Estimation of badger abundance using faecal DNA typing

1.Wildlife management and conservation programmes often require accurate information on population density, but this can be difficult to obtain, particularly when the species in question is nocturnal or cryptic. Badger populations in Britain are of intense management interest because they are a wildlife reservoir host of bovine tuberculosis (TB). Attempts to manage this infection in badgers, whether by population control or vaccination, require reliable methods of estimating population size. In addition, such estimates are also required to support research into badger ecology and TB epidemiology. Currently, the most accurate estimates of local badger population size are obtained from labour-intensive and time-consuming mark–recapture studies. 2. In recent years, DNA has been successfully extracted from the faeces of certain mammals, and used to generate a genetic profile of the defecating individual. Here we report on an application of this technology to estimate badger abundance.3.Faecal samples were collected on 10 consecutive days from every freshly deposited dropping at latrine sites close to occupied setts in three badger social groups. Badger DNA was extracted from 89% of samples, and 20 different individuals were reliably identified. The genotypes derived from the faecal samples were compared with those obtained from blood or samples from badgers live trapped at the same setts.4.The faecal genotypes from badgers with known trap histories revealed that latrines were used equally by males and females, and by badgers ranging in age from cubs(< 1 year old) to 9 years old. Individual badgers used the latrines on between one and six different nights. Rarefaction analysis produced abundance estimates that closely matched those obtained from live trapping. 5.Synthesis and applications. Systematic sampling and genetic typing of fresh faeces from badger latrines can provide data that can be used to estimate abundance accurately.This approach requires considerably less human resources than repeated live trapping and mark–recapture. The technique may be valuable for future badger research and management in relation to bovine TB, where accurate estimates of abundance at a local scale are required

Exoplanets imaging with a Phase-Induced Amplitude Apodization Coronagraph - I. Principle

Using 2 aspheric mirrors, it is possible to apodize a telescope beam without losing light or angular resolution: the output beam is produced by ``remapping'' the entrance beam to produce the desired light intensity distribution in a new pupil. We present the Phase-Induced Amplitude Apodization Coronagraph (PIAAC) concept, which uses this technique, and we show that it allows efficient direct imaging of extrasolar terrestrial planets with a small-size telescope in space. The suitability of the PIAAC for exoplanet imaging is due to a unique combination of achromaticity, small inner working angle (about 1.5 $\lambda/d$), high throughput, high angular resolution and large field of view. 3D geometrical raytracing is used to investigate the off-axis aberrations of PIAAC configurations, and show that a field of view of more than 100 $\lambda/d$ in radius is available thanks to the correcting optics of the PIAAC. Angular diameter of the star and tip-tilt errors can be compensated for by slightly increasing the size of the occulting mask in the focal plane, with minimal impact on the system performance. Earth-size planets at 10 pc can be detected in less than 30s with a 4m telescope. Wavefront quality requirements are similar to classical techniques.Comment: 35 pages, 16 figures, Accepted for publication in Ap

Status of undergraduate community-based and public-health physiotherapy education in South Africa

Curricula of health education institutions therefore need to be periodically revised to be aligned with its context. This study explored the status physiotherapy curricula in South Africa (SA) as point of departure for benchmarking by individual institutions. A document analysis was done of the university physiotherapy departments (N=8) in South Africa. Institutional ethical clearance and permission from the heads of departments were obtained. Content analysis was used to analyse the South African Qualifications Authority exit-level outcomes and the university study guides for community placements. Most universities employed a form of service-learning, with interventions in a range of settings. Five themes emerged: practice of evidence-based physiotherapy, rendering physiotherapy services, acting professionally, communication, and collaboration. The country’s priority conditions were addressed. Teaching-earning strategies included group activities (class or education sessions), community projects, home visits and portfolios of evidence. Personal and small-group reflections were prominent. The undergraduate community physiotherapy curricula in South Africa address the health profile of the population and priorities in the health system to different degrees. The variation between universities should be interpreted with caution as the study guides only gave a limited snapshot into each institution’s curriculum. However, findings suggest that each physiotherapy university department may have gaps in preparing physiotherapy undergraduate students for the needs of the South African population and expectations of the Government. Possible ways to share teaching-learning resources are recommended.Department of HE and Training approved lis

Do Mitigated Wetlands Support Similar Small Mammal Communities as Natural Wetlands?

