12 research outputs found
Clinical and molecular markers in retinal detachment—From hyperreflective points to stem cells and inflammation
Clinical and molecular markers in retinal detachment: From hyperreflective points to stem cells and inflammation
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Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium-Role in Dead Cell Clearance and Inflammation
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Thermogenic Activation Downregulates High Mitophagy Rate in Human Masked and Mature Beige Adipocytes
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Clearance of autophagy-associated dying retinal pigment epithelial cells - a possible source for inflammation in age-related macular degeneration
Cell death, clearance and inflammation: molecular crossroads and gene polymorphisms in the pathogenesis of age-related macular degeneration
Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium-Role in Dead Cell Clearance and Inflammation
Mitophagy Mediates the Beige to White Transition of Human Primary Subcutaneous Adipocytes Ex Vivo
Brown and beige adipocytes have multilocular lipid droplets, express uncoupling protein (UCP) 1, and promote energy expenditure. In rodents, when the stimulus of browning subsides, parkin-dependent mitophagy is activated and dormant beige adipocytes persist. In humans, however, the molecular events during the beige to white transition have not been studied in detail. In this study, human primary subcutaneous abdominal preadipocytes were differentiated to beige for 14 days, then either the beige culture conditions were applied for an additional 14 days or it was replaced by a white medium. Control white adipocytes were differentiated by their specific cocktail for 28 days. Peroxisome proliferator-activated receptor γ-driven beige differentiation resulted in increased mitochondrial biogenesis, UCP1 expression, fragmentation, and respiration as compared to white. Morphology, UCP1 content, mitochondrial fragmentation, and basal respiration of the adipocytes that underwent transition, along with the induction of mitophagy, were similar to control white adipocytes. However, white converted beige adipocytes had a stronger responsiveness to dibutyril-cAMP, which mimics adrenergic stimulus, than the control white ones. Gene expression patterns showed that the removal of mitochondria in transitioning adipocytes may involve both parkin-dependent and -independent pathways. Preventing the entry of beige adipocytes into white transition can be a feasible way to maintain elevated thermogenesis and energy expenditure
Triamcinolone regulated apopto-phagocytic gene expression patterns in the clearance of dying retinal pigment epithelial cells. A key role of Mertk in the enhanced phagocytosis
AbstractBackground The apopto-phagocytic gene expression
patterns during clearance of dying cells in the retina and the
effect of triamcinolone (TC) upon these processes have
relevance to development of age-related macular degeneration
(AMD). Methods ARPE-19 cells and primary human retinal pigment
epithelium (hRPE) were induced to undergo cell death by
anoikis and the clearance of these cells by living hRPE/ARPE-
19 or human monocyte-derived macrophages (HMDMs) in the
presence or absence of TC was quantified by flow cytometry.
TaqMan low-density gene expression array determining known
markers of phagocytosis and loss-of-function studies on
selected apopto-phagocytic genes was carried out in HMDM
engulfing anoikic cells. Results The glucocorticoid TC had a
profound phagocytosis-enhancing effect on HMDM engulfing
anoikic ARPE-19 or hRPE cells, causing a selective
upregulation of the Mer tyrosine kinase (MERTK) receptor,
while decreasing the expression of the AXL receptor tyrosine
kinase and thrombospondin-1 (THSB-1). The key role of the
MERTK could be demonstrated in HMDM engulfing dying cells
using gene silencing as well as blocking antibodies. Similar
pathways were found upregulated in living ARPE-19 engulfing
anoikic ARPE-19 cells. Gas6 treatment enhanced phagocytosis in
TC-treated HMDMs. Conclusions Specific agonists of the Mertk
receptor may have a potential role as phagocytosis enhancers
in the retina and serve as future targets for AMD therapy.
General significance The use of Gas6 as enhancer of retinal
phagocytosis via the MerTK receptor, alone or in combination
with other specific ligands of the tyrosine kinase receptors'
family may have a potential role in AMD therapy