Perinatal Derivatives: Where Do We Stand? A Roadmap of the Human Placenta and Consensus for Tissue and Cell Nomenclature

Progress in the understanding of the biology of perinatal tissues has contributed to the breakthrough revelation of the therapeutic effects of perinatal derivatives (PnD), namely birth-associated tissues, cells, and secreted factors. The significant knowledge acquired in the past two decades, along with the increasing interest in perinatal derivatives, fuels an urgent need for the precise identification of PnD and the establishment of updated consensus criteria policies for their characterization. The aim of this review is not to go into detail on preclinical or clinical trials, but rather we address specific issues that are relevant for the definition/characterization of perinatal cells, starting from an understanding of the development of the human placenta, its structure, and the different cell populations that can be isolated from the different perinatal tissues. We describe where the cells are located within the placenta and their cell morphology and phenotype. We also propose nomenclature for the cell populations and derivatives discussed herein. This review is a joint effort from the COST SPRINT Action (CA17116), which broadly aims at approaching consensus for different aspects of PnD research, such as providing inputs for future standards for the processing and in vitro characterization and clinical application of PnD.Austrian Science Fund (FWF) DOC 31-B26Medical University GrazUniversita Cattolica del Sacro CuorePRIN 2017 program of Italian Ministry of Research and University (MIUR) 2017RSAFK7Ministry of Health, Italy GR-2018-12366992Slovenian Research Agency - Slovenia P3-0108MRIC UL IP-0510Plan Estatal de Investigacion Cientifica y Tecnica y de InnovacionISCIII Subdireccion General de Evaluacion y Fomento de la InvestigacionMinisterio de Economia y Competitividad, Spain PI16/01642European Union (EU)European Community (EC)German Research Foundation (DFG) GE-2223/2-

PlGF Repairs Myocardial Ischemia through Mechanisms of Angiogenesis, Cardioprotection and Recruitment of Myo-Angiogenic Competent Marrow Progenitors

Despite preclinical success in regenerating and revascularizing the infarcted heart using angiogenic growth factors or bone marrow (BM) cells, recent clinical trials have revealed less benefit from these therapies than expected.We explored the therapeutic potential of myocardial gene therapy of placental growth factor (PlGF), a VEGF-related angiogenic growth factor, with progenitor-mobilizing activity.Myocardial PlGF gene therapy improves cardiac performance after myocardial infarction, by inducing cardiac repair and reparative myoangiogenesis, via upregulation of paracrine anti-apoptotic and angiogenic factors. In addition, PlGF therapy stimulated Sca-1(+)/Lin(-) (SL) BM progenitor proliferation, enhanced their mobilization into peripheral blood, and promoted their recruitment into the peri-infarct borders. Moreover, PlGF enhanced endothelial progenitor colony formation of BM-derived SL cells, and induced a phenotypic switch of BM-SL cells, recruited in the infarct, to the endothelial, smooth muscle and cardiomyocyte lineage.Such pleiotropic effects of PlGF on cardiac repair and regeneration offer novel opportunities in the treatment of ischemic heart disease

Measurement of Hadron Production in π−\pi^--C Interactions at 158 and 350 GeV/c with NA61/SHINE at the CERN SPS

We present a measurement of the momentum spectra of $\pi^\pm$, K$^\pm$, p$^\pm$, $\Lambda$, $\bar{\Lambda}$ and K$^{0}_{S}$ produced in interactions of negatively charged pions with carbon nuclei at beam momenta of 158 and 350 GeV/c. The total production cross sections are measured as well. The data were collected with the large-acceptance spectrometer of the fixed target experiment NA61/SHINE at the CERN SPS. The obtained double-differential $p$-$p_T$ spectra provide a unique reference data set with unprecedented precision and large phase-space coverage to tune models used for the simulation of particle production in extensive air showers in which pions are the most numerous projectiles

Measurement of hadron production in π–^{–}−C interactions at 158 and 350 GeV / c with NA61/SHINE at the CERN SPS

We present a measurement of the momentum spectra of π $^{±}$, K$^{±}$, p$^{±}$, Λ, $\overline{Λ}$, and K$^{0}$$_{S}$ produced in interactions of negatively charged pions with carbon nuclei at beam momenta of 158 and 350 GeV/c. The total production cross sections are measured as well. The data were collected with the large-acceptance spectrometer of the fixed target experiment NA61/SHINE at the CERN SPS. The obtained double-differential p−pT spectra provide a unique reference dataset with unprecedented precision and large phase-space coverage to tune models used for the simulation of particle production in extensive air showers in which pions are the most numerous projectiles

