19 research outputs found
Widespread somatic L1 retrotransposition occurs early during gastrointestinal cancer evolution
Somatic L1 retrotransposition events have been shown to occur in epithelial cancers. Here, we attempted to determine how early somatic L1 insertions occurred during the development of gastrointestinal (GI) cancers. Using L1-targeted resequencing (L1-seq), we studied different stages of four colorectal cancers arising from colonic polyps, seven pancreatic carcinomas, as well as seven gastric cancers. Surprisingly, we found somatic L1 insertions not only in all cancer types and metastases but also in colonic adenomas, well-known cancer precursors. Some insertions were also present in low quantities in normal GI tissues, occasionally caught in the act of being clonally fixed in the adjacent tumors. Insertions in adenomas and cancers numbered in the hundreds, and many were present in multiple tumor sections, implying clonal distribution. Our results demonstrate that extensive somatic insertional mutagenesis occurs very early during the development of GI tumors, probably before dysplastic growth
An Aromatic Sensor with Aversion to Damaged Strands Confers Versatility to DNA Repair
It was not known how xeroderma pigmentosum group C (XPC) protein, the primary initiator of global nucleotide excision repair, achieves its outstanding substrate versatility. Here, we analyzed the molecular pathology of a unique Trp690Ser substitution, which is the only reported missense mutation in xeroderma patients mapping to the evolutionary conserved region of XPC protein. The function of this critical residue and neighboring conserved aromatics was tested by site-directed mutagenesis followed by screening for excision activity and DNA binding. This comparison demonstrated that Trp690 and Phe733 drive the preferential recruitment of XPC protein to repair substrates by mediating an exquisite affinity for single-stranded sites. Such a dual deployment of aromatic side chains is the distinctive feature of functional oligonucleotide/oligosaccharide-binding folds and, indeed, sequence homologies with replication protein A and breast cancer susceptibility 2 protein indicate that XPC displays a monomeric variant of this recurrent interaction motif. An aversion to associate with damaged oligonucleotides implies that XPC protein avoids direct contacts with base adducts. These results reveal for the first time, to our knowledge, an entirely inverted mechanism of substrate recognition that relies on the detection of single-stranded configurations in the undamaged complementary sequence of the double helix
Extensive somatic L1 retrotransposition in colorectal tumors
L1 retrotransposons comprise 17% of the human genome and are its only autonomous mobile elements. Although L1-induced insertional mutagenesis causes Mendelian disease, their mutagenic load in cancer has been elusive. Using L1-targeted resequencing of 16 colorectal tumor and matched normal DNAs, we found that certain cancers were excessively mutagenized by human-specific L1s, while no verifiable insertions were present in normal tissues. We confirmed de novo L1 insertions in malignancy by both validating and sequencing 69/107 tumor-specific insertions and retrieving both 5âČ and 3âČ junctions for 35. In contrast to germline polymorphic L1s, all insertions were severely 5âČ truncated. Validated insertion numbers varied from up to 17 in some tumors to none in three others, and correlated with the age of the patients. Numerous genes with a role in tumorigenesis were targeted, including ODZ3, ROBO2, PTPRM, PCM1, and CDH11. Thus, somatic retrotransposition may play an etiologic role in colorectal cancer
High prevalence of germline STK11 mutations in Hungarian Peutz-Jeghers Syndrome patients
<p>Abstract</p> <p>Background</p> <p>Peutz-Jeghers syndrome (PJS) is a rare autosomal dominantly inherited disease characterized by gastrointestinal hamartomatous polyposis and mucocutaneous pigmentation. The genetic predisposition for PJS has been shown to be associated with germline mutations in the <it>STK11</it>/<it>LKB1 </it>tumor suppressor gene. The aim of the present study was to characterize Hungarian PJS patients with respect to germline mutation in <it>STK11</it>/<it>LKB1 </it>and their association to disease phenotype.</p> <p>Methods</p> <p>Mutation screening of 21 patients from 13 PJS families were performed using direct DNA sequencing and multiplex ligation-dependent probe amplification (MLPA). Comparative semi-quantitative sequencing was applied to investigate the mRNA-level effects of nonsense and splice-affecting mutations.</p> <p>Results</p> <p>Thirteen different pathogenic mutations in <it>STK11</it>, including a high frequency of large genomic deletions (38%, 5/13), were identified in the 13 unrelated families studied. One of these deletions also affects two neighboring genes (<it>SBNO2 </it>and <it>GPX4</it>), located upstream of <it>STK11</it>, with a possible modifier effect. The majority of the point mutations (88%, 7/8) can be considered novel. Quantification of the <it>STK11 </it>transcript at the mRNA-level revealed that the expression of alleles carrying a nonsense or frameshift mutation was reduced to 30-70% of that of the wild type allele. Mutations affecting splice-sites around exon 2 displayed an mRNA processing pattern indicative of co-regulated splicing of exons 2 and 3.</p> <p>Conclusions</p> <p>A combination of sensitive techniques may assure a high (100%) <it>STK11 </it>mutation detection frequency in PJS families. Characterization of mutations at mRNA level may give a deeper insight into the molecular consequences of the pathogenic mutations than predictions made solely at the genomic level.</p
BRCA/Fanconi anemia pathway genes in hereditary predisposition to breast cancer
Abstract
Two major genes are involved in hereditary predisposition to breast and ovarian cancer â BRCA1 and BRCA2. However, germline mutations in these tumor suppressors account for a maximum 20% of the familial breast cancer cases. A significant portion of the genes predisposing to this disease is unknown and therefore needs to be discovered. The aim of this study was to identify novel breast cancer susceptibility genes from the interweaving BRCA/Fanconi anemia (FA) pathway. Five candidate genes â MERIT40, ABRAXAS, BRIP1, CHK1, and FANCA â were screened for mutations by utilizing conformation-sensitive gel electrophoresis and sequencing, or with multiplex ligation-dependent probe amplification in blood DNA samples of Finnish familial breast cancer patients.
