NTI-ULCEROGENIC EFFECT OF GENISTEIN AGAINST INDOMETHACIN-INDUCED GASTRIC ULCER IN RATS

ABSTRACTObjectives: Free radicals generation, inflammation, and nitric oxide (NO) modulation are involved in indomethacin (IND)-induced gastric ulcers.Most of the drugs used for the treatment of gastric ulcer have various side effects. Genistein (GEN), the natural isoflavones isolated from soya beanhas an antioxidant, anti-inflammatory, and NO modulating activity. These properties could make GEN a promising and safe, natural candidate againstIND-induced peptic ulcers.Methods: Rats were divided into four groups. Control group; GEN group (10 mg/kg, p.o.); IND group (48 mg/kg, orally); and GEN+IND group (GENadministered 1 week before IND injection). 6 hrs after IND administration, all rats were sacrificed. Gastric juice acidity and gastric injury wereevaluated directly. The levels of tumor necrosis factor alpha (TNF-α), myeloperoxidase (MPO), and NO were determined in gastric tissues. Moreover,glutathione (GSH), malondialdehyde (MDA), and superoxide dismutase (SOD) activity were determined in mucosal tissues.Results: Several mechanisms are involved in IND-induced ulcers as evidenced from the increase in TNF-α, MPO, and reduction in NO levels. In addition,free radicals also participate in the pathogenesis as evidenced from the increase in MDA levels and reduction in GSH levels and SOD activity. On theother hand, pre-administration of GEN markedly attenuated IND-induced ulcers without affecting gastric acidity, through a reduction of the elevatedTNF-α and MPO levels. Moreover, GEN significantly restored the declined NO level and ameliorated the unbalanced oxidative stress parameters.Conclusion: GEN markedly protects against IND-induced ulcers as revealed from biochemical data and amelioration of IND-induced lesions.Therefore, GEN may be a promising candidate for protection against IND-induced gastropathy.Keywords: Genistein, Ulcers, Indomethacin, Nitric oxide, Myeloperoxidase, Tumor necrosis factor alpha

Glycemic control and anti-osteopathic effect of propolis in diabetic rats

The aim of the study was to explore the possibility that propolis can control diabetes mellitus and prevent diabetic osteopathy in rats. The study compared 60 streptozotocin (STZ)-induced diabetic rats, with ten nondiabetic rats used as a negative control. The experimental design comprised seven groups (n = 10 rats per group): (1) nondiabetic, used as a negative control; (2) nontreated, used as a positive control; (3) treated with insulin alone; (4) treated with a single dose of propolis alone; (5) treated with a double dose of propolis; (6) treated with insulin and a single dose of propolis; and (7) treated with insulin and a double dose of propolis. After 6 weeks of treatment, the rats were sacrificed. Ratios of femur ash to femur weight and of femur weight to body weight (FW/BW) were calculated and calcium (Ca), phosphorus (P), and magnesium (Mg) concentrations in femur ash were estimated and analyzed. Fasting blood glucose (FBG), plasma insulin and glucagon, serum thiobarbituric acid reactive substances (TBARS), plasma parathyroid hormone (PTH), and calcitonin levels were also estimated and analyzed. There was significant reduction in FBG in all diabetic treated rats. Similarly, higher plasma insulin levels were observed in diabetic rats treated with propolis and insulin than in nontreated diabetic rats, although plasma insulin was not comparatively higher in diabetic rats treated with insulin alone. Serum TBARS was significantly lower in the propolis treated rats than the diabetic nontreated rats. No differences in PTH and calcitonin levels were observed among treatment groups. The FW/BW ratio was significantly higher in diabetic treated groups than in control groups. Furthermore, diabetic rats treated with propolis and insulin had significantly higher Ca, P, and Mg concentrations in femoral ash than nontreated diabetic rats and diabetic rats treated with insulin alone. In conclusion, propolis has a remarkable effect on glucose homeostasis and bone mineralization

