Diversity of oat varieties in eliciting the early inflammatory events in celiac disease

Purpose Celiac disease (CD) is an autoimmune enteropathy, triggered by dietary gluten. The only treatment is a strict gluten-free diet. Oats are included in the list of gluten-free ingredients by European Regulation, but the safety of oats in CD is still a matter of debate. The present study examined the capability of different oat cultivars of activating the gliadin-induced transglutaminase-2 (TG2)-dependent events in some in vitro models of CD. In addition, we compared this capability with the electrophoresis pattern of peptic\u2013tryptic digests of the proteins of the oat cultivars. Methods K562(S) cells agglutination, transepithelial electrical resistance of T84-cell monolayers, intracellular levels of TG2 and phosphorylated form of protein 42\u201344 in T84 cells were the early gliadin-dependent events studied. Results The results showed that the Nave oat cultivar elicited these events, whereas Irina and Potenza varieties did not. The ability of a cultivar to activate the above-described events was associated with the electrophoretic pattern of oat proteins and their reactivity to anti-gliadin antibodies. Conclusion We found significant differences among oat cultivars in eliciting the TG2-mediated events of CD inflammation. Therefore, the safety of an oat cultivar in CD might be screened in vitro by means of biochemical and biological assays, before starting a clinical trial to definitely assess its safety

Uso de los tra amientos termico e no termico (alta presion hidrostatica) para la inactivación de enzimas bacterianas metaloproteases presentes en la degradación de leche.

El estudio de la aplicación de la tecnología de alta presión en el tratamiento de la leche para la inactivación de las enzimas proteolíticas puede proporcionar un producto con alta calidad y salubridad diferencial, capaz de investir en un aumento del consumo y de valor agregado, con el fin de beneficiarse de la cadena de producción, con impactos económicos y sociales

The Bohm Interpretation of Quantum Cosmology

I make a review on the aplications of the Bohm-De Broglie interpretation of quantum mechanics to quantum cosmology. In the framework of minisuperspaces models, I show how quantum cosmological effects in Bohm's view can avoid the initial singularity, isotropize the Universe, and even be a cause for the present observed acceleration of the Universe. In the general case, we enumerate the possible structures of quantum space and time.Comment: 28 pages, 1 figure, contribution to the James Cushing festschrift to appear in Foundations of Physic

Unveiling the disc structure in ultraluminous X-ray source NGC 55 ULX-1

© 2022 The Author(s). Published by Oxford University Press on behalf of Royal Astronomical Society. This is the accepted manuscript version of an article which has been published in final form athttps://doi.org/10.1093/mnras/stac2453Despite two decades of studies, it is still not clear whether ULX spectral transitions are due to stochastic variability in the wind or variations in the accretion rate or in the source geometry. The compact object is also unknown for most ULXs. In order to place constraints onto such scenarios and on the structure of the accretion disc, we studied the temporal evolution of the spectral components of the variable source NGC 55 ULX-1. Using recent and archival data obtained with the XMM-Newton satellite, we modelled the spectra with two blackbody components which we interpret as thermal emission from the inner accretion flow and the regions around or beyond the spherization radius. The luminosity-temperature (L-T) relation of each spectral component agrees with the L proportional T^4 relationship expected from a thin disc model, which suggests that the accretion rate is close to the Eddington limit. However, there are some small deviations at the highest luminosities, possibly due to an expansion of the disc and a contribution from the wind at higher accretion rates. Assuming that such deviations are due to the crossing of the Eddington or supercritical accretion rate, we estimate a compact object mass of 6-14 Msun, favouring a stellar-mass black hole as the accretor.Peer reviewe

An ultrafast outflow in the black hole candidate MAXI J1810-222?

