Genetic Diversity of Some Sweet Cherry Cultivars Based on Molecular Markers

Sweet cherry (Prunus avium L.), originated around the Caspian and Black Sea, is an important fruit tree species of economic interest, and hence, breeding and conservation are requested (. Genetic analysis at the molecular level can be used effectively to study molecular polymorphism existing between intraspecific and interspecific tree species and phylogenetic relationships between them and their hybrids. The purpose of this study was to characterize and determine genetic relationships among the sweet cherry native genotypes belonging to Fruit Research & Development Station Bistrita, Romania, using RAPD markers. To eliminate the existence of possible synonyms from national romanian collection, we collect four Van cultivars, from four different national collection. For molecular analysis of the 16 varieties of sweet cherry were considered 13 RAPD primers selected from the literature. They were later used to determine the genetic variability at the molecular level using PAST program, and the dendrogram was generated based on Jaccard’s genetic distance. The dendrogram constructed by PAST software. The quantity and quality of the DNA obtained was suitable to achieve PCR amplification step. Only seven out of the 13 RAPD primers have generate polymorphic bands. The rest of seven were monomorphics. The most polymorphic primer was OPB10 which generated 11 bands from which 100% were polymorphic.Seven RAPD primers generated a high level of polymorphism which allowed to divide these cherry varieties into two groups according to their genetic geographical origin and the pedigree

Accuracy of 2D Fluoroscopy with Preoperative CT Fused Neuronavigation in Thoracic and Lumbar Pedicle Screw Insertion

Aim: Pedicle screw fixation is an established technique in the lumbar and thoracic area. Fluoroscopy-guided screw placement and subsequently navigation have decreased the rate of misplaced screws, but no technique has wholly eliminated this risk. This paper aims to study the difference between the accuracy of the fluoroscopic guided screw placement to that of the 2D fluoroscopy- preop CT fused neuronavigation guided technique, a lesser-used navigation technique.  Material and Methods: This retrospective study reflects our results using both techniques between March 2018 and March 2019 in both degenerative or traumatic spinal pathology for thoracic and lumbar regions. The accuracy of the screw placement was measured using Mirza grading system on postoperative CT images. Results: A total number of 56 patients underwent spinal instrumentation surgery. A total of 274 screws were placed with a mean number of 4.89 screws per patient; 199 screws were implanted using neuronavigation and 75 using the freehand-2D fluoroscopy-guided technique.  The accuracy rate of pedicle screw placement in the freehand technique guided by 2D fluoroscopy was 88,00%. With the use of neuronavigation, the accuracy increased to 89,96%. Conclusion:  Pedicle screw placement accuracy is higher when guided by CT-fluoro matching neuronavigation compared to freehand fluoroscopy-guided technique and can be used in departments where there is no intraoperative O-arm or 3D fluoroscopy available

ANALYSIS OF THE INFLUENCE OF FINANCIAL EQUILIBRIUM ON THE PROFITABILITY OF TOURISM COMPANIES

The financing of the activity represents an important part of the company's financial policy. The working capital represents the resources that the company needs to finance its current production activity. Maintaining a balance between working capital and needs is a goal that supports the smooth running of the activity, which suggests that financial balance influences profitability, so the purpose of this paper is to verify this. In this sense, 20 tourism companies listed on the Bucharest Stock Exchange were selected, using a data set for the period 2008-2022, processed through regression analysis. The results obtained indicate a correlation between the net profit margin (dependent variable) and the working capital ratio (independent variable). Also, the dependent variable is explained to the extent of 46.4% by the independent variables

IMPLICATIONS OF FINANCIAL STRUCTURE ON THE VALUE OF COMPANIES FROM TOURISM INDUSTRY

The financial structure highlights the origin of the capital needed to finance the activity. Basically, it indicates the extent to which the company opts for equity and debt financing. It is difficult or even impossible to identify an optimal capital structure that is efficient for all firms in all fields of activity. Thus, the main objective of this paper is to identify the influence of the financial structure on the value of companies in the tourism industry. The value of a company is reflected by the efficiency of the activity it carries out. To the extent that it manages to obtain a higher profit, increase the value of the patrimony or use the resources at its disposal more efficiently, its value can be considered to increase. Another objective is to identify if during the pandemic the companies in the tourism industry changed their financial structure and also if during this difficult period there was a change in the influence that the financial structure has on the value of the company. The study carried out on the basis of information on 20 companies in the tourism industry, for the period 2008-2019 and 2020-2022, regarding the correlation between total liabilities, equity, leverage and the EPS (net profit/share) indicator, highlighted an impact moderate of the financial structure on the value of the company during the pandemic period