The Effect of Heat Stress on Blood Picture of Japanese Quail

This study aimed to evaluate the effect of heat stress on quail hematological parameters. A total number of 300 Japanese quail chicks (Coturnix Coturnix japonica) of 28 days old were exposed to different levels of raising temperatures (22, 29, 34, 36 and 40 ºC) for 15 days. At 42 days old, blood samples were collected from 14 birds (7 males + 7 females) that were randomly selected. The samples were used for determining red blood cells count, hemoglobin concentration, packed cell volume, total and differential white blood cells count, heterophils/ lymphocyte (H/L) ratio, mean corpuscular hemoglobin, mean corpuscular volume and mean corpuscular hemoglobin concentration. The obtained results indicated that, rearing quail birds under high environmental conditions during fattening period has a great adverse effect on these blood parameters

Amelioration of The Dielectric Properties of Ceramic Insulators Using Nano-alumina

Outdoor HV porcelain insulators face various environmental stresses that cause their degradation. Consequently, amelioration of their insulating properties becomes a target of recent researches to survive higher voltage levels. Investigating the impact of the addition of 0, 5, 10, and 15 wt. % nano- alumina (NA) on the dielectric and physical characteristics of porcelain materials at elevated sintering temperatures is the aim of this study. Porcelain specimens were synthesized from kaolin, feldspar, and quartz as available low-cost raw materials. The specimens were sintered at 1100, 1200, 1300, and 1400°C for 2 h. For some specimens, the microstructure and phases formed were identified using scanning electron microscopy and X-ray diffraction techniques. The changes that occur upon heating (include melting, phase transition, sublimation, and decomposition) were identified by Differential Thermogravimetric Analysis. The dielectric strength, relative permittivity, and loss tangent of different samples were measured at a large scale of frequencies. Breakdown strength values of different samples were verified by applying the Finite Element Method. The best electrical and physical properties were achieved at 1300ºC. At this temperature the porcelain sample containing 5 wt. % NA presented optimum physical characteristics as well as good insulating properties assent the feasibility of producing electro-technical porcelai

The effect of serum angiotensin II and angiotensin II type 1 receptor gene polymorphism on pediatric lupus nephritis

Background: Angiotensin II (Ang II) is found to perpetuate inflammation and visceral damage in systemic lupus erythematosus (SLE). It mediates most of its actions through Ang II receptor type I (AT1) whose gene polymorphism A1166C (CC genotype) seems to have pathogenic effects. Objective: To measure serum Ang II and the frequency of AT1 receptor CC genotype among a group of Egyptian patients with pediatric onset lupus nephritis (pLN). Methods: This is a case-control cross sectional study which included 24 patients with pLN and 24 age and sex-matched healthy subjects as controls. Clinical evaluation and routine laboratory markers for SLE patients were done. SLE disease activity index (SLEDAI) and the British Isles Lupus Assessment Group (BILAG)-2004 renal score were measured. Serum Ang II was measured by enzyme linked immunosorbent assay and detection of ATI receptor CC genotype by polymerase chain reaction were done for both patients and controls. Results: Patients had significantly higher serum Ang II than the controls (p=0.0001). The frequency of AT1 receptor CC genotype was significantly higher among patients as compared to the control group (p=0.008). Both serum Ang II and AT1 receptor CC genotype were comparable between patients with proliferative LN class III and IV and those with LN class II (p>0.05). Serum Ang II did not correlate significantly with SLEDAI or BILAG-renal score (p>0.05). Conclusion: Serum Ang II and AT1 receptor CC genotype seem to have pathogenic role in pLN but with no deleterious effects on the phenotype of LN for further assessment.Keywords: Lupus nephritis; Angiotensin II; Angiotensin II type 1 receptor; Polymorphism; Pediatrics

Cosmological applications in Kaluza-Klein theory

The field equations of Kaluza-Klein (KK) theory have been applied in the domain of cosmology. These equations are solved for a flat universe by taking the gravitational and the cosmological constants as a function of time t. We use Taylor's expansion of cosmological function, $\Lambda(t)$, up to the first order of the time $t$. The cosmological parameters are calculated and some cosmological problems are discussed.Comment: 14 pages Latex, 5 figures, one table. arXiv admin note: text overlap with arXiv:gr-qc/9805018 and arXiv:astro-ph/980526