The transient X-ray source MAXI J1810-222 was discovered in 2018 and has been active ever since. A long combined radio and X-ray monitoring campaign was performed with ATCA and Swift respectively. It has been proposed that MAXI J1810-222 is a relatively distant black hole X-ray binary, albeit showing a very peculiar outburst behaviour. Here, we report on the spectral study of this source making use of a large sample of NICER observations performed between 2019 February and 2020 September. We detected a strong spectral absorption feature at $\sim$1 keV, which we have characterised with a physical photoionisation model. Via a deep scan of the parameters space, we obtained evidence for a spectral-state dependent outflow, with mildly relativistic speeds. In particular, the soft and intermediate states point to a hot plasma outflowing at 0.05-0.15 $c$. This speeds rule-out thermal winds and, hence, they suggest that such outflows could be radiation pressure or (most likely) magnetically-driven winds. Our results are crucial to test current theoretical models of wind formation in X-ray binaries.Comment: 6 pages, 10 figures, accepted for publication in MNRAS Lette

Effects of intravenous furosemide plus small-volume hypertonic saline solutions on markers of heart failure

Aims: We sought to compare the effects of furosemide + hypertonic saline solution (HSS) treatment in patients with acute decompensated heart failure in comparison with furosemide alone and the response in a compensated state after an acute saline load with regard to serum levels of heart failure biomarkers. Methods and results: We enrolled 141 patients with acute decompensated heart failure with reduced ejection fraction admitted to our Internal Medicine ward from March 2017 to November 2019. A total of 73 patients were randomized to treatment with i.v. high-dose furosemide plus HSS, whereas 68 patients were randomized to i.v. high-dose furosemide alone. Patients treated with furosemide plus HSS compared with controls treated with furosemide alone showed a comparable degree of reduction in the serum levels of interleukin (IL)-6, soluble suppression of tumorigenicity 2 (sST2), and N-terminal pro-brain natriuretic peptide (NT-proBNP) in the ‘between-group’ analysis. Nevertheless, patients treated with high-dose furosemide + HSS showed significantly higher absolute delta values of IL-6 (2.3 ± 1.2 vs. 1.7 ± 0.9, P < 0.0005, and 2.0 ± 0.8 vs. 1.85 ± 1.1, P = 0.034), sST2 (41.2 ± 8.6 vs. 27.9 ± 7.6, P < 0.0005, and 37.1 ± 6.6 vs. 28.4 ± 6.7, P < 0.0005), high-sensitivity troponin T (0.03 ± 0.02 vs. 0.02 ± 0.01, P = 0.001, and 0.03 ± 0.02 vs. 0.02 ± 0.01, P = 0.009), NT-proBNP (7237 ± 7931 vs. 3244 ± 4159, P < 0.005, and 5381 ± 4829 vs. 4466 ± 4332, P = 0.004), and galectin-3 (15.7 ± 3.2 ng/mL vs. 11.68 ± 1.9 ng/mL, P < 0.0005, and 16.7 ± 3.9 ng/mL vs. 11.8 ± 2.4 ng/mL, P < 0.0005) than patients treated with furosemide alone. After acute saline load, patients treated with i.v. furosemide + HSS in comparison with subjects treated with furosemide alone showed a significantly lower increase in the serum concentrations of IL-6 (−0.26 ± 0.42 pg/mL vs. −1.43 ± 0.86 pg/mL, P < 0.0005), high-sensitivity troponin T (0 vs. −0.02 ± 0.02 ng/mL, P < 0.0005), sST2 (−8.5 ± 5.9 ng/mL vs. −14.6 ± 6.2 ng/mL, P < 0.0005), galectin-3 (−2.1 ± 1.5 ng/mL vs. −7.1 ± 3.6 ng/mL, P < 0.0005), and NT-proBNP (77 ± 1373 vs. −1706 ± 2259 pg/mL, P < 0.0005). Conclusions: Our findings concerning a comparable degree of reduction in the serum levels of three cardinal biomarkers indicate that a reduction in serum heart failure markers is not linked to the higher degree of congestion relief with a more rapid achievement of a clinical compensation state. This issue may have possible benefits on clinical practice concerning its therapeutic effects over and beyond the simple amelioration of clinical congestion signs and symptoms. Nevertheless, our findings of higher delta values after treatment with i.v. furosemide plus HSS indicate a possible higher efficacy by means of modulation of the stretching and fibrosis mechanisms

