Dynamical systems analysis of spike-adding mechanisms in transient bursts

Transient bursting behaviour of excitable cells, such as neurons, is a common feature observed experimentally, but theoretically, it is not well understood. We analyse a five-dimensional simplified model of after-depolarisation that exhibits transient bursting behaviour when perturbed with a short current injection. Using one-parameter continuation of the perturbed orbit segment formulated as a well-posed boundary value problem, we show that the spike-adding mechanism is a canard-like transition that has a different character from known mechanisms for periodic burst solutions. The biophysical basis of the model gives a natural time-scale separation, which allows us to explain the spike-adding mechanism using geometric singular perturbation theory, but it does not involve actual bifurcations as for periodic bursts. We show that unstable sheets of the critical manifold, formed by saddle equilibria of the system that only exist in a singular limit, are responsible for the spike-adding transition; the transition is organised by the slow flow on the critical manifold near folds of this manifold. Our analysis shows that the orbit segment during the spike-adding transition includes a fast transition between two unstable sheets of the slow manifold that are of saddle type. We also discuss a different parameter regime where the presence of additional saddle equilibria of the full system alters the spike-adding mechanism

Coral calcification under daily oxygen saturation and pH dynamics reveals the important role of oxygen

Coral reefs are essential to many nations, and are currently in global decline. Although climate models predict decreases in seawater pH (~0.3 units) and oxygen saturation (~5 percentage points), these are exceeded by the current daily pH and oxygen fluctuations on many reefs (pH 7.8–8.7 and 27–241% O2 saturation). We investigated the effect of oxygen and pH fluctuations on coral calcification in the laboratory using the model species Acropora millepora. Light calcification rates were greatly enhanced (+178%) by increased seawater pH, but only at normoxia; hyperoxia completely negated this positive effect. Dark calcification rates were significantly inhibited (51–75%) at hypoxia, whereas pH had no effect. Our preliminary results suggest that within the current oxygen and pH range, oxygen has substantial control over coral growth, whereas the role of pH is limited. This has implications for reef formation in this era of rapid climate change, which is accompanied by a decrease in seawater oxygen saturation owing to higher water temperatures and coastal eutrophication

The effect of depth on the morphology, bacterial clearance, and respiration of the mediterranean sponge chondrosia reniformis (Nardo, 1847)

To support the successful application of sponges for water purification and collagen production, we evaluated the effect of depth on sponge morphology, growth, physiology, and functioning. Specimens of Eastern Mediterranean populations of the sponge Chondrosia reniformis (Nardo, 1847) (Demospongiae, Chondrosiida, Chondrosiidae) were reciprocally transplanted between 5 and 20 m depth within the KaŠ-Kekova Marine Reserve Area. Control sponges at 5 m had fewer but larger oscula than their conspecifics at 20 m, and a significant inverse relationship between the osculum density and size was found in C. reniformis specimens growing along a natural depth gradient. Sponges transplanted from 20 to 5 m altered their morphology to match the 5 m control sponges, producing fewer but larger oscula, whereas explants transplanted from 5 to 20 m did not show a reciprocal morphological plasticity. Despite the changes in morphology, the clearance, respiration, and growth rates were comparable among all the experimental groups. This indicates that depth-induced morphological changes do not affect the overall performance of the sponges. Hence, the potential for the growth and bioremediation of C. reniformis in mariculture is not likely to change with varying culture depth. The collagen content, however, was higher in shallow water C. reniformis compared to deeper-growing sponges, which requires further study to optimize collagen production.This research was executed within the Connected Circularity program, financed by strategic funding of Wageningen University and Research and the knowledge base of the Ministry of Agriculture, Nature, and FoodQuality (KB40), and was part of the ERA-NET project Biogenink (project 4195), funded by the EuropeanCommission in conjunction with the Dutch Science Foundation NWO and the Portuguese Foundation for Science and Technology (FCT) (project M-ERA-NET-2/0022/2016)

Overt and subtle discrimination and psychological well-being:Examining the mediating and moderating role of ethnic-racial identity among emerging adults

