Diffusion-limited deposition of dipolar particles

Deposits of dipolar particles are investigated by means of extensive Monte Carlo simulations. We found that the effect of the interactions is described by an initial, non-universal, scaling regime characterized by orientationally ordered deposits. In the dipolar regime, the order and geometry of the clusters depend on the strength of the interactions and the magnetic properties are tunable by controlling the growth conditions. At later stages, the growth is dominated by thermal effects and the diffusion-limited universal regime obtains, at finite temperatures. At low temperatures the crossover size increases exponentially as T decreases and at T=0 only the dipolar regime is observed.Comment: 5 pages, 4 figure

The influence of oxygen concentration during embryo culture on obstetric and neonatal outcomes: a secondary analysis of a randomized controlled trial.

STUDY DESIGN, SIZE, DURATION: A secondary analysis of a previous randomized controlled trial assessing clinical pregnancy outcomes was carried out. This analysis included 1125 consecutive oocyte donation cycles utilizing ICSI or IVF and Day 3 embryo transfers between November 2009 and April 2012. The whole cohort of donated oocytes from patients who agreed to participate in the study were randomly allocated (1:1 ratio) to a reduced O2 tension group (6% O2) or an air-exposed group (20% O2) based on a computergenerated randomization list. Fresh and vitrified oocytes were used for oocyte donation. Only those pregnancies with a live birth at or beyond 24 weeks of gestation were included. PARTICIPANTS/MATERIALS, SETTING, METHODS: Day 3 embryos were cultured in an atmosphere of 5.5% CO2, 6% O2, 88.5% N2 versus a dual gas system in air. MAIN RESULTS AND THE ROLE OF CHANCE: From the eligible 1125 cycles, 564 were allocated to the 6% O2 group and 561 cycles to the 20% O2 group. However, 50 and 62 cycles did not reach embryo transfer in the 6% and 20% O2 groups, respectively. No differences were found between 6% O2 and atmospheric O2 tension in the number of livebirths per embryo transfer (mean § SD, 0.5 § 0.7 versus 0.5 § 0.7), pregnancy complications or neonatal outcomes. Both groups (6% and atmospheric O2) had similar single and twin delivery rates (40.8% versus 38.1% and 10.7% versus 12.3%, respectively). Preterm delivery rates and very preterm delivery rates (10.80% versus 13.24% and 1.25% versus 2.94%, respectively), birthweight (3229 § 561 g versus 3154 § 731 g), low birthweight (2.92% versus 2.45%), birth height (50.18 § 2.41 cm versus 49.7 § 3.59 cm), head circumference (34.16 § 1.87 cm versus 33.09 § 1.85 cm) and 1 min Apgar scores (8.96 § 0.87 versus 8.89 § 0.96) were also similar between 6% and atmospheric O2 groups, respectively. LIMITATIONS, REASONS FOR CAUTION: The number of liveborns finally analyzed is still small and not all obstetric and neonatal variables could be evaluated. Furthermore, a small proportion of the obstetric and neonatal data was obtained through a questionnaire VC The Author(s) 2020. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please email: [email protected] Human Reproduction, Vol.0, No.0, pp. 1-14, 2020 doi:10.1093/humrep/deaa152 One reason for the lack of effect of oxygen concentration on pregnancy outcome could be the absence of trophectoderm cells at cleavage stage, which may make Day 3 embryos less susceptible to hypoxic conditions. WIDER IMPLICATIONS OF THE FINDINGS: Nowadays many IVF laboratories use a more physiological oxygen concentration for embryo culture. However, the benefits of using low oxygen concentration on both laboratory and clinical outcomes during embryo culture are still under debate. Furthermore, long-term studies investigating the effect of using atmospheric O2 are also needed. Gathering these type of clinical data is indeed, quite relevant from the safety perspective. The present data show that, at least in egg donation cycles undergoing Day 3 embryo transfers, culturing embryos under atmospheric oxygen concentration seems not to affect perinatal outcome

Critical wetting of a class of nonequilibrium interfaces: A mean-field picture

A self-consistent mean-field method is used to study critical wetting transitions under nonequilibrium conditions by analyzing Kardar-Parisi-Zhang (KPZ) interfaces in the presence of a bounding substrate. In the case of positive KPZ nonlinearity a single (Gaussian) regime is found. On the contrary, interfaces corresponding to negative nonlinearities lead to three different regimes of critical behavior for the surface order-parameter: (i) a trivial Gaussian regime, (ii) a weak-fluctuation regime with a trivially located critical point and nontrivial exponents, and (iii) a highly non-trivial strong-fluctuation regime, for which we provide a full solution by finding the zeros of parabolic-cylinder functions. These analytical results are also verified by solving numerically the self-consistent equation in each case. Analogies with and differences from equilibrium critical wetting as well as nonequilibrium complete wetting are also discussed.Comment: 11 pages, 2 figure