Assessment of heart rate variability (HRV) in subjects with type 2 diabetes mellitus with and without diabetic foot: correlations with endothelial dysfunction indices and markers of adipo-inflammatory dysfunction

Background: Some studies have suggested that patients with diabetes and foot complications have worse cardiovascular and cerebrovascular risk profiles, higher degrees of endothelial dysfunction and arterial stiffness and a higher inflammatory background than patients with diabetes without diabetic foot complications. Patients with diabetes mellitus have an alteration in the sympathovagal balance as assessed by means of heart rate variability (HRV) analysis, which is also related to the presence of endothelial dysfunction. Other studies suggest a possible role of inflammation coexisting with the alteration in the sympathovagal balance in favor of the atherosclerotic process in a mixed population of healthy subjects of middle and advanced age. Aims: The aim of this study was to evaluate the degree of alteration of sympathovagal balance, assessed by HRV analysis, in a cohort of patients with diabetes mellitus with diabetic foot and in control subjects without diabetic foot compared with a population of healthy subjects and the possible correlation of HRV parameters with inflammatory markers and endothelial dysfunction indices. Methods: We enrolled all patients with diabetic ulcerative lesions of the lower limb in the Internal Medicine with Stroke Care ward and of the diabetic foot outpatient clinic of P. Giaccone University Hospital of Palermo between September 2019 and July 2020. 4-h ECG Holter was performed. The following time domain HRV measures were analyzed: average heart rate, square root of the mean of successive differences of NN (RMSSD), standard deviation or square root of the variance (SD), and standard deviation of the means of the NN intervals calculated over a five-minute period (SDANN/5 min). The LF/HF ratio was calculated, reactive hyperemia was evaluated by endo-PAT, and serum levels of vaspine and omentin-1 were assessed by blood sample collection. Results: 63 patients with diabetic foot, 30 patients with diabetes and without ulcerative complications and 30 patients without diabetes were enrolled. Patients with diabetic ulcers showed lower mean diastolic blood pressure values than healthy controls, lower MMSE scores corrected for age, lower serum levels of omentin-1, lower RHI values, higher body weight values and comparable body height values, HF% and LF/HF ratio values. We also reported a negative correlation between the RHI value and HRV indices and the expression of increased parasympathetic activity (RMSDD and HF%) in subjects with diabetic foot and a statistically significant positive correlation with the LF/HF ratio and the expression of the sympathovagal balance. Discussion: Patients with diabetic foot show a higher degree of activation of the parasympathetic system, expressed by the increase in HF values, and a lower LF/HF ratio. Our findings may corroborate the issue that a parasympathetic dysfunction may have a possible additive role in the pathogenesis of other vascular complications in subjects with diabetic foot

A case of infective colitis due to Yersinia enterocolitica complicated by microliver abscesses mimicking multiple liver occult metastases: a case report

Background: We report an unusual case of infective colitis by Yersinia enterocolitica complicated by microliver abscesses mimicking multiple liver metastases in a 79 yr old female without any risk factors for bacteriaemia by this pathogen. Case presentation: The patient was admitted to the Internal Medicine with Stroke Care ward of University Policlinico “P. Giaccone” in Palermo because of the appearance of diarrhoea. After the antimicrobial treatment for infective colitis, the clinicians observed a persistently increased white blood cells (WBC) count and multiple hepatic lesions; after having excluded any neoplastic disease and inflammatory bowel disease (IBD), blood cultures positive for Y. enterocolitica allowed to establish the final diagnosis was infective micro liver abscesses consequent to infective colitis due to Y. enterocolitica, which were successfully treated with cefixime and doxycycline. Conclusions: This case report should make clinicians reflect on how complex the differential diagnosis between microliver abscesses and metastasis could be and the possibility of bacteriaemia by Y. enterocolitica even without iron overload conditions