Incidence of some pathogenic organisms in cultured shrimp (penaeus monodon) collected from Chilaw

Samples offarm shrimp and pond water were collectedfrom Chi/aw. Totalbacterial counts of coliforms and Escherichia coli are reportedfor these samples.Pathogens Vibrio parahaemolyticus, and Salmonella along with qualitativemicroflora are also reported.The bacterial counts of water rangedfrom 5 x 102/gto 8.8 x 103/g whereasthat of shrimp rangedfrom 2.0 x IO"/g to 9.0 x lO'/g. E. coli. ranged from 3 toII/g for prawns 3/I00ml. to 49/I00ml. for pond water. Two shrimp sampleswere positive for v. parahaemolyticus bacterial counts, coliforms or E. colicounts did not show any correlation with water salinity, which rangedfrom 14-18ppt,Gram positive bacteria were predominant in shrimp and were representedby Micrococci (41.8%). Corynebacterium (19.3%) and Bacillus (14.2%).Common gram-negatives were Vibrios (19.2%) and Pseudomonas (5%)

Child Labour Utilization in the Tourism Industry: A Case Study from Ampara District in Sri Lanka

The tourism industry is considered as one of the world's fastest growing economic activities and the largest employer of manpower which generates millions of employment opportunities. Since tourism is a labour intensive industry there is an escalating demand for labour in different sectors of tourism. This demand becomes a critical issue due to reasons such as the scarcity of the trained and skilled human resource, lower salaries and wages and unwillingness among many people to pursue tourism-related employment. As a remedy to this critical issue, child labour has been used frequently. They have been employed in both formal and informal sectors of the tourism industry.  This situation is more common in the Sri Lanka tourism industry, especially in remote tourism regions.  The main objective of this research was to study the nature of child labour in the tourism industry of the Ampara district of Eastern Tourism region of Sri Lanka. After a preliminary survey, Ulla, Panama North and Panama West GNDs of the Ampara district of Eastern tourism region were selected as the study area. Applying judgment and purposive sampling techniques, 56 child workers and 19 accommodation establishments were selected as the sample. Both quantitative and qualitative methods such as questionnaire surveys, observations, interviews, group discussions and case studies were employed to collect data. The data was analyzed applying both qualitative and quantitative analytical methods. The overall findings of the study are discussed under several subheadings including the socioeconomic and family background of child workers, the existing nature and types of child labour, reasons for selecting of employment linked to tourism, places of work, salaries and wages, the nature of expenditure and problems faced by child workers. The overall findings of this study revealed that child workers have been employed in formal and informal sectors of the tourism industry. All the child labours are in the age range between 15 to 17 years. The most common form of utilizing child labour in the study area is as sellers. Economic burdens at home are the major reasons which draw the children to these jobs. Engaging in different activities, they are able to earn an income ranging from Rs. 3,000 to 15,000 and they have spent this income on different purposes. Since there are many problems related to child labour utilization active policies and plans are timely and significant to alleviate these problems.  KEYWORDS: Tourism Industry, Child Labour, Labour utilizatio

A key role for peroxynitrite-mediated inhibition of cardiac ERG (Kv11.1) K+ channels in carbon monoxide–induced proarrhythmic early afterdepolarizations

Exposure to carbon monoxide (CO) causes early afterdepolarization arrhythmias. Previous studies in rats indicated arrhythmias arose due to augmentation of the late Na+ current. The purpose of the present study was to examine the basis of CO-induced arrhythmias in guinea pig myocytes in which action potentials more closely resemble those of human myocytes. Whole-cell current- and voltage-clamp recordings were made from isolated guinea pig myocytes and also from HEK293 cells expressing wild-type or a C723S mutant form of Kv11.1 (ERG). We also monitored formation of peroxynitrite (ONOO-) in HEK293 cells fluorimetrically. CO, applied as the CO releasing molecule, CORM-2, prolonged action potentials and induced early after-depolarizations (EADs) in guinea pig myocytes. In HEK293 cells CO inhibited wild-type but not C723S mutant Kv11.1 K+ currents. Inhibition was prevented by an antioxidant, mitochondrial inhibitors or inhibition of nitric oxide formation. CO also raised ONOO- levels, an effect reversed by the ONOO- scavenger, FeTPPS which also prevented CO inhibition of Kv11.1 currents, and abolished the effects of CO on Kv11.1 tail currents and action potentials in guinea pig myocytes. Our data suggest that CO induces arrhythmias in guinea pig cardiac myocytes via ONOO--mediated inhibition of Kv11.1 K+ channel

