413 research outputs found
Range-only benthic Rover localization off the central California coast
Nowadays, the use of autonomous vehicles for
ocean research has increased, since these vehicles have a better
cost/performance ratio than crewed vessels or oceanographic
ships. For example, autonomous surface vehicles can be used to
localize underwater targets. This paper describes a mission to find
a crawling robot - Benthic Rover - on the abyssal plain in the north
eastern Pacific, using single-beacon localization from onboard a
Wave Glider autonomous surface vehicle. While the Wave Glider
is moving around the surface in the target zone, it takes ranges
between the target and itself using acoustic modems. With these
ranges it can compute the target location, as a Long Baseline
(LBL) system. The benefit of this approach is the reduction of cost
and complexity relative to deployment of a traditional shipboard
LBL system. Additionally, this is a mobile system, and can cover
long distances, and can geolocate multiple targets over a large
area.Postprint (author's final draft
Range-only underwater target localization : error characterization
Locating a target from range measurements
using only one mobile transducer has been increased
over the last years. This method allows us to reduce the
high costs of deployment and maintenance of
traditional fixed systems on the seafloor such as Long
Baseline. The range-only single-beacon is one of the
new architectures developed using the new capabilities
of modern acoustic underwater modems, which can be
time synchronization, time stamp, and range
measurements.
This document presents a method to estimate the
sources of error in this type of architecture so as to
obtain a mathematical model which allows us to
develop simulations and study the best localization
algorithms. Different simulations and real field tests
have been carried out in order to verify a good
performance of the model proposed.Postprint (published version
Acoustic TAG tracking: First experiments
Nowadays, the use of autonomous vehicles for
ocean research has increased, since these vehicles have a better
cost/performance ratio than crewed vessels or oceanographic
ships. For example, autonomous surface vehicles can be used to
localize underwater targets. Whereas different research works are
focused in target tracking using acoustic modems (or USBL), in
this paper a new method called Area-Only target tracking is
presented, which uses the signal generated by acoustic TAGs. This
document, the first tests are presented and their results discussed,
which were conducted in the Monterey Bay.Peer ReviewedPostprint (published version
Prokaryotic responses to a warm temperature anomaly in northeast subarctic Pacific waters
Recent studies on marine heat waves describe water temperature anomalies causing changes in food web structure, bloom dynamics, biodiversity loss, and increased plant and animal mortality. However, little information is available on how water temperature anomalies impact prokaryotes (bacteria and archaea) inhabiting ocean waters. This is a nontrivial omission given their integral roles in driving major biogeochemical fluxes that influence ocean productivity and the climate system. Here we present a time-resolved study on the impact of a large-scale warm water surface anomaly in the northeast subarctic Pacific Ocean, colloquially known as the Blob, on prokaryotic community compositions. Multivariate statistical analyses identified significant depth- and season-dependent trends that were accentuated during the Blob. Moreover, network and indicator analyses identified shifts in specific prokaryotic assemblages from typically particle-associated before the Blob to taxa considered free-living and chemoautotrophic during the Blob, with potential implications for primary production and organic carbon conversion and export. Traving et al. use small subunit ribosomal RNA gene sequencing to examine spatial and temporal trends in bacterial and archaeal community structure during a large marine warm water surface anomaly, the Blob. Their findings suggest that community structure shifted during the Blob, with taxa considered free-living and chemoautotrophic prevailing under these unusual conditions
Fatal anaphylactoid reaction following ioversol administration
We report a fatal intravenous ioversol administration in a 60-year old male patient. Although the introduction of new low-osmolar non-ionogenic contrast media with a more favourable efficacy-toxicity balance has diminished the side-effects significantly, everyone involved in radiodiagnostic procedures should be aware of the potential life-threatening effects. Especially patients with risk factors for side-effects should be monitored carefully
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Can an Off-Nominal Landing by an MMRTG-Powered Spacecraft Induce a Special Region on Mars When No Ice Is Present?
This work aims at addressing whether a catastrophic failure of an entry, descent, and landing event of a Multimission Radioisotope Thermoelectric Generator-based lander could embed the heat sources into the martian subsurface and create a local environment that (1) would temporarily satisfy the conditions for a martian Special Region and (2) could establish a transport mechanism through which introduced terrestrial organisms could be mobilized to naturally occurring Special Regions elsewhere on Mars. Two models were run, a primary model by researchers at the Lawrence Berkeley National Laboratory and a secondary model by researchers at the Jet Propulsion Laboratory, both of which were based on selected starting conditions for various surface composition cases that establish the worst-case scenario, including geological data collected by the Mars Science Laboratory at Gale Crater. The summary outputs of both modeling efforts showed similar results: that the introduction of the modeled heat source could temporarily create the conditions established for a Special Region, but that there would be no transport mechanism by which an introduced terrestrial microbe, even if it was active during the temporarily induced Special Region conditions, could be transported to a naturally occurring Special Region of Mars
HCV IRES manipulates the ribosome to promote the switch from translation initiation to elongation.
The internal ribosome entry site (IRES) of the hepatitis C virus (HCV) drives noncanonical initiation of protein synthesis necessary for viral replication. Functional studies of the HCV IRES have focused on 80S ribosome formation but have not explored its role after the 80S ribosome is poised at the start codon. Here, we report that mutations of an IRES domain that docks in the 40S subunit's decoding groove cause only a local perturbation in IRES structure and result in conformational changes in the IRES-rabbit 40S subunit complex. Functionally, the mutations decrease IRES activity by inhibiting the first ribosomal translocation event, and modeling results suggest that this effect occurs through an interaction with a single ribosomal protein. The ability of the HCV IRES to manipulate the ribosome provides insight into how the ribosome's structure and function can be altered by bound RNAs, including those derived from cellular invaders
Analysis of natural variants of the hepatitis C virus internal ribosome entry site reveals that primary sequence plays a key role in cap-independent translation
The HCV internal ribosome entry site (IRES) spans a region of ∼340 nt that encompasses most of the 5′ untranslated region (5′UTR) of the viral mRNA and the first 24–40 nt of the core-coding region. To investigate the implication of altering the primary sequence of the 5′UTR on IRES activity, naturally occurring variants of the 5′UTR were isolated from clinical samples and analyzed. The impact of the identified mutations on translation was evaluated in the context of RLuc/FLuc bicistronic RNAs. Results show that depending on their location within the RNA structure, these naturally occurring mutations cause a range of effects on IRES activity. However, mutations within subdomain IIId hinder HCV IRES-mediated translation. In an attempt to explain these data, the dynamic behavior of the subdomain IIId was analyzed by means of molecular dynamics (MD) simulations. Despite the loss of function, MD simulations predicted that mutant G266A/G268U possesses a structure similar to the wt-RNA. This prediction was validated by analyzing the secondary structure of the isolated IIId RNAs by circular dichroism spectroscopy in the presence or absence of Mg2+ ions. These data strongly suggest that the primary sequence of subdomain IIId plays a key role in HCV IRES-mediated translation
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