Measurements of K0^{0}S_{S}, Λ , and Λˉ\bar{Λ} production in 120 GeV / c p + C interactions

This paper presents multiplicity measurements of K0S, Λ, and ¯Λ produced in 120  GeV/c proton-carbon interactions. The measurements were made using data collected at the NA61/SHINE experiment during two different periods. Decays of these neutral hadrons impact the measured π+, π−, p and ¯p multiplicities in the 120  GeV/c proton-carbon reaction, which are crucial inputs for long-baseline neutrino experiment predictions of neutrino beam flux. The double-differential multiplicities presented here will be used to more precisely measure charged-hadron multiplicities in this reaction, and to reweight neutral hadron production in neutrino beam Monte Carlo simulations

Measurements of π+\pi^+, π−\pi^-, pp, pˉ\bar{p}, K+K^+ and K−K^- production in 120 GeV/cc p + C interactions

This paper presents multiplicity measurements of charged hadrons produced in 120 GeV/$c$ proton-carbon interactions. The measurements were made using data collected at the NA61/SHINE experiment during two different data-taking periods, with increased phase space coverage in the second configuration due to the addition of new subdetectors. Particle identification via $dE/dx$ was employed to obtain double-differential production multiplicities of $\pi^+$, $\pi^-$, $p$, $\bar{p}$, $K^+$ and $K^-$. These measurements are presented as a function of laboratory momentum in intervals of laboratory polar angle covering the range from 0 to 450 mrad. They provide crucial inputs for current and future long-baseline neutrino experiments, where they are used to estimate the initial neutrino flux

Measurements of π±\pi^\pm, K±K^\pm, pp and pˉ\bar{p} spectra in 40^{40}Ar+45^{45}Sc collisions at 13AA to 150AA GeV/cc

The NA61/SHINE experiment at the CERN Super Proton Synchrotron studies the onset of deconfinement in strongly interacting matter through a beam energy scan of particle production in collisions of nuclei of varied sizes. This paper presents results on inclusive double-differential spectra, transverse momentum and rapidity distributions and mean multiplicities of $\pi^\pm$, $K^\pm$, $p$ and $\bar{p}$ produced in $^{40}$Ar+$^{45}$Sc collisions at beam momenta of 13$A$, 19$A$, 30$A$, 40$A$, 75$A$ and 150$A$ GeV/$c$. The analysis uses the 10% most central collisions, where the observed forward energy defines centrality. The energy dependence of the $K^\pm$/$\pi^\pm$ ratios as well as of inverse slope parameters of the $K^\pm$ transverse mass distributions are placed in between those found in inelastic $p$+$p$ and central Pb+Pb collisions. The results obtained here establish a system-size dependence of hadron production properties that so far cannot be explained either within statistical (SMES, HRG) or dynamical (EPOS, UrQMD, PHSD, SMASH) models

KS0K_S^0 meson production in inelastic p+p interactions at 31, 40 and 80 GeV/c beam momentum measured by NA61/SHINE at the CERN SPS

Measurements of $K_S^0$ meson production via its $\pi^{+} \pi^{-}$ decay mode in inelastic $\textit{p+p}$ interactions at incident projectile momenta of 31, 40 and 80 GeV/$c$ ($\sqrt{s_{NN}}=7.7, 8.8$ and $12.3$ GeV, respectively) are presented. The data were recorded by the NA61/SHINE spectrometer at the CERN Super Proton Synchrotron. Double-differential distributions were obtained in transverse momentum and rapidity. The mean multiplicities of $K_S^0$ mesons were determined to be $(5.95 \pm 0.19 (stat) \pm 0.22 (sys)) \times 10^{-2}$ at 31 GeV/$c$, $(7.61 \pm 0.13 (stat) \pm 0.31 (sys)) \times 10^{-2}$ at 40 GeV/$c$ and $(11.58 \pm 0.12 (stat) \pm 0.37 (sys)) \times 10^{-2}$ at 80 GeV/$c$. The results on $K^{0}_{S}$ production are compared with model calculations (Epos1.99, SMASH 2.0 and PHSD) as well as with published data from other experiments.Comment: arXiv admin note: substantial text overlap with arXiv:2106.0753