Song Gede: Situs Gua Hunian Sejak Masa Pleistosen Akhir di Pulau Nusa Penida, Bali: Song Gede Site: Cave Dwelling Site Since Late Pleistocene Period in the Nusa Penida Island, Bali

Prehistoric archaeological research in Indonesia recently showed the existence of dwelling caves from the late Pleistocene, starting from Sumatra, Java, Sulawesi to East Nusa Tenggara. The Song Gede site is one of the dwelling caves from the late Pleistocene which was very interesting to be discused, especially when tracing the distribution of early modern humans in Southeast Asia. The location of the Song Gede site is considered to have a very strategic position because it was located at the eastern end of the Sunda Shelf towards Wallacea. The purpose of this paper is to study the chronology of the Song Gede Site and review the results of research at the Song Gede Site as an ocupation site that is on the migration route in the southern part of the Sunda Shelf to the Wallacea region or vice versa. The research method used was stratigraphic analysis, contextual and carbon dating methods. The results showed that the Song Gede Site has been inhabited since the late Pleistocene to the Holocene. The human living in the cave uses various natural resources to meet their daily needs, such as the use of rock materials, and the rest of the animal remains for tools and the use of animal and plants to fulfill their daily consumption

Locomotion in Homo floresiensis: reconstructing foot use from the internal bone structure of the metatarsals of LB1

The enigmatic Homo floresiensis displays a unique combination of cranial and post-cranial morphology [1-3], distinguishing it from other species of the genus Homo. Although its skeletal anatomy shows clear adaptations for terrestrial bipedalism, it also retains a suite of features conducive to arboreal behaviours. Thus, exactly how the locomotor behaviours of H. floresiensis compare with those of other hominins remains an important research question. The foot of the holotype (LB1) is long relative to its femoral length, has a longer forefoot than hindfoot, long phalanges relative to the non-hallucial metatarsals (Mts), and a short Mt1 relative to the other Mts [3]. However, it also possesses a human-like Mt head morphology and relative robusticity pattern [2-3]. Here, we assess the internal morphology of the Mts of LB1 to further assess foot functional morphology and locomotor kinematics in H. floresiensis. Using high resolution micro-CT scans of the Mts of LB1 and a comparative sample of Homo sapiens (N=10), Pan troglodytes (N=15), Pan paniscus (N=15), Gorilla spp. (N=10) and Pongo spp. (N=9), we conducted a cross-sectional geometric analysis at mid-shaft and analysis of trabecular bone distribution in the Mt head. As the head was only fully preserved for the right Mt5 of LB1, trabecular analysis was limited to this element.Cross-sectional geometry of the Mts at mid-shaft distinguishes between ape-like and human-like biomechanics, with greater loading of the Mt2 and Mt3 in apes compared with more lateral loading in humans [4]. The Mts of LB1 are internally robust, having a high cross-sectional area relative to bone length. Results show that the relative strength of the Mts, based on the internal structure, differs from the previously reported human-like pattern, which was based on external measurements of midshaft circumference [2-3]. We find that, after scaling by total bone length, the robusticity pattern for the left Mts of LB1 is 1>2>5>3>4 for CSA and Z, and 1>5>2>4>3 for J. Although there is some variation among humans, in general the Mt3 and Mt2 have lower measures of robusticity than the Mt4 and Mt5 [4]. In LB1, the Mt2 is consistently more robust than is expected in humans, with the pattern being 1>2/5>3/4 compared to 1>4/5>2/3 in humans. The distribution of trabecular bone in the Mt5 head distinguishes between locomotor groups. In H. sapiens, where the foot is loaded in dorsiflexion there is a dorsal concentration of bone, which is asymmetric in extending dorsomedially. In African apes, where the toes are positioned dorsally during knuckle-walking and disto-plantarly during climbing (depending on substrate size) the distribution of trabecular bone extends dorsally to plantarly on the metatarsal head. In Pongo, trabecular bone is distributed distally and plantarly reflecting a grasping foot. The distribution of trabecular bone in the Mt5 of LB1 is located dorsally and distally but does not extend plantarly. This distribution pattern differs from humans in being centrally located, rather than medially, and in extending further distally. This suggests that the metatarsophalangeal joint in LB1 was loaded in a more a neutral position than in humans.Together, the results suggest that loading of the foot of H. floresiensis differed from modern humans. First, the distribution of load across the foot was likely higher in the Mt2, a feature that could relate to higher loading of the second ray in a foot with a relatively short first ray. Secondly, the trabecular pattern suggests loading of the Mt5 head more distally than in humans, and with less asymmetric loading. This differing position of the metatarsophalangeal joint could be related to the long, curved phalanges of LB1. Future research exploring whole-bone cortical and trabecular structure of the metatarsals will shed new light on the kinematics of locomotion in H. floresiensis