Investigation of the MERIT40 gene revealed novel nucleotide changes, being the first report on mutation screening of this gene. None of the observed alterations, however, appeared to be disease related, suggesting that germline mutations in MERIT40 are rare or absent in breast cancer patients.
A missense alteration (c.1082G>A, leading to Arg361Gln) was identified in ABRAXAS in 3 out of 125 Northern Finnish breast cancer families (2.4%), but not in any of the 867 healthy controls. The prevalence of the mutation between familial and control cases was statistically significantly different (p=0.002). ABRAXAS c.1082G>A appears to have pathological significance based on its exclusive occurrence in cancer cases, evolutionary conservation, disruption of a putative nuclear localization signal, reduced nuclear localization of the protein, and defective accumulation at DNA damage sites.
The BRIP1 (FANCJ) and CHK1 genes were screened for large genomic rearrangements, but no abnormalities were detected, ruling out a significant contribution to breast cancer susceptibility in the Northern Finnish population.
A novel large heterozygous deletion was identified in the FANCA gene in one out of 100 breast cancer families, removing the promoter and the first 12 exons. The deletion allele was not present in the tested controls, suggesting that it might contribute to breast cancer susceptibility. This is the first report on the association of a large-size germline deletion in a gene acting in the upstream part of the FA signaling pathway with familial breast cancer.TiivistelmÀ
BRCA1 ja BRCA2 ovat kaksi tĂ€rkeintĂ€ perinnöllisen rinta- ja munasarjasyövĂ€n alttiusgeeniĂ€. NiissĂ€ esiintyvĂ€t ituradan muutokset selittĂ€vĂ€t kuitenkin vain noin 20 % familiaalisista rintasyöpĂ€tapauksista. Suurin osa alttiusgeeneistĂ€ on edelleen tunnistamatta ja nĂ€itĂ€ tekijöitĂ€ etsitÀÀn aktiivisesti. TĂ€mĂ€n tutkimuksen tarkoituksena on ollut tunnistaa uusia alttiustekijöitĂ€ toisiinsa lĂ€heisesti liittyviltĂ€ BRCA/Fanconin anemia (FA) signaalinsiirtoreiteiltĂ€. Viisi kandidaattigeeniĂ€ - MERIT40, ABRAXAS, BRIP1, CHK1 ja FANCA â kartoitettiin mutaatioiden suhteen suomalaisissa rintasyöpĂ€perheissĂ€ kĂ€yttĂ€mĂ€llĂ€ konformaatiosensitiivistĂ€ geelielektroforeesia ja sekvensointia, tai multiplex ligation-dependent probe amplification- menetelmÀÀ.
MERIT40-geenissÀ havaittiin useita aikaisemmin raportoimattomia nukleotidimuutoksia, mutta yhdenkÀÀn niistÀ ei havaittu liittyvÀn rintasyöpÀalttiuteen. MERIT40-geenimuutosten mahdollista yhteyttÀ rintasyöpÀalttiuteen ei ole tutkittu aikaisemmin.
