Production of lamp cover

Projekt predkladá návrh výroby krytu osvetlenia z ocele 11 320 v sérii 43 000. Po zhodnotení všetkých možností ako súčasť vyrobiť, bola zvolená metóda hlbokého ťahania konvenčným nástrojom. Súčasť bude vyrobená na jednu operáciu. Konštrukcia nástroja využíva, ako normalizovaných súčiastok, tak aj častí vyrábaných. Ten je upnutý v hydraulickom lise CTC 25. Ťažník a ťažnica sú vyrobené z nástrojovej oceli 19 191. Orientačne boli stanovené výrobné náklady 15,21 Kč/ks a bolo zistené, že od 26 173 vyrobených kusov výroba prestane byť stratová.Project dissertate about manufacturing of lamp cover of steel 13 200 in serie 43 000. After comparing all the alternatives how to make the product , was choose a method of convention deep drawing. Product is manufactured in one operation. Design of apparatus using as standardized, so special parts. It is fitted in hydraulic pressing machine CTC 25. Functional parts are made from tool steel 19 191. Costs of production was calculated on 15,21 crowns a piece. From 26 173 manufactured pieces will the production gainful.

Transthyretin in the Evaluation of Health and Disease in Human and Veterinary Medicine

Transthyretin (also known as prealbumin) is an important transport protein, which plays an essential role in the binding of thyroid hormones and retinol with varying affinities in mammalian, as well as avian species. The determination of transthyretin concentrations may be used as a diagnostic tool for some disease conditions in humans, but is more often used as a nutritional marker to assess protein-calorie malnutrition and as prognostic indicator in critically ill patients. Transthyretin has shorter half-life (2–3 days) than that of albumin and belongs to negative acute phase proteins. This may complicate the use of transthyretin as a nutritional marker and the interpretation of results in the diagnosis of diseases. Although some studies have been carried out to determine the usefulness of transthyretin in selected disease conditions and disorders also in animals, it is a relatively rarely used parameter to evaluate health state and illness in veterinary medicine. The usefulness of transthyretin in the diagnosis of diseases and evaluation of nutritional status in humans and animals are reviewed in this article, including the laboratory assays available to measure its concentrations and the possible clinical application of the results, as well as its usefulness as a prognostic indicator in some disease conditions

Comparison of growth parameters of 40 varieties/clones short rotation coppice willow (Salix)

The plantation of willow varieties was established in 2009. The 40 different varieties and clones of Salix were planted at a research field in Kolíňany (Nitra district). The paper evaluates results from the first growing season in 2009. The survival rate of planted cuttings ranged from 55.56 % to 100.00 % after the first year. The lowest survival rate was reached by Terra Nova variety. The stem numbers per plant ranged from 1.17 ±0.37 to 2.53 ±0.98. The average height of one-year old stem varied from 65.82 ±36.60 cm to 225.58 ±68.61 cm. The average stem diameter ranged from 6.90 ±2.63 mm to 14.34 ±3.39 mm. There were statistically very significant differences in parameters of stem height stem diameter and stem numbers per plant among studied varieties/clones. The statistic method used was analysis of variance ANOVA. The varieties were then divided into 6 groups according to their similarity in observed parameters after the first growing season by cluster analysis. The best results were reached by varieties/clones classified in the second group. The survival rate, stem diameter and stem height values of these varieties/clones were above average.&nbsp

OCCURENCE OF FUSARIUM HEAD BLIGHT OF WHEAT IN SLOVAKIA UNDER THE NATURAL INFECTION

Occurrence of Fusarium head blight (FHB) was documented during two consecutive years in June 2011-2012 under the natural conditions in winter wheat (Triticum aestivum L.). Observations were conducted at six different localities in four climatic regions in Slovakia. Incidence and severity of FHB were evaluated at the end of flowering stage in three replications. Each replication contained 100 spikes. These data served as a basis for FHB index calculations. Obtained FHB index values indicated that the environmental conditions of the year 2011 were more favourable to the development of FHB infection. Higher FHB index values were reached at localities with precipitation higher than 100% of long-term average. Although significantly higher incidence of heads with FHB symptoms was recorded in climatic region 02 – quite warm, dry, hilly, correlation between the climatic regions was not confirmed. Except of the climatic conditions, the FHB development can be influenced by nitrogen application. The highest levels of FHB index was in coincidence with the highest and the lowest nitrogen rates applied. In all other cases, the effect of the mineral nutrition on head blight attack was unclear. Analyses of nitrogen forms applied revealed that nitrogen forms had no impact on FHB index value

Use of wood ash for fertilizing the plantation of fast-growing woody plants-basket willow (Salix viminalis L.)

