Quantum engineering of atomic phase-shifts in optical clocks

Quantum engineering of time-separated Raman laser pulses in three-level systems is presented to produce an ultra-narrow optical transition in bosonic alkali-earth clocks free from light shifts and with a significantly reduced sensitivity to laser parameter fluctuations. Based on a quantum artificial complex-wave-function analytical model, and supported by a full density matrix simulation including a possible residual effect of spontaneous emission from the intermediate state, atomic phase-shifts associated to Ramsey and Hyper-Ramsey two-photon spectroscopy in optical clocks are derived. Various common-mode Raman frequency detunings are found where the frequency shifts from off-resonant states are canceled, while strongly reducing their uncertainties at the 10$^{-18}$ level of accuracy.Comment: accepted for publication in PR

Quality evaluation of fresh and bloomed filled chocolates in House of Quality

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Evaluating the effects of generalisation approaches and DEM resolution on the extraction of terrain indices in KwaZulu Natal, South Africa

Digital elevation model (DEM) data are elemental in deriving primary topographic attributes which serve as input variables to a variety of hydrologic and geomorphologic studies. There is however still varied consensus on the effect of DEM source and resolution on the application of these topographic attributes to landscape characterisation. While elevation data for South Africa are available from several major sources and resolutions: Shuttle Radar Topographic Mission (SRTM), Earth ENV and Stellenbosch University DEM (SUDEM). Limited research has been conducted in a local context comparing the extraction of terrain attributes to high resolution Digital Terrain Data (DTM) such as LiDAR (Light Detection and Ranging) that are becoming increasing available. However, the utility of LiDAR to topographic analyses presents its own challenges in terms of operational-relevant resolution, processing demands and limited spatial coverage. There is a need to quantify the impact that generalisation approaches have on simplifying detailed DEMs and to compare the accuracy and reliability of results between high resolution and coarse resolution data on the extraction of localized topographic variables. In this regional study, we analyse the accuracy on selected local terrain attributes: elevation, slope and topographic wetness index derived from DEMs from varying sources, at different spatial resolutions and using three generalisation algorithms, namely: mean cell aggregation, nearest neighbour and hydrological corrected topo-to-raster. We show that topographic variable extraction is highly dependent on DEM source and generalisation approach and while higher resolution DEMs may represent the “true“ surface more accurately, they do not necessarily offer the best results for all extracted variables. Our results highlight the caveats of selecting DEMs not “fit-for-purpose” for topographic analysis and offer a simple yet effective solution for reconciling the selection of DEMs based on neighbourhood size resolution prior to terrain analyses and topographic feature characterization

Total evaporation estimates from a Renosterveld and dryland wheat/fallow surface at the Voëlvlei Nature Reserve (South Africa)

Accurate quantification of the water balance, in particular evapotranspiration, is fundamental in managing water resources, especially in semi-arid areas. The objective of this study was to compare evaporation from endemic vegetation – Renosterveld – and a dryland wheat/fallow cropping system. The study was carried out in the mid-reaches of the Berg River catchment (South Africa), characterised by dryland salinity. Measurements of total evaporation from these 2 land uses were carried out with large aperture scintillometers during window periods from 2005 to 2007. Total evaporation was measured to be higher in Renosterveld than in wheat during the rainy winter season. In the dry summer season, total evaporation from Renosterveld was limited by soil water supply, and vegetation was under water stress. Spatial variability of total evaporation from both wheat/fallow land and Renosterveld was estimated using the Surface Energy Balance Algorithm for Land (SEBAL) model for 3 climatically different years. The scintillometer measurements were used to determine basal crop coefficients for long-term (20 years) simulations with the HYDRUS-1D model to assess temporal variability in total evaporation. Long-term simulations indicated that well-established, deep-rooted Renosterveld uses 39% more water than the shallowrooted wheat/fallow system. A change in land use from Renosterveld to dryland annual crops could therefore affect the soil water balance, cause shallow saline groundwater tables and degradation of soil and water resources.Keywords: evapotranspiration; large aperture scintillometer; Renosterveld; soil water balance; Surface Energy Balance Algorithm for Land (SEBAL); wheat/fallow syste

A multiSite gateway™ vector set for the functional analysis of genes in the model Saccharomyces cerevisiae

<p>Abstract</p> <p>Background</p> <p>Recombinatorial cloning using the Gateway^TM technology has been the method of choice for high-throughput omics projects, resulting in the availability of entire ORFeomes in Gateway^TM compatible vectors. The MultiSite Gateway^TM system allows combining multiple genetic fragments such as promoter, ORF and epitope tag in one single reaction. To date, this technology has not been accessible in the yeast <it>Saccharomyces cerevisiae</it>, one of the most widely used experimental systems in molecular biology, due to the lack of appropriate destination vectors.</p> <p>Results</p> <p>Here, we present a set of three-fragment MultiSite Gateway^TM destination vectors that have been developed for gene expression in <it>S. cerevisiae</it> and that allow the assembly of any promoter, open reading frame, epitope tag arrangement in combination with any of four auxotrophic markers and three distinct replication mechanisms. As an example of its applicability, we used yeast three-hybrid to provide evidence for the assembly of a ternary complex of plant proteins involved in jasmonate signalling and consisting of the JAZ, NINJA and TOPLESS proteins.</p> <p>Conclusion</p> <p>Our vectors make MultiSite Gateway^TM cloning accessible in <it>S. cerevisiae</it> and implement a fast and versatile cloning method for the high-throughput functional analysis of (heterologous) proteins in one of the most widely used model organisms for molecular biology research.</p

De Noordzee: een waardevol archief onder water. Meer dan 100 jaar onderzoek van strandvondsten en vondsten uit zee in België: een overzicht

De Noordzee kan beschouwd worden als een waardevol en speciaal archief, met heel wat interessante informatie over het verleden. De zone beneden de laagwaterlijn behoort tot het domein van de subtidale archeologie of de archeologie van het subgetijdengebied van de Noordzee. De zone tussen de hoog- en de laagwaterlijn behoort tot de intertidale archeologie of de archeologie van het intergetijdengebied van de Noordzee. In het eerste deel van deze studie wordt kort de geschiedenis van het onderzoek in deze beide zones geschetst. Daarna wordt in een tweede deel een chronologisch overzicht gegeven van de resultaten van het onderzoek en dit vanaf het ontstaan van de archeologie als wetenschappelijke discipline. In dit tweede deel wordt ook een klein aantal tot nu toe ongepubliceerde vondsten opgenomen van buiten het Belgische deel van de Noordzee. De reden hiervoor is zowel pragmatisch als inhoudelijk. Enerzijds worden deze vondsten geregistreerd samen met de andere vondsten, ze bevinden zich immers samen in de bestudeerde collecties. Anderzijds dragen ze ook inhoudelijk bij tot een beter inzicht in de genese van het hele zuidelijke Noordzeegebied, waarvan de zone onder Belgisch toezicht deel uitmaakt. Verder dienen in dit tweede deel ook een aantal vraagstellingen en onderzoeksstrategieën als basis voor de globale discussie in het derde deel van deze bijdrage. De bijdrage wordt ten slotte afgesloten met een zo volledig mogelijke bibliografie over het onderzoek in het Belgische deel van de Noordzee inclusief de stranden