ABRAXAS-geenissÀ havaittiin missense-mutaatio (c.1082G>A, joka johtaa Arg361Gln aminohappokorvautumiseen) kolmessa pohjoissuomalaisessa rintasyöpÀperheessÀ (3/125, 2.4 %). Muutosta ei havaittu terveissÀ kontrolleissa (N=867), ja ero mutaation esiintyvyydessÀ familiaalisten rintasyöpÀtapausten ja terveiden kontrollien vÀlillÀ oli tilastollisesti merkitsevÀ (p=0.002). ABRAXAS c.1082G>A-muutos on todennÀköisesti patogeeninen, sillÀ kyseinen aminohappopaikka on evolutiivisesti konservoitunut ja sijaitsee todennÀköisellÀ tumaanohjaussignaalialueella. Funktionaaliset kokeet osoittivat, ettÀ mutatoitunut proteiinituote lokalisoitui villityypin proteiinia heikommin tumaan ja sen ohjautuminen DNA-vaurioalueille oli puutteellista.
BRIP1- (FANCJ) ja CHK1-geeneistÀ etsittiin laajoja genomisia uudelleenjÀrjestelyjÀ, mutta niitÀ ei havaittu. NÀin ollen kyseisillÀ muutoksilla ei ole merkittÀvÀÀ roolia perinnöllisessÀ rintasyöpÀalttiudessa suomalaisessa vÀestössÀ.
FANCA-geenissÀ havaittiin laaja heterotsygoottinen deleetio yhdessÀ tutkitusta 100 rintasyöpÀperheestÀ. Deleetio poistaa geenin promoottorialueen lisÀksi sen 12 ensimmÀistÀ eksonia. Deleetioalleelia ei havaittu terveissÀ kontrolleissa, joten se mahdollisesti liittyy perinnölliseen rintasyöpÀalttiuteen. Tutkimus on ensimmÀinen, jossa raportoidaan laaja genominen deleetio FA-signaalinsiirtoreitin ylÀvirran geenissÀ familiaalisessa rintasyövÀssÀ
DNA-Binding Deficiency of Trp690 and Phe733 Mutants
<div><p>(A) Pull-down assays were performed by coincubating <i>Sf</i>9 cell lysate (5 Όl) containing wild-type XPC or the indicated Ala mutants and single-stranded DNA beads. The binding buffer contained 0.3 M NaCl. The fractions of free (F) and bound (B) protein were separated and analyzed by gel electrophoresis and immunobloting using specific monoclonal antibodies. The panel on the right shows the quantitative evaluation of three independent binding assays (± SD).</p>
<p>(B) Side-by-side comparison of the DNA-binding capacity of wild-type XPC protein (lanes 7 and 8) and the indicated mutants (lanes 1â6).</p></div
The Trp690Ser Mutant Is Defective in Substrate Binding
<div><p>(A) Pull-down assays were performed by coincubating <i>Sf</i>9 cell lysate (5 ÎŒl) containing wild-type XPC protein and 50 ÎŒl of single-stranded DNA beads. The binding buffer was supplemented with the indicated concentrations of NaCl. The fractions of free (F) and bound (B) protein were separated and analyzed by gel electrophoresis and immunobloting using specific monoclonal antibodies. The panel on the right provides a quantitative evaluation of three independent binding assays showing the proportion of pulled-down XPC protein at the different ionic strengths.</p>
<p>(B) Pull-down assay with <i>Sf</i>9 cell lysate (5 ÎŒl) containing the Trp690Ser mutant (left) and quantitative evaluation of three independent experiments (right).</p></div
Screening for Repair-Deficient XPC Mutants
<div><p>(A) Time course of host cell reactivation assay. Human XPâC fibroblasts were transfected with an expression vector coding for wild-type XPC (pcXPC) or the empty control vector (pcDNA). Repair complementation was assessed after the indicated times by monitoring luciferase expression from a UV-irradiated reporter construct. Excision is reported as the percentage of wild-type activity after 15 h of incubation (± SD).</p>
<p>(B) Specificity of the repair assay. XPâC fibroblasts were transfected with expression vectors coding for wild-type XPC (pcXPC or pXPCâGFP), wild-type XPA (pcXPA), the control vectors (pcDNA and pGFP), or vectors containing the Trp690Ser mutant sequences (pcW690S or pW690SâGFP). Excision is reported as the percentage of wild-type activity (15-h incubations).</p>
<p>(C) Deletion of aromatic side chains. XPâC fibroblasts were transfected with vector pcXPC carrying the indicated mutations. DNA repair is expressed as the percentage of wild-type complementation (15-h incubations) after deduction of background luciferase activity obtained with the control vector. The dashed line indicates a threshold of 50% reduction in repair activity.</p>
<p>(D) Replacement of aromatic residues by amino acids of different properties.</p>
<p>(E) Deletion of the positively charged side chains of conserved Lys or Arg residues.</p>
<p>(F) Ala substitutions of absolutely conserved positions in the center of the DNA-binding domain.</p></div