Experiment K-6-01. Distribution and biochemistry of mineral and matrix in the femurs of rats

Previous analyses of the composition of mineral and matrix in the bone of young rats following space flight has revealed deficits in calcium, phosphorus, and osteocalcin, a non-collagenous protein, without an associated decrease in collagen. To characterize the location and nature of this mineralization defect in a weight bearing long bone, the femur, researchers attempted to relate the spatial distribution of mineral in situ in the proximal, central and distal thirds of the femoral diaphysis to the biochemical composition of bone from the same area. Biochemical analyses revealed lower concentrations of calcium, phosphorus and osteocalcin but not collagen only in the central third of the diaphysis of the flight animals (F) compared to synchronous controls (S). Collagen concentration was reduced only in the proximal third of the diaphysis, where all 3 crosslinks, expressed as nM/mol collagen were higher in F than S. A new technique, x ray microtomography, with a resolution of 26 microns, was used to obtain semi-quantitative data on mineral distribution in reconstructed sections of wet whole bone. To improve the resolution of the mineral density distribution, images of the surfaces of cut sections were analyzed by backscattered electrons in a scanning electron microscope (BSE). There was good agreement between the results of the two stereochemical techniques which revealed distinct patterns of mineralization in transverse and longitudinal directions of the diaphysis. The novel methodology developed for this flight experiment shows considerable promise in elucidating the biochemical nature of what appear to be regional alterations in the mineralization of long bones of animals exposed to spaceflight

Lemur Biorhythms and Life History Evolution

Skeletal histology supports the hypothesis that primate life histories are regulated by a neuroendocrine rhythm, the Havers-Halberg Oscillation (HHO). Interestingly, subfossil lemurs are outliers in HHO scaling relationships that have been discovered for haplorhine primates and other mammals. We present new data to determine whether these species represent the general lemur or strepsirrhine condition and to inform models about neuroendocrine-mediated life history evolution. We gathered the largest sample to date of HHO data from histological sections of primate teeth (including the subfossil lemurs) to assess the relationship of these chronobiological measures with life history-related variables including body mass, brain size, age at first female reproduction, and activity level. For anthropoids, these variables show strong correlations with HHO conforming to predictions, though body mass and endocranial volume are strongly correlated with HHO periodicity in this group. However, lemurs (possibly excepting Daubentonia) do not follow this pattern and show markedly less variability in HHO periodicity and lower correlation coefficients and slopes. Moreover, body mass is uncorrelated, and brain size and activity levels are more strongly correlated with HHO periodicity in these animals. We argue that lemurs evolved this pattern due to selection for risk-averse life histories driven by the unpredictability of the environment in Madagascar. These results reinforce the idea that HHO influences life history evolution differently in response to specific ecological selection regimes

On the Unusual Depletions toward Sk 155, or What Are the Small Magellanic Cloud Dust Grains Made of?

The dust in the Small Magellanic Cloud (SMC), an ideal analog of primordial galaxies at high redshifts, differs markedly from that in the Milky Way by exhibiting a steeply rising far-ultraviolet extinction curve, an absence of the 2175 Angstrom extinction feature, and a local minimum at ~12 micron in its infrared emission spectrum, suggesting the lack of ultrasmall carbonaceous grains (i.e. polycyclic aromatic hydrocarbon molecules) which are ubiquitously seen in the Milky Way. While current models for the SMC dust all rely heavily on silicates, recent observations of the SMC sightline toward Sk 155 indicated that Si and Mg are essentially undepleted and the depletions of Fe range from mild to severe, suggesting that metallic grains and/or iron oxides, instead of silicates, may dominate the SMC dust. However, in this Letter we apply the Kramers-Kronig relation to demonstrate that neither metallic grains nor iron oxides are capable of accounting for the observed extinction; silicates remain as an important contributor to the extinction, consistent with current models for the SMC dust.Comment: 12 pages, 3 figures; The Astrophysical Journal Letters, in pres

Simultaneous Ultraviolet and X-ray Observations of the Seyfert Galaxy NGC 4151. II. Physical Conditions in the UV Absorbers

We present a detailed analysis of the intrinsic absorption in the Seyfert 1 galaxy NGC 4151 using UV spectra from the HST/STIS and FUSE, obtained 2002 May as part of a set of contemporaneous observations that included Chandra/HETGS spectra. In our analysis of the Chandra spectra, we determined that the soft X-ray absorber was the source of the saturated UV lines of O VI, C IV, and N V associated with the absorption feature at a radial velocity of ~ -500 km/sec, which we referred to as component D+E. In the present work, we have derived tighter constrains on the the line-of-sight covering factors, densities, and radial distances of the absorbers. We find that the Equivalent Widths (EWs) of the low-ionization lines associated with D+E varied over the period from 1999 July to 2002 May. The drop in the EWs of these lines between 2001 April and 2002 May are suggestive of bulk motion of gas out of our line-of-sight. If these lines from these two epochs arose in the same sub-component, the transverse velocity of the gas is ~ 2100 km/sec. Transverse velocities of this order are consistent with an origin in a rotating disk, at the roughly radial distance we derived for D+E.Comment: 51 pages, including 12 figures. Accepted for publication in ApJ Supplement

