilization of Cpdna Sequences to Identify 15 Mango Accessions

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    Information about genetic relationship and genetic diversity are an inevitable need in mango breeding program. Base substitution value, genetic distance and grouping of 15 mango accessions based on accessions in chloroplast DNA (cpDNA) among 15 mango accessions were assessed. The samples were originated from Mango Germplasm, Cukur Gondang Research Station of Indonesian Tropical Fruits Research Institute, Pasuruan, East Java. Sequencing of Chloroplast DNA was used to obtain nucleotide sequence data. Paired specific primer rpl20 F - rps12 R and atp F - rbcL R were used for amplification of non-coding area of mango DNA chloroplast and sequencing processes as well. All data were analyzed using Software MEGA 6. The result showed that total numbers and nucleotide base sequences varied among all accessions. All accessions were grouped in five different clusters that might be used as source of parental breeding

    Factors Associated with Health Cadre Ability to Promote Health of Pregnant Women in Sleman Regency, Yogyakarta

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    Background: Maternal mortality rate in Indonesia is high, with approximately 305 deaths/100,000 live births. Maternal mortality often occurs because of labor complication. There is a need for improvement in the quality of women health. Health promotion on pregnant women is an effort to increase awareness and ability to control healthy life. Health cadres are expected to improve the quality of maternal health. This study aimed to determine factors associated with health cadre ability to promote the health of pregnant women in Sleman Regency, Yogyakarta. Subjects and Method: This was an analytic quantitative study with a cross-sectional approach, conducted in Sleman Regency, Yogyakarta from February to March 2018. A sample of 269 active cadre was selected for this study by multistage cluster random sampling technique. The dependent variables were cadre attitude, awareness, and ability to promote the health of pregnant women. The independent variables were education, knowledge, experience, and attitude. The data were collected by questionnaire and analyzed by path analysis. Results: Factors influencing cadre attitude were education (b= 0.13; SE= 0.50; p>0.005), knowledge (b= 0.06; SE= 0.13; p>0.005), and experience (b= 0.02; SE= 0.05; p>0.005). Factors influencing cadre awareness and ability to promote health of pregnant women were (b= 0.55; SE= 0.12; p>0.005), knowledge (b= 0.94; SE= 0.28; p<0.001), experience (b= 0.41; SE= 0.10; p<0.001), attitude (b= 0.39; SE= 0.12; p<0.005). Fit model: p= 0.528; GFI= 0.99; NFI=0.99; CFI= 1.00; RMSEA< 0.001. Conclusion: Factors influencing cadre attitude is education, knowledge, and experience. Factors influencing cadre awareness and ability to promote the health of pregnant women are knowledge, experience, and attitude. Keywords: education, knowledge, experience, attitude, health promotion, cadres

    A novel reversed phase high performance liquid chromatography method to accurately determine low concentrations of curcumin in rat plasma

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    Due to its lipophilic nature, curcumin levels in plasma are very low after oral administration, and therefore hard to detect. A number of chromatographic methods, including LC/MS have been developed. Although the LC/MS method is sensitive, the matrix effect can be difficult to handle. Furthermore, LC/MS equipment is relatively expensive compared to the conventional RP-HPLC. Therefore, the aim of this study was to develop a sensitive and reliable method for the determination of curcumin concentration in plasma using an RP-HPLC. Curcuma longa extract was used which contains three curcuminoids. The method started by selection of mobile phase to optimally separate the curcuminoids. The best mobile phase composition was used to analyze the plasma samples. Rat plasma was spiked with curcumin and processed for protein precipitation followed by liquid-liquid extraction of curcuminoids and an internal standard, emodin. Chromatogaphic separation of curcuminoids and emodin was achieved using a Knauer C-18 column (250 x 4.6 mm; particle size: 5µm) and a gradient program of mobile phase of three solvents, methanol -acetonitrile-1% acetic acid. A gradient elution was applied with increasing the ratio of the volume percentages of acetonitrile to acetic acid from 50/45 to 53/42 during 5 minute and thereafter elution was isocratic for 15 minute. The methanol concentration was kept constant at 5 vol-% during the whole run. The method was validated according to the FDA guidelines.The method was selective, with an excellent resolution (Rs value > 2.5). The peak shape of both curcumin and emodin were symmetric with a tailing factor of 0.9-1.1. The method linearity (correlation coefficient of 0.999) was demonstrated at 6 to 200 ng/mL. The intra and inter-day precision was 5.90-8.50% and 5.37-11.26%, respectively; the intra- and inter-day accuracy was 92.47-103.61% and 96.17-105.70%, respectively. In conclusion, the RP-HPLC method meets the validation requirements as described in the FDA guidelines and is applicable to accurately quantify curcumin concentrations as low as 6 ng/mL in rat plasma samples
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