In vitro assessment of cytotoxicity of giomer on human gingival fibroblasts

Root coverage on restored root surfaces has been considered as a challenging issue. The evaluation of cytotoxic effects of restorative materials is a fundamental requirement for sustaining the cell attachment and the clinical success of root coverage. The aim of the present study was to compare the human gingival fibroblast cytotoxicity of the recently introduced giomer composite (GC) with resin ionomer (RI) restorative material. Discs (6x2 mm) of GC and RI restorative materials were prepared using sterile Teflon mold. Extracts from the materials were incubated to cell culture medium for 24, 48 and 72 h. Human gingival fibroblasts (HGF) were exposed to the extracts of the materials while the un-incubated media served as the control group. The cytotoxicity of the materials were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In order to compare the mean values of the measured parameters a Kruskal-Walis test was carried out. MTT assay indicated that human gingival fibroblasts proliferated well in the presence of GC extract. The proliferation rate was higher in cells incubated with GC compared to RI extracts but the differences were not statistically significant (p= 0.09). This in vitro study indicated that GC is a non-toxic material for HGF. However, further studies are needed to assess the other biologic and clinical behavior of this material prior to it being considered as a potentially suitable restorative material to restore the carious root lesions candidated to root coverage procedures

In vitro assessment of cytotoxicity of giomer on human gingival fibroblasts

Root coverage on restored root surfaces has been considered as a challenging issue. The evaluation of cytotoxic effects of restorative materials is a fundamental requirement for sustaining the cellattachment and the clinical success of root coverage. The aim of the present study was to compare the human gingival fibroblast cytotoxicity of the recently introduced giomer composite (GC) with resinionomer (RI) restorative material. Discs (6×2 mm) of GC and RI restorative materials were prepared using sterile Teflon mold. Extracts from the materials were incubated to cell culture medium for 24, 48and 72 h. Human gingival fibroblasts (HGF) were exposed to the extracts of the materials while the unincubated media served as the control group. The cytotoxicity of the materials were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In order to compare the mean values of the measured parameters a Kruskal-Walis test was carried out. MTT assay indicated that human gingival fibroblasts proliferated well in the presence of GC extract. The proliferation rate washigher in cells incubated with GC compared to RI extracts but the differences were not statistically significant (p= 0.09). This in vitro study indicated that GC is a non-toxic material for HGF. However, further studies are needed to assess the other biologic and clinical behavior of this material prior to it being considered as a potentially suitable restorative material to restore the carious root lesions candidated to root coverage procedures

The role of rock joint frictional strength in the containment of fracture propagation

The fracturing phenomenon within the reservoir environment is a complex process that is controlled by several factors and may occur either naturally or by artificial drivers. Even when deliberately induced, the fracturing behaviour is greatly influenced by the subsurface architecture and existing features. The presence of discontinuities such as joints, artificial and naturally occurring faults and interfaces between rock layers and microfractures plays an important role in the fracturing process and has been known to significantly alter the course of fracture growth. In this paper, an important property (joint friction) that governs the shear behaviour of discontinuities is considered. The applied numerical procedure entails the implementation of the discrete element method to enable a more dynamic monitoring of the fracturing process, where the joint frictional property is considered in isolation. Whereas fracture propagation is constrained by joints of low frictional resistance, in non-frictional joints, the unrestricted sliding of the joint plane increases the tendency for reinitiation and proliferation of fractures at other locations. The ability of a frictional joint to suppress fracture growth decreases as the frictional resistance increases; however, this phenomenon exacerbates the influence of other factors including in situ stresses and overburden conditions. The effect of the joint frictional property is not limited to the strength of rock formations; it also impacts on fracturing processes, which could be particularly evident in jointed rock masses or formations with prominent faults and/or discontinuities

Numerical modeling of hydraulic fracture propagation, closure and reopening using XFEM with application to in-situ stress estimation

In this paper, a fully coupled model is developed for numerical modeling of hydraulic fracturing in partially saturated weak porous formations using the extended finite element method, which provides an effective means to simulate the coupled hydro-mechanical processes occurring during hydraulic fracturing. The developed model is for short fractures where plane strain assumptions are valid. The propagation of the hydraulic fracture is governed by the cohesive crack model, which accounts for crack closure and reopening. The developed model allows for fluid flow within the open part of the crack and crack face contact resulting from fracture closure. To prevent the unphysical crack face interpenetration during the closing mode, the crack face contact or self-contact condition is enforced using the penalty method. Along the open part of the crack, the leakage flux through the crack faces is obtained directly as a part of the solution without introducing any simplifying assumption. If the crack undergoes the closing mode, zero leakage flux condition is imposed along the contact zone. An application of the developed model is shown in numerical modeling of pump-in/shut-in test. It is illustrated that the developed model is able to capture the salient features bottomhole pressure/time records exhibit and can extract the confining stress perpendicular to the direction of the hydraulic fracture propagation from the fracture closure pressure

Numerical modeling of hydraulic fracture propagation, closure and reopening using XFEM with application to in-situ stress estimation