Acceleration and Substructure Constraints in a Quasar Outflow

We present observations of probable line-of-sight acceleration of a broad absorption trough of C IV in the quasar SDSS J024221.87+004912.6. We also discuss how the velocity overlap of two other outflowing systems in the same object constrains the properties of the outflows. The Si IV doublet in each system has one unblended transition and one transition which overlaps with absorption from the other system. The residual flux in the overlapping trough is well fit by the product of the residual fluxes in the unblended troughs. For these optically thick systems to yield such a result, at least one of them must consist of individual subunits rather than being a single structure with velocity-dependent coverage of the source. If these subunits are identical, opaque, spherical clouds, we estimate the cloud radius to be r = 3.9 10^15 cm. If they are identical, opaque, linear filaments, we estimate their width to be w = 6.5 10^14 cm. These subunits are observed to cover the Mg II broad emission line region of the quasar, at which distance from the black hole the above filament width is equal to the predicted scale height of the outer atmosphere of a thin accretion disk. Insofar as that scale height is a natural size scale for structures originating in an accretion disk, these observations are evidence that the accretion disk can be a source of quasar absorption systems. Based on data from ESO program 075.B-0190(A).Comment: 14 emulateapj pages, 7 figures, ApJ in pres

Complex X-ray Absorption and the Fe Kalpha Profile in NGC 3516

We present data from simultaneous Chandra, XMM-Newton and BeppoSAX observations of the Seyfert 1 galaxy NGC 3516, taken during 2001 April and Nov. We have investigated the nature of the very flat observed X-ray spectrum. Chandra grating data show the presence of X-ray absorption lines, revealing two distinct components of the absorbing gas, one which is consistent with our previous model of the UV/X-ray absorber while the other, which is outflowing at a velocity of ~1100 km/s has a larger column density and is much more highly ionized. The broad-band spectral characteristics of the X-ray continuum observed with XMM during 2001 April, reveal the presence of a third layer of absorption consisting of a very large column (~2.5 x 10E23 cm^-2) of highly ionized gas with a covering fraction ~50%. This low covering fraction suggests that the absorber lies within a few lt-days of the X-ray source and/or is filamentary in structure. Interestingly, these absorbers are not in thermal equilibrium with one another. The two new components are too highly ionized to be radiatively accelerated, which we suggest is evidence for a hydromagnetic origin for the outflow. Applying our model to the Nov dataset, we can account for the spectral variability primarily by a drop in the ionization states of the absorbers, as expected by the change in the continuum flux. When this complex absorption is accounted for we find the underlying continuum to be typical of Seyfert 1 galaxies. The spectral curvature attributed to the high column absorber, in turn, reduces estimates of the flux and extent of any broad Fe emission line from the accretion disk.Comment: 33 pages, 9 figures, accepted for publication in Ap

STIS Echelle Observations of the Seyfert Galaxy NGC 4151: Physical Conditions in the Ultraviolet Absorbers

We have examined the physical conditions in intrinsic UV-absorbing gas in the Seyfert galaxy NGC 4151, using echelle spectra obtained with the Space Telescope Imaging Spectrograph (STIS). We confirm the presence of the kinematic components detected in earlier GHRS observations as well as a new broad absorption feature at a radial velocity of -1680 km/s. The UV continuum of NGC 4151 decreased by a factor of 4 over the previous two years, and we argue the changes in the column density of the low ionization absorption lines associated with the broad component at -490 km/s reflect the decrease in the ionizing flux. Most of the strong absorption lines (e.g., N V, C IV, Si IV) from this component are saturated, but show substantial residual flux in their cores, indicating that the absorber does not fully cover the source of emission. Our interpretation is that the unocculted light is due to scattering by free electrons from an extended region, which reflects continuum, emission lines, and absorption lines. We have been able to constrain the densities for the kinematic components based on absorption lines from metastable states of C III and Fe II, and/or the ratios of ground and fine structure lines of O I,C II, and Si II. We have generated a set of photoionization models which match the ionic column densities for each component during the present low flux state and those seen in previous high flux states with the GHRS and STIS, confirming that the absorbers are photoionized and respond to the changes in the continuum flux. We have been able to map the relative radial positions of the absorbers, and find that the gas decreases in density with distance. None of the UV absorbers is of sufficiently large column density or high enough ionization state to account for the X-ray absorption.Comment: 46 pages (Latex), 14 figures (postscript), plus a landscape table (Latex), to appear in the Astrophysical Journa

Variable UV Absorption in the Seyfert 1.5 Galaxy NGC 3516: The Case for Associated UV and X-ray Absorption