The potential of harnessing real-time occupancy data for improving energy performance of activity-based workplaces

Currently, the available studies on the prediction of building energy performance and real occupancy data are typically characterized by aggregated and averaged occupancy patterns or large thermal zones of reference. Despite the increasing diffusion of smart energy management systems and the growing availability of longitudinal data regarding occupancy, these two domains rarely inform each other. This research aims at understanding the potential of employing real-time occupancy data to identify better cooling strategies for activity-based-working (ABW)-supportive offices and reduce the overall energy consumption. It presents a case study comparing the energy performance of the office when different resolutions of occupancy and thermal zoning are applied, ranging from the standard energy certification approach to real-time occupancy patterns. For the first time, one year of real-time occupancy data at the desk resolution, captured through computer logs and Bluetooth devices, is used to investigate this issue. Results show that the actual cooling demand is 9% lower than predicted, unveiling the energy-saving potential to be achieved from HVAC systems for non-assigned seating environments. This research demonstrates that harnessing real-time occupancy data for demand-supply cooling management at a fine-grid resolution is an efficient strategy to reduce cooling consumption and increase workers’ comfort. It also emphasizes the need for more data and monitoring campaigns for the definition of more accurate and robust energy management strategies

Barrier dysfunction or drainage reduction: differentiating causes of CSF protein increase

BACKGROUND Cerebrospinal fluid (CSF) protein analysis is an important element in the diagnostic chain for various central nervous system (CNS) pathologies. Among multiple existing approaches to interpreting measured protein levels, the Reiber diagram is particularly robust with respect to physiologic inter-individual variability, as it uses multiple subject-specific anchoring values. Beyond reliable identification of abnormal protein levels, the Reiber diagram has the potential to elucidate their pathophysiologic origin. In particular, both reduction of CSF drainage from the cranio-spinal space as well as blood-CNS barrier dysfunction have been suggested ρas possible causes of increased concentration of blood-derived proteins. However, there is disagreement on which of the two is the true cause. METHODS We designed two computational models to investigate the mechanisms governing protein distribution in the spinal CSF. With a one-dimensional model, we evaluated the distribution of albumin and immunoglobulin G (IgG), accounting for protein transport rates across blood-CNS barriers, CSF dynamics (including both dispersion induced by CSF pulsations and advection by mean CSF flow) and CSF drainage. Dispersion coefficients were determined a priori by computing the axisymmetric three-dimensional CSF dynamics and solute transport in a representative segment of the spinal canal. RESULTS Our models reproduce the empirically determined hyperbolic relation between albumin and IgG quotients. They indicate that variation in CSF drainage would yield a linear rather than the expected hyperbolic profile. In contrast, modelled barrier dysfunction reproduces the experimentally observed relation. CONCLUSIONS High levels of albumin identified in the Reiber diagram are more likely to originate from a barrier dysfunction than from a reduction in CSF drainage. Our in silico experiments further support the hypothesis of decreasing spinal CSF drainage in rostro-caudal direction and emphasize the physiological importance of pulsation-driven dispersion for the transport of large molecules in the CSF

Hypoxic gene expression in chronic hepatitis B virus infected patients is not observed in state-of-the-art in vitro and mouse infection models

Hepatitis B virus (HBV) is the leading cause of hepatocellular carcinoma (HCC) worldwide. The prolyl hydroxylase domain (PHD)-hypoxia inducible factor (HIF) pathway is a key mammalian oxygen sensing pathway and is frequently perturbed by pathological states including infection and inflammation. We discovered a significant upregulation of hypoxia regulated gene transcripts in patients with chronic hepatitis B (CHB) in the absence of liver cirrhosis. We used state-of-the-art in vitro and in vivo HBV infection models to evaluate a role for HBV infection and the viral regulatory protein HBx to drive HIF-signalling. HBx had no significant impact on HIF expression or associated transcriptional activity under normoxic or hypoxic conditions. Furthermore, we found no evidence of hypoxia gene expression in HBV de novo infection, HBV infected human liver chimeric mice or transgenic mice with integrated HBV genome. Collectively, our data show clear evidence of hypoxia gene induction in CHB that is not recapitulated in existing models for acute HBV infection, suggesting a role for inflammatory mediators in promoting hypoxia gene expression

Multiple mechanisms mediating carbon monoxide inhibition of the voltage-gated K+ channel Kv1.5

