Effects of alpha fetoprotein on escape of Bel 7402 cells from attack of lymphocytes

BACKGROUND: Involvement of AFP against apoptosis of tumor cell has been implicated in its evasion of immune surveillance. However, the molecular events of immune escape mechanisms are still unknown. The major observations reported here relate to a possible mechanism by which heptoloma Bel 7402 cells escape immune surveillance in vitro. METHODS: Western blotting and a well-characterized cofocal scanning image were performed to analyze the expression of Fas/FasL and caspase-3 in co-cultured Bel 7402 and Jurkat cells. RESULTS: After co-culture with Jurkat cells, up-regulated Fas and reduced FasL expression could be observed. Treatment with AFP could remarkably inhibit the elevated Fas and, whereas, induce the FasL expression in co-cultured Bel 7402 cells. Cells co-culture could induce the expression of caspase-3 in both cells line. The elevated caspase-3 in Bel 7402 cells was abolished following the treatment of AFP. The expression of caspase-3 was elevated in co-cultured Jurkat cells treated with AFP. No detectable change on the expression of survivin was examined in both cells line. Monoclonal antibody against AFP treatment alone did not obviously influence the growth of cells, as well as the expression of Fas/FasL and caspase-3. However, the effect of AFP could be blocked by antibody. CONCLUSIONS: our results provide evidence that AFP could promote the escape of liver cancer cells from immune surveillance through blocking the caspase signal pathway of tumor cells and triggering the Fas/FasL interaction between tumor cells and lymphocytes

Сердечный индекс и вариация ударного объема на основе анализа времени транзита пульсовой волны в сравнении с производными анализа контура пульсовой волны после коронарной реваскуляризации на работающем сердце

The objective was to validate cardiac index (CI) and stroke volume variation (SVV) measured by pulse wave transit time (PWTT) technology  using estimated continuous cardiac output (esCCO) technique, with pulse contour analysis (PCA) after off-pump coronary artery bypass grafting  (OPCAB)Materials and methods. The study involved 21 patients after elective OPCAB. In all patients, CI and SVV were measured with both esCCO technique  (CIesCCO and esSVV) and PCA (CIPCA and SVVPCA). The agreement between methods was analyzed using correlation analysis and Bland-Altman  analysis. In addition, the trending ability of esCCO technique to control changes in CI during dynamic tests was investigated.  Results. During the study, 178 pairs for CI and 174 pairs for SVV were collected. The mean bias between CIesCCO and CIPCA was 0.06 L·min–1 m–2 with limits of agreement of ± 0.92 L·min–1 m–2 and a percentage error of 35.3%. The concordance rate of CIesCCO was 70%. The mean bias between  esSVV and SVVPCA achieved – 6.1% with limits of agreement of ± 15.5% and percentage error of 137%.Conclusions. The coherence of CIesCCO and esSVV based on PWTT in comparison with PCA is not appropriate. Further development of this  monitoring algorithm may be required for more correct measurement of cardiac output and fluid responsiveness  Цель – провести валидацию сердечного индекса (СИ) и вариации ударного объема (ВУО), измеренных с помощью метода анализа  времени транзита пульсовой волны (ВТПВ) с использованием технологии estimated continuous cardiac output (esCCO), с показателями, полученными на основе анализа контура пульсовой волны (АКПВ), после аортокоронарного шунтирования на работающем  сердце (АКШ).Материалы и методы. В исследование был включен 21 пациент после планового АКШ. Всем пациентам была выполнена оценка СИ и ВУО  как с помощью технологии ВТПВ (СИВТПВ и ВУОВТПВ), так и на основе АКПВ (СИАКПВ и ВУОАКПВ). Согласованность между методами была  оценена с помощью корреляционного анализа и анализа Бланда – Альтмана. Кроме того, была произведена оценка способности технологии  esCCO контролировать изменения СИ на фоне динамических тестов.Результаты. В ходе исследования было получено 178 пар данных для СИ и 174 пары данных для ВУО. Средняя разница между СИВТПВ  и СИАКПВ составила 0,06 л∙мин–1∙м–2 с границей согласованности ± 0,92 л∙мин–1∙м–2 и процентной ошибкой 35,3%. Показатель конкордантности  для СИВТПВ составил 70%. Средняя разница между ВУОВТПВ и ВУОАКПВ достигла 6,1% с пределом согласованности ± 15,5% и процентной  ошибкой 137%. Заключение. Показатели СИ и ВУО, полученные с помощью анализа ВТПВ, обладают недостаточной согласованностью в сравнении c  АКПВ. Необходимо дальнейшее совершенствование данного алгоритма мониторинга для более точной оценки сердечного выброса и восприимчивости к инфузионной нагрузке.