Wetlands provide many ecosystem services and play an important ecological role in wildlife communities. Although wetland mitigation is a standard tool to combat losses to natural wetlands, it is essential to understand if mitigated wetlands are truly replacing natural wetlands in their full capacity. Because one important role of wetlands is to provide habitat for wildlife communities, it is important to determine if these created or restored wetlands can foster a wildlife community that is similar to natural wetlands. One understudied taxa in the realm of wetland mitigation research is small mammals. Our objectives are to examine community composition, occupancy, abundance, species diversity, species richness, and species evenness of small mammals at mitigated and natural wetlands to determine if there exists a difference between the two types of wetlands. To conduct this research, we are using Sherman traps for a capture-mark-recapture study on small mammals at mitigated and natural wetlands that are paired by similarities in ecoregion, elevation, geology, and wetland classification. In 2020, ten wetland sites were sampled with a total of 3,875 trap nights and 249 captures. Preliminary data analyses show Peromyscus spp. to be more abundant in natural wetlands than mitigated wetlands, and species richness between the two wetland types not to be statistically different. Results will determine if mitigated wetlands are successful in terms of providing habitat for small mammal communities, and in turn will contribute to whether current wetland mitigation is truly fulfilling its intended purpose. These findings could inform future management decisions

Notes on Recent Cases

Notes on recent cases by Albert T. Frantz, Henry Hasley, Edward P. McGuire, William Coyne, John P. Berscheid, Ivan J. LeBlanc, E. F. McClarnon, Seymour Weisberger, and Thomas J. Griffin

Demographic characteristics of an avian predator, Louisiana Waterthrush (Parkesia motacilla), in response to its aquatic prey in a Central Appalachian USA watershed impacted by shale gas development

We related Louisiana Waterthrush (Parkesia motacilla) demographic response and nest sur- vival to benthic macroinvertebrate aquatic prey and to shale gas development parameters using models that accounted for both spatial and non-spatial sources of variability in a Central Appala- chian USA watershed. In 2013, aquatic prey density and pollution intolerant genera (i.e., pollu- tion tolerance value \u3c4) decreased statistically with increased waterthrush territory length but not in 2014 when territory densities were lower. In general, most demographic responses to aquatic prey were variable and negatively related to aquatic prey in 2013 but positively related in 2014. Competing aquatic prey covariate models to explain nest survival were not statistically significant but differed annually and in general reversed from negative to positive influence on daily survival rate. Potential hydraulic fracturing runoff decreased nest survival both years and was statistically significant in 2014. The EPA Rapid Bioassessment protocol (EPA) and Habitat Suitability Index (HSI) designed for assessing suitability requirements for waterthrush were posi- tively linked to aquatic prey where higher scores increased aquatic prey metrics, but EPA was more strongly linked than HSI and varied annually. While potential hydraulic fracturing runoff in 2013 may have increased Ephemeroptera, Plecoptera, and Trichoptera (EPT) richness, in 2014 shale gas territory disturbance decreased EPT richness. In 2014, intolerant genera decreased at the territory and nest level with increased shale gas disturbance suggesting the potential for localized negative effects on waterthrush. Loss of food resources does not seem directly or solely responsible for demographic declines where waterthrush likely were able to meet their foraging needs. However collective evidence suggests there may be a shale gas dis- turbance threshold at which waterthrush respond negatively to aquatic prey community changes. Density-dependent regulation of their ability to adapt to environmental change through acquisition of additional resources may also alter demographic response

Ancient pigs reveal a near-complete genomic turnover following their introduction to Europe

Archaeological evidence indicates that pig domestication had begun by ∼10,500 y before the present (BP) in the Near East, and mitochondrial DNA (mtDNA) suggests that pigs arrived in Europe alongside farmers ∼8,500 y BP. A few thousand years after the introduction of Near Eastern pigs into Europe, however, their characteristic mtDNA signature disappeared and was replaced by haplotypes associated with European wild boars. This turnover could be accounted for by substantial gene flow from local European wild boars, although it is also possible that European wild boars were domesticated independently without any genetic contribution from the Near East. To test these hypotheses, we obtained mtDNA sequences from 2,099 modern and ancient pig samples and 63 nuclear ancient genomes from Near Eastern and European pigs. Our analyses revealed that European domestic pigs dating from 7,100 to 6,000 y BP possessed both Near Eastern and European nuclear ancestry, while later pigs possessed no more than 4% Near Eastern ancestry, indicating that gene flow from European wild boars resulted in a near-complete disappearance of Near East ancestry. In addition, we demonstrate that a variant at a locus encoding black coat color likely originated in the Near East and persisted in European pigs. Altogether, our results indicate that while pigs were not independently domesticated in Europe, the vast majority of human-mediated selection over the past 5,000 y focused on the genomic fraction derived from the European wild boars, and not on the fraction that was selected by early Neolithic farmers over the first 2,500 y of the domestication process