Click chemistry in ultra-high vacuum - tetrazine coupling with methyl enol ether covalently linked to Si(001)

The additive-free tetrazine/enol ether click reaction was performed in ultra-high vacuum (UHV) with an enol ether group covalently linked to a silicon surface: Dimethyl 1,2,4,5-tetrazine-3,6-dicarboxylate molecules were coupled to the enol ether group of a functionalized cyclooctyne which was adsorbed on the silicon (001) surface via the strained triple bond of cyclooctyne. The reaction was observed at a surface temperature of 380 K by means of X-ray photoelectron spectroscopy (XPS). No indications for tetrazine molecules which bind directly to the Si(001) surface via the nitrogen atoms were detected. A moderate energy barrier was deduced for this click reaction in vacuum by means of density functional theory based calculations, in good agreement with the experimental results. This UHV-compatible click reaction thus opens a new, flexible route for synthesizing covalently bound organic architectures

Report and preliminary results of R/V POSEIDON cruise POS500, LISA, Ligurian Slope AUV mapping, gravity coring and seismic reflection, Catania (Italy) – Malaga (Spain), 25.05.2016 – 09.06.2016

Cruise POS500 “LISA” with R/V Poseidon studied the western Ligurian Margin off Southern France, an area in the northeastern part of the western Mediterranean Sea characterized by its active tectonism and frequent mass wasting. The region near the Var estuary close to the city of Nice is particularly suited for landslide research because it represents a natural laboratority where it is possible to study a series of trigger processes of geological and anthropogenic origin. The aim of this MARUM expedition was to: i. Study fresh water seepage in the marine Nice airport landslide and adjacent stable plateau in 15-50 m water depth using water sampling, CTD and geochemistry; ii. Recover and deploy a number of observatories that monitor, pressure, temperature, tilt and seismicity; iii. Run an AUV micro-bathymetric survey with MARUM AUV SEAL5000 to complement existing multibeam maps; and iv. Acquire additional high-resolution seismic reflection profiles to unravel the complex architecture of the Nice slope and Var delta. In a period of approximately two weeks, we acquired valuable geophysical information that helps to understand the evolution of this portion of the Ligurian Margin and further to support an active Amphibious Drilling proposal submitted to ICDP and IODP. We could also show that heavy spring rainfall plus melt water from the French Maritime Alps supplied sufficient hydraulic forcing to push Var aquifer groundwaters to seep into the marine deposits and water column. Freshening was strongest in the 1979 Nice landslide scar, but was also found at the outer edge of the shelf. Recovery and redeployment of various observatory prototypes worked well, both for the MARUM MeBo seafloor drillstring tolos and independent piezometers. Observatory data have yet to be evaluated. In addition, geochemical analyses of bottom waters and pore waters was deferred to shore-based laboratorios except for salinity estimates using a refractometer. Seismic processing was started onboard, but is largely taking place post-cruise at University Bremen

The Cytotoxic T Lymphocyte Antigen-4+49A/G Single Nucleotide Polymorphism Association With Visceral Leishmaniasis

Background: Several lines of evidence approve that innate and adaptive immunity play key roles in the defense against visceral leishmaniasis (VL). The polymorphism within the cytotoxic T lymphocyte antigen 4 (CTLA-4) gene alters its expression. Objectives: The main aim of this study was to evaluate the polymorphism within the +49 position of the CTLA-4 gene of Iranian patients with VL in comparison with healthy controls. Materials and Methods: In this cross-sectional study, 88 patients with clinical presentations of VL, who were seropositive for Leishmania (group 1), 86 patients without clinical presentations but seropositive (group 2), and 115 healthy controls (group 3) were assessed with respect to the CTLA-4 +49A/G polymorphism, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The anti-Leishmania antibody titration was evaluated using an immunofluorescence method. Results: Our results indicated that both CTLA-4 +49A/G polymorphisms were significantly associated with VL. Conclusions: According to the results, the polymorphisms within the +49 position of CTLA-4 can be associated with VL and may be considered as risk factors for the disease

Bacterial porin disrupts mitochondrial membrane potential and sensitizes host cells to apoptosis

The bacterial PorB porin, an ATP-binding beta-barrel protein of pathogenic Neisseria gonorrhoeae, triggers host cell apoptosis by an unknown mechanism. PorB is targeted to and imported by host cell mitochondria, causing the breakdown of the mitochondrial membrane potential (delta psi m). Here, we show that PorB induces the condensation of the mitochondrial matrix and the loss of cristae structures, sensitizing cells to the induction of apoptosis via signaling pathways activated by BH3-only proteins. PorB is imported into mitochondria through the general translocase TOM but, unexpectedly, is not recognized by the SAM sorting machinery, usually required for the assembly of beta-barrel proteins in the mitochondrial outer membrane. PorB integrates into the mitochondrial inner membrane, leading to the breakdown of delta psi m. The PorB channel is regulated by nucleotides and an isogenic PorB mutant defective in ATP-binding failed to induce delta psi m loss and apoptosis, demonstrating that dissipation of delta psi m is a requirement for cell death caused by neisserial infection