Genome-wide association analysis of milk yield traits in Nordic Red Cattle using imputed whole genome sequence variants

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Nebular-Phase Spectra of Nearby Type Ia Supernovae

We present late-time spectra of eight Type Ia supernovae (SNe Ia) obtained at $>200$ days after peak brightness using the Gemini South and Keck telescopes. All of the SNe Ia in our sample were nearby, well separated from their host galaxy's light, and have early-time photometry and spectroscopy from the Las Cumbres Observatory (LCO). Parameters are derived from the light curves and spectra such as peak brightness, decline rate, photospheric velocity, and the widths and velocities of the forbidden nebular emission lines. We discuss the physical interpretations of these parameters for the individual SNe Ia and the sample in general, including comparisons to well-observed SNe Ia from the literature. There are possible correlations between early-time and late-time spectral features that may indicate an asymmetric explosion, so we discuss our sample of SNe within the context of models for an offset ignition and/or white dwarf collisions. A subset of our late-time spectra are uncontaminated by host emission, and we statistically evaluate our nondetections of H$\alpha$ emission to limit the amount of hydrogen in these systems. Finally, we consider the late-time evolution of the iron emission lines, finding that not all of our SNe follow the established trend of a redward migration at $>200$ days after maximum brightness.Comment: 20 pages, 8 figures, 9 tables; accepted to MNRA

Identifications of novel mechanisms in breast cancer cells involving duct-like multicellular spheroid formation after exposure to the Random Positioning Machine

Many cell types form three-dimensional aggregates (MCS; multicellular spheroids), when they are cultured under microgravity. MCS often resemble the organ, from which the cells have been derived. In this study we investigated human MCF-7 breast cancer cells after a 2 h-, 4 h-, 16 h-, 24 h-and 5d-exposure to a Random Positioning Machine (RPM) simulating microgravity. At 24 h few small compact MCS were detectable, whereas after 5d many MCS were floating in the supernatant above the cells, remaining adherently (AD). The MCS resembled the ducts formed in vivo by human epithelial breast cells. In order to clarify the underlying mechanisms, we harvested MCS and AD cells separately from each RPM-culture and measured the expression of 29 selected genes with a known involvement in MCS formation. qPCR analyses indicated that cytoskeletal genes were unaltered in short-term samples. IL8, VEGFA, and FLT1 were upregulated in 2 h/4 h AD-cultures. The ACTB, TUBB, EZR, RDX, FN1, VEGFA, FLK1 Casp9, Casp3, PRKCA mRNAs were downregulated in 5d-MCS-samples. ESR1 was upregulated in AD, and PGR1 in both phenotypes after 5d. A pathway analysis revealed that the corresponding gene products are involved in organization and regulation of the cell shape, in cell tip formation and membrane to membrane docking

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity

The literature suggests morphological alterations and molecular biological changes within the cellular milieu of human cells, exposed to microgravity (mu g), as many cell types assemble to multicellular spheroids (MCS). In this study we investigated juvenile normal human dermal fibroblasts (NHDF) grown in simulated mu g (s-mu g) on a random positioning machine (RPM), aiming to study changes in cell morphology, cytoskeleton, extracellular matrix (ECM), focal adhesion and growth factors. On the RPM, NHDF formed an adherent monolayer and compact MCS. For the two cell populations we found a differential regulation of fibronectin, laminin, collagen-IV, aggrecan, osteopontin, TIMP-1, integrin-beta(1), caveolin-1, E-cadherin, talin-1, vimentin, alpha-SM actin, TGF-beta(1), IL-8, MCP-1, MMP-1, and MMP-14 both on the transcriptional and/or translational level. Immunofluorescence staining revealed only slight structural changes in cytoskeletal components. Flow cytometry showed various membrane-bound proteins with considerable variations. In silico analyses of the regulated proteins revealed an interaction network, contributing to MCS growth via signals mediated by integrin-beta(1), E-cadherin, caveolin-1 and talin-1. In conclusion, s-mu g-conditions induced changes in the cytoskeleton, ECM, focal adhesion and growth behavior of NHDF and we identified for the first time factors involved in fibroblast 3D-assembly. This new knowledge might be of importance in tissue engineering, wound healing and cancer metastasis

Estimation of Genotype × Environment interaction in soybean (Glycine max (L.) Merrill) crosses for grain yield and its component traits under different sowing dates

The study was conducted at AICRP on Soybean, Zonal Agricultural Research Station, UAS, GKVK, Bengaluru, to detect and quantify the stable performing genotypes by temporal environment interaction. Fifty-eight genetically stable soybean lines selected from F5 population of four crosses were evaluated with two checks in α-lattice design with two replications, across three dates of sowing namely, 20thJanuary, 20th February and 20th March during, 2022. Pooled ANOVA revealed a significant difference for number of pods plant-1 and grain yield plant-1 attributable to genotypes × dates of sowing. The AMMI ANOVA indicated a significant difference attributable to GEI for number of pods plant-1, and grain yield-1. Based on the lower estimates of AMMI stability value and stability index for grain yield plant-1 lines 3, 23, 8, 5 and 14 in cross JS24-34 × RVS2001-18, 10, 3, 7 and 2 lines in cross JS20-34 × RVS2001-18, lines 9, 10, 6 and 8 in cross DSb-31 × MACS1460 and lines 12, 5, 1, 8 and 4 in cross DSb-21 × EC457254 were regarded as widely adaptable and stable genotypes