Finite size effects in nonequilibrium wetting

Models with a nonequilibrium wetting transition display a transition also in finite systems. This is different from nonequilibrium phase transitions into an absorbing state, where the stationary state is the absorbing one for any value of the control parameter in a finite system. In this paper, we study what kind of transition takes place in finite systems of nonequilibrium wetting models. By solving exactly a microscopic model with three and four sites and performing numerical simulations we show that the phase transition taking place in a finite system is characterized by the average interface height performing a random walk at criticality and does not discriminate between the bounded-KPZ classes and the bounded-EW class. We also study the finite size scaling of the bKPZ universality classes, showing that it presents peculiar features in comparison with other universality classes of nonequilibrium phase transitions.Comment: 14 pages, 6figures, major change

Variation in flexural, morphological, and biochemical leaf properties of eelgrass (Zostera marina) along the European Atlantic climate regions

Seagrasses need to withstand hydrodynamic forces; therefore, mechanical properties such as flexibility or breaking resistance are beneficial for survival. The co-variation of leaf breaking properties with biochemical traits in seagrasses has been documented, but it is unknown if the same patterns apply to leaf flexural properties. To interpret changes in the ecological function of seagrass ecosystems based on acclimation responses to environmental changes, it is necessary to understand the factors that affect flexural leaf properties. Here, morphological and flexural leaf properties of the perennial type of Zostera marina across different environmental conditions along European Atlantic climate regions are presented together with C:N ratio and neutral detergent fibre content as descriptors of biochemical leaf composition. Eelgrass leaves from cold regions were similar to threefold more elastic and similar to tenfold more flexible, were also narrower (1.7-fold), and contained similar to 1.9-fold higher fibre content than from plants growing in warmer regions. Eelgrass also showed acclimation to local conditions such as seasonality, water depth, and hydrodynamic exposure. Leaves collected from exposed or shallower locations or during winter were more flexible, suggesting an avoidance strategy to hydrodynamic forcing, which is generally higher under those conditions. Flexural rigidity was almost equally controlled by bending modulus (35%) and leaf thickness (37%), indicating functional differences compared to leaf breaking described in the literature. Overall, the findings indicate that Zostera marina has a high flexural plasticity and high acclimation capacity to some climate change effects such as sea level rise and increase in storm frequency and intensity.German Science FoundationGerman Research Foundation (DFG) [PA 2547/1-1]Royal Swedish Academy of Sciences (KVA travel grant)FCT-Foundation for Science and TechnologyPortuguese Foundation for Science and Technology [UID/Multi/04326/2019, SFRH/BPD/119344/2016

Engineering Transcriptional Regulator Effector Specificity using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor

The pursuit of circuits and metabolic pathways of increasing complexity and robustness in synthetic biology will require engineering new regulatory tools. Feedback control based on relevant molecules, including toxic intermediates and environmental signals, would enable genetic circuits to react appropriately to changing conditions. In this work, variants of qacR, a tetR family repressor, were generated by computational protein design and screened in a cell-free transcription–translation (TX-TL) system for responsiveness to a new targeted effector. The modified repressors target vanillin, a growth-inhibiting small molecule found in lignocellulosic hydrolysates and other industrial processes. Promising candidates from the in vitro screen were further characterized in vitro and in vivo in a gene circuit. The screen yielded two qacR mutants that respond to vanillin both in vitro and in vivo. While the mutants exhibit some toxicity to cells, presumably due to off-target effects, they are prime starting points for directed evolution toward vanillin sensors with the specifications required for use in a dynamic control loop. We believe this process, a combination of the generation of variants coupled with in vitro screening, can serve as a framework for designing new sensors for other target compounds

Nonequilibrium wetting transitions with short range forces

We analyze within mean-field theory as well as numerically a KPZ equation that describes nonequilibrium wetting. Both complete and critical wettitng transitions were found and characterized in detail. For one-dimensional substrates the critical wetting temperature is depressed by fluctuations. In addition, we have investigated a region in the space of parameters (temperature and chemical potential) where the wet and nonwet phases coexist. Finite-size scaling analysis of the interfacial detaching times indicates that the finite coexistence region survives in the thermodynamic limit. Within this region we have observed (stable or very long-lived) structures related to spatio-temporal intermittency in other systems. In the interfacial representation these structures exhibit perfect triangular (pyramidal) patterns in one (two dimensions), that are characterized by their slope and size distribution.Comment: 11 pages, 5 figures. To appear in Physical Review

Critical wetting of a class of nonequilibrium interfaces: A computer simulation study

Critical wetting transitions under nonequilibrium conditions are studied numerically and analytically by means of an interface-displacement model defined by a Kardar-Parisi-Zhang equation, plus some extra terms representing a limiting, short-ranged attractive wall. Its critical behavior is characterized in detail by providing a set of exponents for both the average height and the surface order-parameter in one dimension. The emerging picture is qualitatively and quantitatively different from recently reported mean-field predictions for the same problem. Evidence is shown that the presence of the attractive wall induces an anomalous scaling of the interface local slopes.Comment: 7 pages, 8 figure

Viability of competing field theories for the driven lattice gas

It has recently been suggested that the driven lattice gas should be described by a novel field theory in the limit of infinite drive. We review the original and the new field theory, invoking several well-documented key features of the microscopics. Since the new field theory fails to reproduce these characteristics, we argue that it cannot serve as a viable description of the driven lattice gas. Recent results, for the critical exponents associated with this theory, are re-analyzed and shown to be incorrect.Comment: 4 pages, revtex, no figure