The discursive construction of low-risk to sexually transmitted diseases between women who are sexually active with women

This paper uses discursive analytical method to explore dominant discourses concerning the sexual health of women who have sex with women. In-depth interviews were conducted with a cross-cultural sample of women from England and Brazil. Sex between women was discursively constructed as ‘safe’ and women who have sex with women were seen as being at low to negligible risk of contracting/transmitting sexually transmitted infections. Analysis identified two discourses underlying these constructions: a binaries discourse which focused on dichotomies of gender, sexuality and risk; and a sexual double-standard discourse, which focused on the positioning of sex between women as safe and the use of barrier methods of protection as indicative of not engaging or fully enjoying the sexual act

A qualitative study to understand patients\u27 experiences of foot pain and surgical consultation for hallux valgus and/or hallux rigidus

The foot is one of the most complex musculoskeletal systems in the body, playing a pivotal role in gait and posture. Epidemiological studies indicate that a high prevalence of foot problems is associated with a greater burden of pain, disability and decreased health-related quality of life. Disabling foot pain is likely to be multifactorial in origin, however to date there is limited evidence of the psychosocial impact of forefoot problems, as such the associated burden is unknown. The study was qualitative with the use of semi-structured interviews to explore patients\u27 experiences of their foot problem and of undergoing surgical consultation and/or intervention for hallux valgus and/or hallux rigidus. Sixteen patients participated: 14 females and 2 males; mean age of 61 years, SD 7.23 from a National Health Service secondary care hospital within an orthopaedic department, who required surgery for hallux valgus and/or hallux rigidus. Thematic analysis generated three themes; \u27the impact of pain\u27, \u27the decision-making process\u27 and \u27body image, the self and identity\u27. Forefoot problems present many issues for patients and impact on their physical, social and psychological health. Forefoot problems are multifactorial and patients should be supported using a biopsychosocial approach

Recon 2.2: from reconstruction to model of human metabolism.

IntroductionThe human genome-scale metabolic reconstruction details all known metabolic reactions occurring in humans, and thereby holds substantial promise for studying complex diseases and phenotypes. Capturing the whole human metabolic reconstruction is an on-going task and since the last community effort generated a consensus reconstruction, several updates have been developed.ObjectivesWe report a new consensus version, Recon 2.2, which integrates various alternative versions with significant additional updates. In addition to re-establishing a consensus reconstruction, further key objectives included providing more comprehensive annotation of metabolites and genes, ensuring full mass and charge balance in all reactions, and developing a model that correctly predicts ATP production on a range of carbon sources.MethodsRecon 2.2 has been developed through a combination of manual curation and automated error checking. Specific and significant manual updates include a respecification of fatty acid metabolism, oxidative phosphorylation and a coupling of the electron transport chain to ATP synthase activity. All metabolites have definitive chemical formulae and charges specified, and these are used to ensure full mass and charge reaction balancing through an automated linear programming approach. Additionally, improved integration with transcriptomics and proteomics data has been facilitated with the updated curation of relationships between genes, proteins and reactions.ResultsRecon 2.2 now represents the most predictive model of human metabolism to date as demonstrated here. Extensive manual curation has increased the reconstruction size to 5324 metabolites, 7785 reactions and 1675 associated genes, which now are mapped to a single standard. The focus upon mass and charge balancing of all reactions, along with better representation of energy generation, has produced a flux model that correctly predicts ATP yield on different carbon sources.ConclusionThrough these updates we have achieved the most complete and best annotated consensus human metabolic reconstruction available, thereby increasing the ability of this resource to provide novel insights into normal and disease states in human. The model is freely available from the Biomodels database (http://identifiers.org/biomodels.db/MODEL1603150001)