Biological control of Agrobacterium vitis using non-tumorigenic agrobacteria

The potential use of non-tumorigenic agrobacteria for the biological control of grapevine crown gall in Italy was investigated. Four Agrobacterium strains belonging to the species radiobacter and vitis were used to protect the susceptible cv. Malvasia Istriana grafted on the rootstock 420 A. Moreover, the effect of each treatment on grapevine vitality and growth was assessed, including the percentage of marketable vines, as determined by industry standards. Treatments with the antagonists clearly reduced tissue colonization by the pathogen, with a drop of more than 100-fold in pathogen populations in the samples collected at the graft point. Another important effect was the reduction of internal necrosis possibly induced by the high concentration of the nopaline strain CG 49 used in the experiments. According to viticultural and commercial parameters, treatments with the antagonists improved the quality of the vines, with fewer discards and a high percentage of marketable material. Therefore, these antagonists can be considered beneficial for grapevine

Altered gait parameters in distracted walking: a bio-evolutionary and prognostic health perspective on passive listening and active responding during cell phone use

The underpinnings of bipedal gait are reviewed from an evolutionary biology and prognostic health perspective to better understand issues and concerns related to cell phone use during ambulation and under conditions of distraction and interference. We also consider gait-related health issues associated with the fear of or risk of falling and include prognostic dimensions associated with cognitive decline, dementia, and mortality. Data were acquired on 21 healthy young adults without hearing loss, vestibular, balance, otological or neurological dysfunction using a computerized walkway (GAITRite® Walkway System) combined with specialized software algorithms to extract gait parameters. Four experimental conditions and seven temporo-spatial gait parameters were studied: gait velocity, cadence, stride length, ambulatory time, single-support time, double-support time, and step count. Significant main effects were observed for ambulation time, velocity, stride velocity, and double-support time. The greatest impact of distraction and interference occurred during the texting condition, although other significant effects occurred when participants were verbally responding to queries and passively listening to a story. These experimental observations show that relatively simple distraction and interference tasks implemented through the auditory sensory modality can induce significant perturbations in gait while individuals were ambulating and using a cell phone. Herein, emphasis is placed on the use of quantifiable gait parameters in medical, psychological, and audiological examinations to serve as a foundation for identifying and potentially averting gait-related disturbances

A New Measurement of Kaonic Hydrogen X rays

The $\bar{K}N$ system at threshold is a sensitive testing ground for low energy QCD, especially for the explicit chiral symmetry breaking. Therefore, we have measured the $K$-series x rays of kaonic hydrogen atoms at the DA$\Phi$NE electron-positron collider of Laboratori Nazionali di Frascati, and have determined the most precise values of the strong-interaction energy-level shift and width of the $1s$ atomic state. As x-ray detectors, we used large-area silicon drift detectors having excellent energy and timing resolution, which were developed especially for the SIDDHARTA experiment. The shift and width were determined to be $\epsilon_{1s} = -283 \pm 36 \pm 6 {(syst)}$ eV and $\Gamma_{1s} = 541 \pm 89 {(stat)} \pm 22 {(syst)}$ eV, respectively. The new values will provide vital constraints on the theoretical description of the low-energy $\bar{K}N$ interaction.Comment: 5 figures, submitted to Physics Letters

An Optimal Self-Stabilizing Firing Squad

Consider a fully connected network where up to $t$ processes may crash, and all processes start in an arbitrary memory state. The self-stabilizing firing squad problem consists of eventually guaranteeing simultaneous response to an external input. This is modeled by requiring that the non-crashed processes "fire" simultaneously if some correct process received an external "GO" input, and that they only fire as a response to some process receiving such an input. This paper presents FireAlg, the first self-stabilizing firing squad algorithm. The FireAlg algorithm is optimal in two respects: (a) Once the algorithm is in a safe state, it fires in response to a GO input as fast as any other algorithm does, and (b) Starting from an arbitrary state, it converges to a safe state as fast as any other algorithm does.Comment: Shorter version to appear in SSS0

Comprehensive application of a coupled-channel complex scaling method to the KbarN-piY system

We have applied the coupled-channel complex scaling method (ccCSM) to K^{bar}N-\pi Y system. One advantage of ccCSM is that resonant states as well as scattering states can be treated in the same framework. For the interactions in the system, we have constructed a meson-baryon potential-matrix by basing on the chiral SU(3) theory and respecting the K^{bar}N scattering length obtained in the Martin's analysis. For future purpose to apply it more complicated system such as K^{bar}NN, we adopt a local Gaussian form in the r-space. We have investigated both the non-relativistic (NR) and the semi-relativistic (SR) kinematics. In the SR case, two types of the potentials are obtained. To test the constructed potentials, we have calculated scattering amplitudes and searched resonances. One resonance pole, corresponding to \Lambda(1405), is found in isospin I=0 system around (1419, -20) MeV ((1425, -25) or (1419, -13) MeV) on complex-energy plane with the NR (SR) kinematics. Mean distance between meson and baryon in the resonant state is 1.3 - i0.3 fm (1.2 - i0.5 fm) for NR (SR), in which the states are treated as Gamow states. In addition, we have observed a signature of another pole in lower-energy region involving large decay width, although they are unstable against the change of scaling angle \theta. This may correspond to the lower pole of the double-pole of \Lambda(1405) discussed in literature to date.Comment: 51 pages, 17 figures, to appear in Nuclear Physics