Increased yield of smear positive pulmonary TB cases by screening patients with >2 weeks cough, compared to >3 weeks and adequacy of 2 sputum smear examinations for diagnosis

Background RNTCP recommends examining three sputum smears for AFB from Chest Symptomatics (CSs) with cough of >3 weeks for diagnosis of Pulmonary TB (PTB). A previous multi-centric study from Tuberculosis Research centre (TRC) has shown that the yield of sputum positive cases can be increased if duration of cough for screening was reduced to >2 weeks. Other studies have shown that two smear examinations are adequate for diagnosis of smear positive PTB . To validate the above findings, a cross sectional multi-centric study was repeated in different settings in five geographical areas in India. Methods Three primary and secondary level health facilities with high out-patient attendance were selected from two Tuberculosis Units (TU) in each of the 15 selected districts to screen about 10,000 new adult outpatients from each state. For patients who did not volunteer history of cough, symptoms were elicited using a structured simple questionnaire. All the CSs were referred for sputum examination. Results A total of 96,787 out-patients were registered. Among them 69,209 (72%) were new adult out-patients. Using >2 weeks of cough instead of > 3 weeks as the criterion for screening, there was an overall increase of 58% in CS and 23% increase in the detection of smear-positive cases. Among 211 patients, 210 were positive at least by one smear from the initial two specimens. Increase in the work-load if 2 smears were done for patients with cough of >2 weeks cough were 2 specimens (i.e.13 to 15) per day for an adult OPD of 150. Conclusion The yield of sputum positive PTB cases can be improved by screening patients with >2 weeks cough and two specimens are adequate for diagnosis

Local Genealogies in a Linear Mixed Model for Genome-Wide Association Mapping in Complex Pedigreed Populations

INTRODUCTION: The state-of-the-art for dealing with multiple levels of relationship among the samples in genome-wide association studies (GWAS) is unified mixed model analysis (MMA). This approach is very flexible, can be applied to both family-based and population-based samples, and can be extended to incorporate other effects in a straightforward and rigorous fashion. Here, we present a complementary approach, called 'GENMIX (genealogy based mixed model)' which combines advantages from two powerful GWAS methods: genealogy-based haplotype grouping and MMA. SUBJECTS AND METHODS: We validated GENMIX using genotyping data of Danish Jersey cattle and simulated phenotype and compared to the MMA. We simulated scenarios for three levels of heritability (0.21, 0.34, and 0.64), seven levels of MAF (0.05, 0.10, 0.15, 0.20, 0.25, 0.35, and 0.45) and five levels of QTL effect (0.1, 0.2, 0.5, 0.7 and 1.0 in phenotypic standard deviation unit). Each of these 105 possible combinations (3 h(2) x 7 MAF x 5 effects) of scenarios was replicated 25 times. RESULTS: GENMIX provides a better ranking of markers close to the causative locus' location. GENMIX outperformed MMA when the QTL effect was small and the MAF at the QTL was low. In scenarios where MAF was high or the QTL affecting the trait had a large effect both GENMIX and MMA performed similarly. CONCLUSION: In discovery studies, where high-ranking markers are identified and later examined in validation studies, we therefore expect GENMIX to enrich candidates brought to follow-up studies with true positives over false positives more than the MMA would

Nanoscale Hybrid Electrolytes with Viscosity Controlled Using Ionic Stimulus for Electrochemical Energy Conversion and Storage

As renewable energy is rapidly integrated into the grid, the challenge has become storing intermittent renewable electricity. Technologies including flow batteries and CO 2 conversion to dense energy carriers are promising storage options for renewable electricity. To achieve this technological advancement, the development of next generation electrolyte materials that can increase the energy density of flow batteries and combine CO 2 capture and conversion is desired. Liquid-like nanoparticle organic hybrid materials (NOHMs) composed of an inorganic core with a tethered polymeric canopy (e.g., polyetheramine (HPE)) have a capability to bind chemical species of interest including CO 2 and redox-active species. In this study, the unique response of NOHM-I-HPE-based electrolytes to salt addition was investigated, including the effects on solution viscosity and structural configurations of the polymeric canopy, impacting transport behaviors. The addition of 0.1 M NaCl drastically lowered the viscosity of NOHM-based electrolytes by up to 90%, reduced the hydrodynamic diameter of NOHM-I-HPE, and increased its self-diffusion coefficient, while the ionic strength did not alter the behaviors of untethered HPE. This study is the first to fundamentally discern the changes in polymer configurations of NOHMs induced by salt addition and provides a comprehensive understanding of the effect of ionic stimulus on their bulk transport properties and local dynamics. These insights could be ultimately employed to tailor transport properties for a range of electrochemical applications