We present observations of the UV absorption lines in the Seyfert 1 galaxy NGC 3516, obtained at a resolution of $\lambda$/$\Delta\lambda$ $\approx$ 40,000 with the Space Telescope Imaging Spectrograph (STIS) on 2000 October 1. The UV continuum was $\sim$4 times lower than that observed during 1995 with the Goddard High Resolution Spectrograph (GHRS), and the X-ray flux from a contemporaneous {\it Chandra X-ray Observatory (CXO)} observation was a factor of $\sim$8 below that observed with {\it ASCA}. The STIS spectra show kinematic components of absorption in Ly$\alpha$, C IV, and N V at radial velocities of -376, -183, and -36 km s$^{-1}$ (components 1, 2, and 3+4, respectively), which were detected in the earlier GHRS spectra; the last of these is a blend of two GHRS components that have increased greatly in column density. Four additional absorption components have appeared in the STIS spectra at radial velocities of -692, -837, -994, and -1372 km s$^{-1}$ (components 5 through 8); these may also have been present in earlier low-flux states observed by the {\it International Ultraviolet Explorer (IUE)}. Based on photoionization models, we suggest that the components are arranged in increasing radial distance in the order, 3+4, 2, 1, followed by components 5 -- 8. We have achieved an acceptable fit to the X-ray data using the combined X-ray opacity of the UV components 1, 2 and 3+4. By increasing the UV and X-ray fluxes of these models to match the previous high states, we are able to match the GHRS C IV column densities, absence of detectable C IV absorption in components 5 through 8, and the 1994 {\it ASCA} spectrum. We conclude that variability of the UV and X-ray absorption in NGC 3516 is primarily due to changes in the ionizing flux.Comment: 7 figures (note that Fig6 is not referenced in the .Tex file and must be printed separately). There are 6 tables in the .tex file and an additional 8 tables included as separate .ps files. Accepted for Publication in the Astrophysical Journa

The UV Properties of the Narrow Line Quasar I Zwicky 1

I Zw 1 is the prototype narrow line quasar. We report here the results of our study of the UV emission of I Zw 1 using a high S/N (50-120) spectrum obtained with the HST FOS. The following main new results are obtained: 1. The Mg II and Al III doublets are partially/fully resolved. The measured doublet ratios verify theoretical predictions that the lines are thermalized in the BLR. 2. A weak associated UV absorption system is detected in N~V, and possibly also in C IV and Lya, suggesting an outflow with a velocity of 1870 km/s and velocity dispersion <300 km/s. 3. Lines from ions of increasing ionization level show increasing excess blue wing flux, and an increasing line peak velocity shift, reaching a maximum blueshift of about 2000 km/s for He II 1640. This may indicate an out-flowing component in the BLR, where the ionization level increases with velocity, and which is visible only in the approaching direction. The highest velocity part of this outflow may produce the associated UV absorption system. 4. The small C III] 1909 EW, and the small C III] 1909/Lya and C III] 1909/Si III] 1892 flux ratios indicate a typical BLR density of 10^11, i.e. about an order of magnitude larger than implied by C III] 1909 in most quasars. A BLR component of a higher density is implied by the EW and doublet ratio of the Al III 1857 doublet. 5. Prominent Fe II UV 191 emission is seen, together with weaker line emission at 1294 and 1871 A. These three features have been proposed as evidence for significant Lya pumping of the 8-10 eV levels of Fe II. 6. Significant Fe III emission is present. The Fe III UV 34 and UV 48 multiplets are clearly resolved, and Fe III UV 1, UV 47, UV 50, and UV 68 may also be present. (Shortened version)Comment: 28 pages, 1 table and 7 figures included. Uses aas2pp4.sty. Scheduled for the Astrophysical Journal November 10, 1997 issue, Vol. 48

Dynamics of Warm-Absorbing Gas in Seyfert Galaxies: NGC 5548

A hydromagnetic (MHD) wind from a clumpy molecular accretion disk is invoked to explain observations of warm absorbing (WA) gas in UVX from Sy galaxies. This paper focuses on two issues: (1) compatibility of kinematics and dynamics of MHD wind with the observed properties of WAs; and (2) relationship between the UVX absorptions. We provide an in-depth comparison between the MHD model and the Sy 1 galaxy NGC 5548, which at high spectral resolution exhibits a number of discrete UV absorption components. We find that: (1) the total column densities of Ovii, Oviii and H, are reproduced by constraining the UV ion column densities of Civ and Nv in each component to lie within a factor of 2 of their observed values and optimizing over the possible sets of component ionization states and Civ column densities; (2) the WA exists in the outer part of the wind and is not a continuation of the flow in the BLR; and (3) the WA extends in radial and polar directions and is ionization-stratified. X-ray absorption is found to be heavily biased towards smaller r, and UV absorption originates at larger distances from the central continuum source. We show that the discrete absorption components along the line-of-sight are intrinsically clumpy. Density differences between kinematic components result in a range of ionization and recombination timescales. We further test the applicability of the MHD wind to WAs in general, by constructing a quasi-continuous flow model, and extending it to arbitrary aspect angles. We estimate the fraction of Sy 1s having detectable WAs with larger Ovii column density than Oviii, and the range of total H column densities. We also find that the ratio of Ovii to Oviii optical depths can serve as a new diagnostic of AGN aspect angle.Comment: Latex, 8 postscript figures. Astrophysical Journal, 536, June 10, in pres