Influence of Different Decontamination Approaches on Bone Substitute Adhesion to Peri-Implantitis Affected Implant Surfaces: An SEM Proof of Principle Study

Background: During healing, clot blended graft materials may retract away from implant surfaces creating microgaps that compromise re-osseointegration. The present study aimed to evaluate different surface decontamination materials’ effect on adhesion of the graft materials to peri-implantitis affected parts, a factor that can resist clot blended graft retraction improving re-osseointegration. Methods: Eighteen peri-implantitis affected implants diagnosed as hopeless and designated for removal contributed in this prospective, masked trial. Samples were randomly distributed into three groups, each of six implants. Group one (G1) was coated with hydroxyapatite of a micro particle size of 250 to 1000 µm after saline surface decontamination for two minutes. Group two (G2) peri-implantitis affected parts were treated with the graft material following two minutes of chlorhexidine gluconate 0.12% (CHX) surface treatment. Group three (G3) implants were coated with the graft material after citric acid (CA) (pH = 1) surface conditioning for two minutes. Implants in all groups were agitated in phosphate-buffered saline (PBS) by using an automatic tissue processor agitator for three minutes. Implants were prepared for surface scanning evaluation. Results: Scanning electron microscopy (SEM) observation of G1 saline treated control implants were devoid of bone particles adherent to peri-implantitis affected surfaces. The surface area covered by grafted particles in G2 was statistically higher than that of G1 (P<0.01). Group three (CA-treated) showed nearly complete coverage of peri-implantitis affected parts by the graft material covering 88.8% of examined surface areas which was statistically higher than that of G2 (P<0.05). Conclusion: Citric acid implant surface conditioning could improve implant re-osseointegration through enhancement of the graft adhesion to the implant surface. Smear layer barrier effect seemed to be the most important factor that compromised graft adhesion to preri-implantitis affected parts of the implant surfaces

Typical behavior of relays in communication channels

The typical behavior of the relay-without-delay channel under low-density parity-check coding and its multiple-unit generalization, termed the relay array, is studied using methods of statistical mechanics. A demodulate-and- forward strategy is analytically solved using the replica symmetric ansatz which is exact in the system studied at Nishimori's temperature. In particular, the typical level of improvement in communication performance by relaying messages is shown in the case of a small and a large number of relay units. © 2007 The American Physical Society

Neutrophil functions in late preterm neonates with respiratory distress syndrome

Background: Studies that have addressed the effects of respiratory distress syndrome (RDS) on neutrophil function suggested that neutrophil functions other than the generation of the respiratory burst are not impaired. Yet, results have been confusing and in some cases contradictory.Objectives: The aim of this cross-sectional controlled study is to assess neutrophil number and function in late preterm neonates with RDS.Methods: Thirty patients underwent clinical and laboratory evaluation including complete blood counts and tests of neutrophil functions (CD11b, CD62L and Dihydrorhodamine 123 by flowcytometry) in comparison to 15 healthy term controls. RDS was assessed clinically and radiologically (chest x-ray).Results: Fifty percent of patients (12 females and 18 males) had grade II respiratory distress followed by grade III then grade I. DHR, CD 11b and CD62L results were lower among the patients group (mean ± SD: 62.1± 12.23, 63.22 ± 11.41, 15.03 ± 8.7 respectively). There were no significant correlations between neutrophils count, DHR, CD11b and CD62L. Only CD11b was significantly lower with higher grades of RDS.Conclusion: Neonates with RDS show variable affection of neutrophil functions. Further studies are recommended to elucidate the exact mechanisms by which RDS can affect neutrophil functions and whether these effects are associated with increased incidence of infections.Keywords: Neutrophils, function, respiratory distress syndrome, late preterm, innate immunity, infections, adhesion molecule