In this paper, a fully coupled model is developed for numerical modeling of hydraulic fracturing in partially saturated weak porous formations using the extended finite element method, which provides an effective means to simulate the coupled hydro-mechanical processes occurring during hydraulic fracturing. The developed model is for short fractures where plane strain assumptions are valid. The propagation of the hydraulic fracture is governed by the cohesive crack model, which accounts for crack closure and reopening. The developed model allows for fluid flow within the open part of the crack and crack face contact resulting from fracture closure. To prevent the unphysical crack face interpenetration during the closing mode, the crack face contact or self-contact condition is enforced using the penalty method. Along the open part of the crack, the leakage flux through the crack faces is obtained directly as a part of the solution without introducing any simplifying assumption. If the crack undergoes the closing mode, zero leakage flux condition is imposed along the contact zone. An application of the developed model is shown in numerical modeling of pump-in/shut-in test. It is illustrated that the developed model is able to capture the salient features bottomhole pressure/time records exhibit and can extract the confining stress perpendicular to the direction of the hydraulic fracture propagation from the fracture closure pressure

Numerical modeling of two-phase fluid flow in deformable fractured porous media using the extended finite element method and an equivalent continuum model

In the present paper, a numerical model is developed based on a combination of the extended finite element method and an equivalent continuum model to simulate the two-phase fluid flow through fractured porous media containing fractures with multiple length scales. The governing equations involve the linear momentum balance equation and the flow continuity equation for each fluid phase. The extended finite element method allows for an explicit and accurate representation of cracks by enriching the standard finite element approximation of the field variables with appropriate enrichment functions, and captures the mass transfer between the fracture and the matrix. Due to the high computational cost of X-FEM, this technique is only used to model large fractures. The pre-existing short fractures, which are distributed randomly in the porous medium, contribute to the increase of the effective permeability tensor and are modeled with an equivalent continuum model. Finally, the robustness of the proposed computational model is demonstrated through several numerical examples, and the effects of crack orientation, capillary pressure function, solid skeleton deformation, and existence of short cracks on the pattern of fluid flow are investigated. It is shown that the developed model provides a correct prediction of flow pattern for different crack configurations

Numerical modeling of two-phase fluid flow in deformable fractured porous media using the extended finite element method and an equivalent continuum model

In the present paper, a numerical model is developed based on a combination of the extended finite element method and an equivalent continuum model to simulate the two-phase fluid flow through fractured porous media containing fractures with multiple length scales. The governing equations involve the linear momentum balance equation and the flow continuity equation for each fluid phase. The extended finite element method allows for an explicit and accurate representation of cracks by enriching the standard finite element approximation of the field variables with appropriate enrichment functions, and captures the mass transfer between the fracture and the matrix. Due to the high computational cost of X-FEM, this technique is only used to model large fractures. The pre-existing short fractures, which are distributed randomly in the porous medium, contribute to the increase of the effective permeability tensor and are modeled with an equivalent continuum model. Finally, the robustness of the proposed computational model is demonstrated through several numerical examples, and the effects of crack orientation, capillary pressure function, solid skeleton deformation, and existence of short cracks on the pattern of fluid flow are investigated. It is shown that the developed model provides a correct prediction of flow pattern for different crack configurations

Effect of 2-Phenylethanol as Antifungal Agent and Common Antifungals (Amphotericin B, Fluconazole, and Itraconazole) on Candida Species Isolated from Chronic and Recurrent Cases of Candidal Vulvovaginitis

The antifungal effects of 2-phenylethanol are clearly visible through its intervention in Candida morphogenesis. Chronic and recurrent vulvovaginitis, however, does not respond to this standard experimental therapy; therefore, the study presented in this article investigated the effect of common antifungal drugs (amphotericin B AMB, fluconazole FLU, and itraconazole ITC), in combination with 2-phenylethanol, on the Candida species isolated from cases of chronic and recurrent vulvovaginitis, thereby allowing the recommendation of a more appropriate treatment option. Forty isolates from patients with chronic and recurrent vaginal candidiasis were investigated in this experimental study. The specimens were examined by direct microscopy, culturing, and PCR to identify the species. The antifungal effects of 2-phenylethanol and conventional drugs, both alone and in combination, were determined in duplicate. Finally, the findings were analyzed. In this study, 40 strains of Candida species were identified, whose agents were Candida albicans (95%) and Candida africana (5%). After 48 h, the minimum inhibitory concentration (MIC) range of the 2-phenylethanol was 800-3,200 μg/mL. Also, in the final study on the MIC levels of common antifungal drugs, AMB (0.42 μg/mL) had the lowest MIC, FLU (40.51 μg/mL) had the highest MIC, and the combination of ITC and 2-phenylethanol had the lowest fractional inhibitory concentration index (FICI) of any of the combinations (FICI range, 0.26-1.03). Combining FLU and ITC with 2-phenylethanol can effectively increase their antifungal effect. © 2018, Mary Ann Liebert, Inc. 2018