Hematology as Related to Diving Characteristics of Elaphe obsoleta, Nerodia erythrogaster, Nerodia Fasciata and Nerodia rhombifera

The diving capabilities of Nerodia erythrogaster flavigaster and Nerodia fasciata confluens were investigated and the results were compared with similar studies on Nerodia rhombifera rhombifera and Elaphe obsoleta obsoleta (Baeyens et al., 1978). In addition, morphological and hematological parameters contributing to underwater survival were examined. The duration of underwater survival for N. erythrogaster and N. fasciata was approximately one hour with no difference between the species. The lung volumes of the two species were also similar but were significantly less than lung volumes reported for E. obsoleta. There were no significant differences in hemoglobin concentration, red blood cell count or hematocrit between N. rhombifera, N. erythrogaster, N. fasciata, and E. obsoleta. Based on similarities in underwater tolerance, lung morphology and hematology, Nerodia more closely resembles the terrestrial E. obsoleta than those reptiles specifically adapted to an underwater existance

Some Physiological and Morphological Adaptations for Underwater Survival in Natrix rhombifera and Elaphe obsoleta

The submergence times of the diamondback water snake (Natrix rhombifera) and black rat snake (Elaphe obsoleta) were compared. Both species could easily survive underwater for periods greater than one hour. Furthermore, there was no difference in time of underwater survival in the two species. Some physiological and morphological parameters which may contribute to the ability of N. rhombifera and E. obsoleta to remain submerged were also examined. E. obsoleta was found to have a greater lung volume and larger and more numerous alveoli than N. rhombifera. Both species demonstrated a bradycardia upon submergence but it was less pronounced than the bradycardia of the true diving animals. It is concluded that N. rhombifera has few physiological adaptations for diving and that some of the physiological attributes for an aquatic existence have already developed in E. obsoleta

A Whole-Genome RNA Interference Screen Reveals a Role for Spry2 in Insulin Transcription and the Unfolded Protein Response.

Insulin production by the pancreatic β-cell is required for normal glucose homeostasis. While key transcription factors that bind to the insulin promoter are known, relatively little is known about the upstream regulators of insulin transcription. Using a whole-genome RNA interference screen, we uncovered 26 novel regulators of insulin transcription that regulate diverse processes including oxidative phosphorylation, vesicle traffic, and the unfolded protein response (UPR). We focused on Spry2-a gene implicated in human type 2 diabetes by genome-wide association studies but without a clear connection to glucose homeostasis. We showed that Spry2 is a novel UPR target and its upregulation is dependent on PERK. Knockdown of Spry2 resulted in reduced expression of Serca2, reduced endoplasmic reticulum calcium levels, and induction of the UPR. Spry2 deletion in the adult mouse β-cell caused hyperglycemia and hypoinsulinemia. Our study greatly expands the compendium of insulin promoter regulators and demonstrates a novel β-cell link between Spry2 and human diabetes

Erratum to: Design of pharmaceutical products to meet future patient needs requires modification of current development paradigms and business models

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Onderzoek naar de trekvissoorten in het Schelde-estuarium. Voortplantings-en opgroeihabitat van rivierprik en fint

Migratory fish such as river lamprey and twaite shad are important indicators of ecosystem functioning. Over the past century, most migratory fish have disappeared from the river Scheldt due to human impacts. The previous study on migratory fishes in the Scheldt showed however that most species show the first signs of recovery (Stevens et al., 2009). For both river lamprey and twaite shad there are strong indications that they reproduce in the Scheldt. However, the spawning and nursery habitats of both species are unknown and it is unclear whether the preconditions for a sustainable recovery are met. The spawning and nursery habitat of river lamprey can be located through targeted sampling of the larvae in the sediment. Sampling with fyke nets showed that adult river lamprey migrate mainly to the Bovenschelde and Zwalmbeek. In both rivers a number of locations were selected, which are, according to the literature, expected to be suitable habitats for the larvae of river lamprey. Wadable sites were sampled with a specially designed sediment pump and the deeper sites with a Van Veen grab. In neither of these rivers, however, river lamprey larvae could be found and no spawning sites could be identified. Possible reasons for the lack of larvae in the samples are (1) that no suitable larval habitat is present in the studied areas, (2) that the larval density in the investigated habitats is low and hence sampling frequency should be increased, (3) that the River Bovenschelde and the River Zwalm are not the main spawning grounds for river lamprey in the Scheldt. Telemetry of adult river lamprey could be a possible solution to locate the spawning grounds. In order to improve the reproduction and survival of river lamprey in the River Bovenschelde, the migration barriers in the Scheldt and its tributaries should be cleared and sufficient larval habitat should be availability. Larval habitat could be created in the River Zwalm and other tributaries through the restoration of natural banks. In addition, mud and sand banks in the Bovenschelde should be protected as much as possible as potential larval habitat.The population of twaite shad in the Scheldt is too small to identify the critical habitats by sampling in the field. Therefore, a habitat suitability model for spawning and larval shad was constructed based on literature data. Hereto, we first selected the environmental variables that determine habitat suitability. Next, for each variable the tolerance range was determined. Finallly, the variables were combined using fuzzy logic in order to determine the degree of suitability of a habitat. The model predicts the presence of suitable spawning habitat in the Upper Zeeschelde, upstream of the River Durme. Later in the season, when the water temperature rises, suitable spawning habitat is also present in the Rivers Kleine Nete and Grote Nete. Suitable habitat for larval shad is located mainly in the Upper Zeeschelde upstream Rupelmonde and in the River Rupel. Spawning of twaite shad takes place in the main channel and during their ontogeny the larvae migrate to the edges of the main channel and to side channels.Therefore, in areas with suitable spawning and larval habitat, both the main and side channels need protection. In particular mudflats, sand flats and subtidal low dynamic habitats should be safeguarded. Dredging of these habitats thus mortgages the recovery of the twaite shad population in the Scheldt. The oxygen concentration in the estuary has been greatly improved in recent years.However, in summer a low-oxygen zone in the freshwater area persists, comprising the upstream migration of adults and the survival of larvae. Periodic hypoxic conditions should therefore be avoided and a minimum oxygen content of 5 mg / l is essential for both adults and larvae. During the last century, hydrodynamics in the estuary has increased markedly. As a result, larvae have more difficulties in maintaining their position in suitable habitat. Actions that increase the river/tidal flow or eliminate local retention areas should therefore be avoided