Hot-water treatment of dormant grape cuttings: Its effects on Agrobacterium tumefaciens and on grafting and growth of vine

Hot-water treatment (50°C for 20-30 min) was carried out to confirm its efficacy in eradicating Agrobacterium tumefaciens biovar 3 (AT3) in symptomless grape cuttings.After the forcing period, analyses of callus from cuttings of grape cvs Albana, Lambrusco Grasparossa, Rulander and Fortana, and from their graft combinations with the rootstocks 420A, 41B, 5BB and 1103P, revealed the low infection level in the grape material used. Dormant scion and rootstock cuttings treated identically in the U.S. gave similar results. Despite this, it was possible to confirm the efficacy of thermotherapy in eradicating the pathogen.An assessment was also made of the effect of treatment on growth parameters of grafted vines in the greenhouse and after 8 months in a field nursery. The effect of hot-water treatment on the vitality and growth of vines varied with the different scion-rootstock combinations. Treatment did not generally have detrimental effects on vitality; there were some negative effects on graft-take. The number and length of canes, as well as the diameter of the trunks, increased in most instances.The treatments and times usually did not affect bud survival and, in most cases, increased the level of callus formation at the base of cuttings.

Development of liquid helium-3 target for experimental studies of antikaon-nucleon interaction at J-PARC

A liquid helium-3 target system was developed for experimental studies of kaonic atoms and kaonic nuclei at J-PARC. helium-3 gas is liquefied in a heat exchanger cooled below 3.2 K by decompression of liquid helium-4. To maintain a large acceptance of the cylindrical detector system for decay particles of kaonic nuclei, efficient heat transport between the separate target cell and the main unit is realized using circulation of liquid helium-3. To minimize the amount of material, a vacuum vessel containing a carbon fiber reinforced plastic cylinder having an inside diameter of 150 mm and a thickness of 1 mm was produced. A target cell made of pure beryllium and beryllium-aluminum alloy was developed not only to minimize the amount of material but to obtain also high x-ray transmission. During a cooling test, the target cell was kept at 1.3 K at a pressure of 33 mbar. The total estimated heat load to the components including the target cell and heat exchanger cooled by liquid helium-4 decompression, was 0.21 W, and the liquid helium-4 consumption rate was 50 L/day.Comment: 14 pages, 9 figures, submitted to Nuclear Instruments and Methods in Physics Research Section

Novel pathogen-specific primers for the detection of Agrobacterium vitis and Agrobacterium tumefaciens

To detect agrobacteria causing crown gall disease of grapevine novel virulence and oncogene specific primer combinations were tested on Agrobacterium vitis and Agrobacterium tumefaciens strains including most opine types found in grapevines. Reproducible detection of all the tested pathogens in a single reaction was only possible with multiplex PCR using mixtures of virulence-, or oncogene specific primers. A primer combination including pehA, virF and virD2 gene-specific oligonucleotides amplified the corresponding fragments from nearly all strains included and distinguished A. vitis and A. tumefaciens strains carrying octopine or nopaline pTis and A. vitis vitopine strains. A second set of primers designed to amplify the T-DNA auxin genes iaaH and iaaM detected all of the tested pathogens and, as in the case of virF-, and virD2-specific primers, A. vitis vitopine strains formed also a distinct group. These data were further confirmed using opine synthase-, or 6b gene-specific primers that also allowed the identification and distinction of octopine and nopaline as well as vitopine isolates of A. vitis. Thus, a wide range of agrobacteria occurring on grapevine were detected and identified. On the other hand, our results confirm that vitopine-type agrobacteria form a distinct group within the genus Agrobacterium.

First measurement of kaonic helium-3 X-rays

The first observation of the kaonic 3He 3d - 2p transition was made using slow K- mesons stopped in a gaseous 3He target. The kaonic atom X-rays were detected with large-area silicon drift detectors using the timing information of the K+K- pairs of phi-meson decays produced by the DAFNE e+e- collider. The strong interaction shift of the kaonic 3He 2p state was determined to be -2+-2 (stat)+-4 (syst) eV.Comment: Accepted for publication in Phys. Lett.

Investigation of high frequency coupling between probe tips and wafer surface

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