The voltage-gated K+ channel plays key roles in the vasculature and in atrial excitability, and contributes to apoptosis in various tissues. In this study, we have explored its regulation by carbon monoxide (CO), a product of the cytoprotective heme oxygenase enzymes, and a recognized toxin. CO inhibited recombinant Kv1.5 expressed in HEK293 cells in a concentration-dependent manner which involved multiple signalling pathways. CO inhibition was partially reversed by superoxide dismutase mimetics, and by suppression of mitochondrial reactive oxygen species. CO also elevated intracellular nitric oxide (NO) levels. Prevention of NO formation also partially reversed CO inhibition of Kv1.5, as did inhibition of soluble guanylyl cyclase. CO also elevated intracellular peroxynitrite levels, and a peroxynitrite scavenger markedly attenuated the ability of CO to inhibit Kv1.5. CO caused nitrosylation of Kv1.5, an effect which was also observed in C331A and C346A mutant forms of the channel, which had previously been suggested as nitrosylation sites within Kv1.5. Augmentation of Kv1.5 via exposure to hydrogen peroxide was fully reversed by CO. Native Kv1.5 recorded in HL-1 murine atrial cells was also inhibited by CO. Action potentials recorded in HL-1 cells were increased in amplitude and duration by CO, an effect mimicked and occluded by pharmacological inhibition of Kv1.5. Our data indicate that Kv1.5 is a target for modulation by CO via multiple mechanisms. This regulation has important implications for diverse cellular functions, including excitability, contractility and apoptosis

Omeprazole Inhibits Proliferation and Modulates Autophagy in Pancreatic Cancer Cells

BACKGROUND: Omeprazole has recently been described as a modulator of tumour chemoresistance, although its underlying molecular mechanisms remain controversial. Since pancreatic tumours are highly chemoresistant, a logical step would be to investigate the pharmacodynamic, morphological and biochemical effects of omeprazole on pancreatic cancer cell lines. METHODOLOGY/PRINCIPAL FINDINGS: Dose-effect curves of omeprazole, pantoprazole, gemcitabine, 5-fluorouracil and the combinations of omeprazole and 5-fluorouracil or gemcitabine were generated for the pancreatic cancer cell lines MiaPaCa-2, ASPC-1, Colo357, PancTu-1, Panc1 and Panc89. They revealed that omeprazole inhibited proliferation at probably non-toxic concentrations and reversed the hormesis phenomena of 5-fluorouracil. Electron microscopy showed that omeprazole led to accumulation of phagophores and early autophagosomes in ASPC-1 and MiaPaCa-2 cells. Signal changes indicating inhibited proliferation and programmed cell death were found by proton NMR spectroscopy of both cell lines when treated with omeprazole which was identified intracellularly. Omeprazole modulates the lysosomal transport pathway as shown by Western blot analysis of the expression of LAMP-1, Cathepsin-D and β-COP in lysosome- and Golgi complex containing cell fractions. Acridine orange staining revealed that the pump function of the vATPase was not specifically inhibited by omeprazole. Gene expression of the autophagy-related LC3 gene as well as of Bad, Mdr-1, Atg12 and the vATPase was analysed after treatment of cells with 5-fluorouracil and omeprazole and confirmed the above mentioned results. CONCLUSIONS: We hypothesise that omeprazole interacts with the regulatory functions of the vATPase without inhibiting its pump function. A modulation of the lysosomal transport pathway and autophagy is caused in pancreatic cancer cells leading to programmed cell death. This may circumvent common resistance mechanisms of pancreatic cancer. Since omeprazole use has already been established in clinical practice these results could lead to new clinical applications

Self-assembled amyloid fibrils with controllable conformational heterogeneity

Amyloid fibrils are a hallmark of neurodegenerative diseases and exhibit a conformational diversity that governs their pathological functions. Despite recent findings concerning the pathological role of their conformational diversity, the way in which the heterogeneous conformations of amyloid fibrils can be formed has remained elusive. Here, we show that microwave-assisted chemistry affects the self-assembly process of amyloid fibril formation, which results in their conformational heterogeneity. In particular, microwave-assisted chemistry allows for delicate control of the thermodynamics of the self-assembly process, which enabled us to tune the molecular structure of ??-lactoglobulin amyloid fibrils. The heterogeneous conformations of amyloid fibrils, which can be tuned with microwave-assisted chemistry, are attributed to the microwave-driven thermal energy affecting the electrostatic interaction during the self-assembly process. Our study demonstrates how microwave-assisted chemistry can be used to gain insight into the origin of conformational heterogeneity of amyloid fibrils as well as the design principles showing how the molecular structures of amyloid fibrils can be controlledopen0