Hydroxyproline determination in serum and gingival crevicular fluid

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65681/1/j.1600-0765.1987.tb01552.x.pd

Hydroxyproline titers in gingival crevicular fluid

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65217/1/j.1600-0765.1987.tb01570.x.pd

Differences between gingivitis and periodontitis associated microbial flora in the beagle dog

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65244/1/j.1600-0765.1982.tb01126.x.pd

Design of acoustic metamaterials through nonlinear programming

The dispersive wave propagation in a periodic metamaterial with tetrachiral topology and inertial local resonators is investigated. The Floquet-Bloch spectrum of the metamaterial is compared with that of the tetrachiral beam lattice material without resonators. The resonators can be designed to open and shift frequency band gaps, that is, spectrum intervals in which harmonic waves do not propagate. Therefore, an optimal passive control of the frequency band structure can be pursued in the metamaterial. To this aim, a suitable constrained nonlinear optimization problem on a compact set of admissible geometrical and mechanical parameters is stated. According to functional requirements, the particular set of parameters which determines the largest low-frequency band gap between a pair of consecutive branches of the Floquet-Bloch spectrum is obtained. The optimization problem is successfully solved by means of a version of the method of moving asymptotes, combined with a quasi-Monte Carlo multi-start technique. Subjects: Materials Science (cond-mat.mtrl-sci) Cite as: arXiv:1603.07717 [cond-mat.mtrl-sci] (or arXiv:1603.07717v2 [cond-mat.mtrl-sci] for this version

Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice.

The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated

Laser-induced fluorescence in malignant and normal tissue in mice injected with two different carotenoporphyrins.

Laser-induced fluorescence (LIF) was used to characterise the localisation of an intravenously administered trimethylated carotenoporphyrin [CP(Me)3] and a trimethoxylated carotenoporphyrin [CP(OMe)3] in an intramuscularly transplanted malignant tumour (MS-2 fibrosarcoma) and healthy muscle in female Balb/c mice, 3, 24, 48 and 96 h post injection. The fluorescence was induced with a dye laser pumped by a nitrogen laser, emitting light at 425 nm. The fluorescence spectra were recorded in the region 455-760 nm using a polychromator equipped with an image-intensified CCD camera. The tumour/peritumoral muscle ratio was about 5:1 for CP(Me)3 and about 6:1 for CP(OMe)3 in terms of the background-free fluorescence intensity, which peaked at about 655 nm. By including the endogenous tissue fluorescence, the contrast was further enhanced by a factor of approximately 2

Radiative properties of EuII and EuIII obtained from lifetime and branching ratio measurements

Radiative lifetimes of nine levels in Eu II (4f(7)6p, 4f(6)5d6s and 4f(6)5d(2)) and Eu III (4f(6)5d) have been measured using time-resolved laser-induced fluorescence spectroscopy on a laser-produced europium plasma. Oscillator strengths for a number of Eu II lines have been derived by combining the lifetimes with branching ratios measured in the emission spectrum of a hollow-cathode lamp

Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin

<p>Abstract</p> <p>Background</p> <p>Camptothecin is a plant alkaloid that specifically binds topoisomerase I, inhibiting its activity and inducing double stranded breaks in DNA, activating the cell responses to DNA damage and, in response to severe treatments, triggering cell death.</p> <p>Results</p> <p>Comparative transcriptomic and proteomic analyses of maize embryos that had been exposed to camptothecin were conducted. Under the conditions used in this study, camptothecin did not induce extensive degradation in the genomic DNA but induced the transcription of genes involved in DNA repair and repressed genes involved in cell division. Camptothecin also affected the accumulation of several proteins involved in the stress response and induced the activity of certain calcium-dependent nucleases. We also detected changes in the expression and accumulation of different genes and proteins involved in post-translational regulatory processes.</p> <p>Conclusions</p> <p>This study identified several genes and proteins that participate in DNA damage responses in plants. Some of them may be involved in general responses to stress, but others are candidate genes for specific involvement in DNA repair. Our results open a number of new avenues for researching and improving plant resistance to DNA injury.</p