The Apoptosome: Emerging Insights and New Potential Targets for Drug Design

Apoptosis plays a crucial role in tissue homeostasis, development and many diseases. The relevance of Apaf1, the molecular core of apoptosome, has been underlined in mitochondria-dependent apoptosis, which according to a growing body of evidence, is involved in various pathologies where the equilibrium of life-and-death is dysregulated, such as heart attack, stroke, liver failure, cancer and autoimmune diseases. Consequently, great interest has emerged in devising therapeutic strategies for regulating the key molecules involved in the life-and-death decision. Here we review recent progress in apoptosis-based pharmacological therapies and, in particular, we point out a possible role of the apoptosome as an emerging and promising pharmacological target

Sex steroids and oocyte maturation in the sterlet (Acipenser ruthenus L.)

Sturgeon oocytes, like those of other vertebrates, are arrested in early meiosis and require a steroid or steroids to trigger meiosis resumption or maturation. The identity of the maturation-inducing steroid in sturgeons has present study was designed to investigate in sterlet the follicular secretion and relative blood changes of free and conjugated 17,20βP, 20βS and of the androgens T, 11KT, in response to hormonal treatment and to determine the relative potencies of the progestagens, 17,20β-P, 20βS, P4 and S, in inducing oocyte final maturation in vitro

Computer science. Иностранный язык

Учебно-методическое пособие.Доступ к полному тексту открыт из сети СФУ, вне сети доступ возможен для читателей Научной библиотеки СФУ или за плату.Учебно-методическое пособие включает в себя тексты по специальности и ряд упражнений грамматического и лексического характера. Предназначено для аудиторной и самостоятельной работы студентов второго курса направления 15.03.05 «Конструкторско-технологическое обеспечение машиностроительных производств»

Monitoring of Blood Phagocyte Functional State by Cyclic Dielectric Spectroscopy

The study of functional state of blood phagocytes adhering to capacity sensor surface has been carried out by cyclic dielectric spectroscopy method. The sensor dielectric coating consists of Langmuir-Blodgett monolayers with multiwall modified carbon nanotubes. The nonliner dependence of sensor capacity on cell concentration was registered. At cellular monolayer formation the Helmholtz double layer electrical field shielding effect was shown to arise. It has been established that capacity characteristics of the sensor with adhering monocytes depend on a number and cell continuity as well as ability of cells to generate reactive oxygen species. It has been shown that cell activation by adhesion and fMLP chemotactic peptide leads to increase of capacity of sensor coated by dense monocyte monolayer

Monitoring of Blood Phagocyte Functional State by Cyclic Dielectric Spectroscopy

The study of functional state of blood phagocytes adhering to capacity sensor surface has been carried out by cyclic dielectric spectroscopy method. The sensor dielectric coating consists of Langmuir-Blodgett monolayers with multiwall modified carbon nanotubes. The nonliner dependence of sensor capacity on cell concentration was registered. At cellular monolayer formation the Helmholtz double layer electrical field shielding effect was shown to arise. It has been established that capacity characteristics of the sensor with adhering monocytes depend on a number and cell continuity as well as ability of cells to generate reactive oxygen species. It has been shown that cell activation by adhesion and fMLP chemotactic peptide leads to increase of capacity of sensor coated by dense monocyte monolayer