Revisiting the Organisational Identification Assessment from a managerial perspective: A case study on a Romanian public University

Although there is a large body of literature on organizational identification (OID), in recent years few of them have addressed the higher education context, mostly on student’s identification and rarely on the identification of academic and research staff. Of these, their main focus is usually on different constructs, exploring OID in its role as mediator/moderator of these organizational issues and most often only one form of OID is acknowledged. The theoretical progress is evident, the essential necessity for large organizations to foster a sense of oneness in employees is acknowledged. However, from a managerial perspective, the issues of how many or which antecedents should be nurtured in this context in order to obtain results and the doubtful adaptability of considering just one form of OID are almost a decade old. This paper presents and discusses the results of a survey carried out on 1072 academics and researchers from Babes-Bolyai University, Romania. By assessing three forms of OID (OID, ambivalent identification and neutral identification) and certain relevant antecedents in the case of a large public university, the study addresses a gap in recent literature. Results are consistent with the Extended Model of Organizational Identification and strengthen the need to acknowledge the operational importance of ambivalent and neutral identification from a managerial perspective in the case of higher education employees. Theoretical contributions and practical implications of results are also discussed

EMG signal processing for the study of localized muscle fatigue—pilot study to explore the applicability of a novel method

This pilot study aimed to explore a method for characterization of the electromyogram frequency spectrum during a sustained exertion task, performed by the upper limb. Methods: Nine participants underwent an isometric localized muscle fatigue protocol on an isokinetic dynamometer until exhaustion, while monitored with surface electromyography (sEMG) of the shoulder’s external rotators. Firstly, three methods of signal energy analysis based on primer frequency contributors were compared to the energy of the entire spectrum. Secondly, the chosen method of analysis was used to characterize the signal energy at beginning (T1), in the middle (T2) and at the end (T3) of the fatigue protocol and compared to the torque output and the shift in the median frequencies during the trial. Results: There were statistically significant differences between T1 and T3 for signal energy (p < 0.007) and for central frequency of the interval (p = 0.003). Moreover, the isometric peak torque was also different between T1 and T3 (p < 0.001). Overall, there were no differences between the signal energy enclosed in the 40 primer frequency contributors and the analysis of the full spectrum energy; consequently, it was the method of choice. The reported fatigue and the decrease in the produced muscle torque was consistent with fatigue-induced alterations in the electromyogram frequency spectrum. In conclusion, the developed protocol has potential to be considered as an easy-to-use method for EMG-based analysis of isometric muscle exertion until fatigue. Thus, the novelty of the proposed method is to explore, in muscle fatigue, the use of only the main contributors in the frequency domain of the EMG spectrum, avoiding surplus information, that may not represent muscle functioning. However, further studies are needed to investigate the stability of the present findings in a more comprehensive sample.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CeDRI (UIDB/05757/2020 and UIDP/05757/2020) and SusTEC (LA/P/0007/2021).info:eu-repo/semantics/publishedVersio

Studiul acțiunii antioxidante a unor plante medicinale din colecția CȘCPM USMF ,,Nicolae Testemițanu” prin utilizarea testului DPPH.

Interesul în antioxidanţi naturali, în special, cei proveniţi din plante medicinale, a crescut în ultimii ani, inclusiv prin multiple studii și publicații. Scopul acestei lucrări constă în confi rmarea acțiunii antioxidante, cu utilizarea metodei spectrofotometrice prin testul 1,1,-difenil-2-picrilhidrazil (DPPH), pentru unele produse vegetale: Argimoniae herba, Cichorii herba, Potentillae herba, Symphyti radix, colectate din colecția Centrului Științifi c de Cultivare a Plantelor Medicinale USMF ,,Nicolae Testemițanu”. Rezultatele experimentale obţinute denotă că părțíle aeriene de turiță manifestă cea mai înaltă activitate antioxidantă, urmată de sclipeți, cicoare și tătăneasă: Agrimonia eupatoria L. (IC 50 = 45.557 μg/ml) ˃ Potentilla erecta L. Rausch. (60.647 μg/ml) ˃ Cichorium intybus L. (IC 50 = 173.08 μg/ml) ˃ Symphytum offi cinale L. (205,56 μg/ml)