Energetic utilization of wood biomass obtained from fast-growing woody plants nowadays records more important capability. The article discusses the issue of research using wood ash as solid waste from the boiler while burning biomass and the intensification of short-rotation coppice basket willow - common osier (Salix viminalis L.) for energy use as a renewable energy source. Purpose of the article is to show the importance of basket willow (Salix viminalis L.) as short-rotation coppice with the emphasis of its production and remedial effects

Spa industry resilience in changing external conditions: Nexus between tourist attractiveness and fiscal policy

Purpose – This study aims to evaluate the performance of the Czech spa industry within a dynamically changing external environment, specifically in the context of the COVID-19 pandemic. Considering the relationship between tourist attractiveness and fiscal policy effectiveness, the research explores the divergences existing among spa companies functioning across discrete spa resort categories within the Czech Republic. Methodology/Design/Approach – The research employs secondary data obtained from the State Statistical Office and the Business Register. The analysis entails an examination of the relationship between the level of tourist attractiveness and alterations in sales. Findings – The study reveals that spa resorts with higher levels of tourist attractiveness encountered a more pronounced decline in sales. Despite the notable allocation of public funds, the impact of subsidies on economic outcomes remained limited. Originality of the research – A contribution lies in its revelation of the adverse implications of over-reliance on tourism for the spa industry. The study highlights the imperative for diversification in resort portfolios. Moreover, the research offers valuable insights into the optimal allocation of subsidies, suggesting directions for more effective utilization and distribution in a manner that supports the industry\u27s long-term sustainability and resilience

The Use of Serum Proteins in the Laboratory Diagnosis of Health Disorders in Ruminants

Although hundreds of proteins exist in blood serum, little is known about the precise composition and entire set of serum proteins in different ruminant species. Under physiological conditions, the production of serum proteins is closely regulated, but alterations in the serum protein pattern may occur in a wide range of diseases and health disorders. During the last several years, substantial progress was seen in the application of serum protein analyses for diagnostic purposes. The serum protein profile is mostly evaluated by serum protein electrophoresis, which allows the identification of protein fractions, each being composed of several individual proteins with similar electrophoretic mobility. Many disease processes can cause changes in the concentrations of serum proteins. Therefore, the determination of their concentrations and the evaluation of changes in their concentrations during the disease process may provide important diagnostic information for assessing the health state. Despite this usefulness, the evaluation of serum protein pattern is still relatively a less frequently used laboratory diagnostic technique in ruminant medicine. Thus, the usefulness of serum proteins in the diagnosis of health disorders and the possible clinical application of the results of the electrophoretic separation of serum proteins in ruminants will be reviewed in this chapter

Enzýmová hydrolýza α-substituovaných akrylonitrilov

Cilem diplornové préce bylo ovéiit schopnost kmene A4 néleiejiciho do rodu Rhodococcus transformovat chirélni on-substitutovane' akrylonitrily, které jsou prekursory stavebnich blokfi pro syntetickou organickou chemii. Zminénjz kmen produkoval komplex enzymfi - nitrilhydratasu a amidasu -- umoifiujici transformaci nitrilfi na karboxylové kyseliny s amidy jako intermediéty. Transformace oc-substitutovanjrch akrylonitrilfi byla provedena pomoci celjzch bunék této baktérie nebo éésteéné purifikované nitrilhydratasy. Reakéni produkty byly izolovény a identifikovény pomoci spektrélnich metod jako amidy, kyseliny nebo lakton. Koncentrace substrétfi a produktfi V reakénich smésich byly sledovémy pomoci HPLC na reverzni fézi. Pro tento fiéel byly vypracovény originélni metody, podobné jako methody chirélni I-IPLC, které byly vyuiity ke stanoveni enantiomerni éistoty chirélnich produktfi. Katalyzétory obsahujici celé bufiky nebo éésteéné purifikovanou nitrilhydratasu byly vhodné pro piipravu wsubstitutovanjrch akrylamidfi a akrylovjzch kyselin, z nichi nékteré jsou intermediéty pro syntézu biologicky aktivnich létek.The aim of the diploma work was to examine the ability of strain A4 belonging to Rhodococcus to transform chiral on-substituted acrylonitriles, which are the precursors of building blocks for synthetic organic chemistry. The aforementioned strain produced a complex of enzymes - nitrile hydratase and amidase - enabling to transform nitriles into carboxylic acids with amides as intermediates. The transformation of ot-substituted acrylonitriles were carried out using whole cells of the bacterium or the partially purified nitrile hydratase. The reaction products were isolated and identified by spectral methods as the corresponding amides, acids or a lacton. The concentrations of the substrates and products in the reaction mixtures were monitored by reversed-phase HPLC. Original HPLC methods were worked out for this purpose, as well as the chiral HPLC methods, which were used to determined the enantiomeric purity of the chiral products. The catalysts consisting of whole cells or the partially purified nitrile hydratase proved to be suitable for the preparation ofon-substituted acrylamides and acrylic acids, some ofwhich are intermediates for the synthesis ofbiologically active compounds.Katedra biochemieDepartment of BiochemistryFaculty of SciencePřírodovědecká fakult