Ethnic-racial identity (ERI) has been reported as mediator and moderator of the relation between discrimination and psychological well-being. However, it remains unclear how different forms of discrimination (i.e., overt and subtle) predict well-being over time, and whether ERI exploration and commitment mediate or moderate this association. This preregistered study explored the associations of overt and subtle discrimination with well-being (i.e., depression, substance use, life satisfaction) in a sample of 323 ethnic-racial minoritized college students (M age W1 = 18.03, 62.7% female) from longitudinal data collected in the US. Cross-lagged panel models across three waves indicated no associations of overt discrimination, but participants experiencing more subtle discrimination during their transition to college reported more depressive symptoms after four months. Ethnic-racial identity did not function as mediator or moderator. Findings indicate the need for a more nuanced understanding of the role of ERI during emerging adulthood.</p

Cytochrome oxidase subunit VI of Trypanosoma brucei is imported without a cleaved presequence and is developmentally regulated at both RNA and protein levels

Mitochondrial respiration in the African trypanosome undergoes dramatic developmental stage regulation. This requires co-ordinated control of components encoded by both the nuclear genome and the kinetoplast, the unusual mitochondrial genome of these parasites. As a model for understanding the co-ordination of these genomes, we have examined the regulation and mitochondrial import of a nuclear-encoded component of the cytochrome oxidase complex, cytochrome oxidase subunit VI (COXVI). By generating transgenic trypanosomes expressing intact or mutant forms of this protein, we demonstrate that COXVI is not imported using a conventional cleaved presequence and show that sequences at the N-terminus of the protein are necessary for correct mitochondrial sorting. Analyses of endogenous and transgenic COXVI mRNA and protein expression in parasites undergoing developmental stage differentiation demonstrates a temporal order of control involving regulation in the abundance of, first, mRNA and then protein. This represents the first dissection of the regulation and import of a nuclear-encoded protein into the cytochrome oxidase complex in these organisms, which were among the earliest eukaryotes to possess a mitochondrion

The detection of cluster magnetic fields via radio source depolarisation

It has been well established that galaxy clusters have magnetic fields. The exact properties and origin of these magnetic fields are still uncertain even though these fields play a key role in many astrophysical processes. Various attempts have been made to derive the magnetic field strength and structure of nearby galaxy clusters using Faraday rotation of extended cluster radio sources. This approach needs to make various assumptions that could be circumvented when using background radio sources. However, because the number of polarised radio sources behind clusters is low, at the moment such a study can only be done statistically. In this paper, we investigate the depolarisation of radio sources inside and behind clusters in a sample of 124 massive clusters at $z<0.35$ observed with the Karl G. Jansky Very Large Array. We detect a clear depolarisation trend with the cluster impact parameter, with sources at smaller projected distances to the cluster centre showing more depolarisation. By combining the radio observations with ancillary X-ray data from Chandra, we compare the observed depolarisation with expectations from cluster magnetic field models using individual cluster density profiles. The best-fitting models have a central magnetic field strength of $5-10\,\mu$G with power-law indices between $n=1$ and $n=4$. We find no strong difference in the depolarisation trend between sources embedded in clusters and background sources located at similar projected radii, although the central region of clusters is still poorly probed by background sources. We also examine the depolarisation trend as a function of cluster properties such as the dynamical state, mass, and redshift. Our findings show that the statistical depolarisation of radio sources is a good probe of cluster magnetic field parameters. [abridged]Comment: Replaced with Published version (A&A). 32 pages, 34 figure

Immune checkpoint inhibitor-associated myocarditis:Case reports and a review of the literature

Immune checkpoint inhibitors (ICIs) are increasingly recognised to effectuate long-lasting therapeutic responses in solid tumours. However, ICI therapy can also result in various immune-related adverse events, such as ICI-associated myocarditis, a rare but serious complication. The clinical spectrum is wide and includes asymptomatic patients and patients with fulminant heart failure, making it challenging to diagnose this condition. Furthermore, the optimal diagnostic algorithm and treatment of ICI-associated myocarditis is unknown. In this review, we describe two cases on both ends of the spectrum and discuss the challenges in recognising, diagnosing and treating ICI-associated myocarditis