Temporal shifts in the distribution of murine rodent body size classes at Liang Bua (Flores, Indonesia) reveal new insights into the paleoecology of Homo floresiensis and associated fauna

Liang Bua, the type locality of Homo floresiensis, is a limestone cave located in the western part of the Indonesian island of Flores. The relatively continuous stratigraphic sequence of the site spans the past ∼190 kyr and contains ∼275,000 taxonomically identifiable vertebrate skeletal elements, ∼80% of which belong to murine rodent taxa (i.e., rats). Six described genera are present at Liang Bua (Papagomys, Spelaeomys, Hooijeromys, Komodomys, Paulamys, and Rattus), one of which, Hooijeromys, is newly recorded in the site deposits, being previously known only from Early to Middle Pleistocene sites in central Flores. Measurements of the proximal femur (n = 10,212) and distal humerus (n = 1186) indicate five murine body size classes ranging from small (mouse-sized) to giant (common rabbit-sized) are present. The proportions of these five classes across successive stratigraphic units reveal two major changes in murine body size distribution due to significant shifts in the abundances of more open habitat-adapted medium-sized murines versus more closed habitat-adapted smaller-sized ones. One of these changes suggests a modest increase in available open habitats occurred ∼3 ka, likely the result of anthropogenic changes to the landscape related to farming by modern human populations. The other and more significant change occurred ∼60 ka suggesting a rapid shift from more open habitats to more closed conditions at this time. The abrupt reduction of medium-sized murines, along with the disappearance of H. floresiensis, Stegodon florensis insularis (an extinct proboscidean), Varanus komodoensis (Komodo dragon), Leptoptilos robustus (giant marabou stork), and Trigonoceps sp. (vulture) at Liang Bua ∼60-50 ka, is likely the consequence of these animals preferring and tracking more open habitats to elsewhere on the island. If correct, then the precise timing and nature of the extinction of H. floresiensis and its contemporaries must await new discoveries at Liang Bua or other as yet unexcavated sites on Flores

Development and application of a comprehensive analytical workflow for the quantification of non-volatile low molecular weight lipids on archaeological stone tools

Source determination of use-related residues on prehistoric stone tools is especially challenging, due to issues related to preservation, contamination and the contribution of residues from multiple sources. To increase confidence in this process, an analytical workflow was developed to include: (1) a sampling strategy that retains spatial information of the recovered residues and enables monitoring of environmental contamination; and (2) a sensitive and selective gas chromatography-tandem mass spectrometry (GC-MS/MS) procedure to quantify non-volatile low molecular weight lipids on stone artefacts. This workflow was applied to 14 stone artefacts excavated from deposits at Liang Bua, a limestone cave on the Indonesian island of Flores. These artefacts range in age between ∼14 000 and 1000 years old, and were preliminarily classified as either potentially showing traces of use (n = 7) or not (n = 7) using low magnification microscopy. Residues were sampled by direct solvent extraction off the surface of the artefacts. The aliquots were spiked with internal standards and derivatised. The trimethylsilyl derivatives of 40 saturated fatty acids, sterols, di- and tri-terpenoids and their analogues were quantified using optimised multiple reaction monitoring (MRM) transitions. Six of the potentially used artefacts contained sterols, phytosterols and terpenoids, either individually or in combination, whereas none of these compounds was commonly found on the seven artefacts preliminarily classified as unused. This suggests that these six artefacts may have been used as implements to process resources, and provides scope for further investigation. This workflow can also be adapted for the analysis of other archaeological objects

Combined organic biomarker and use-wear analyses of stone artefacts from Liang Bua, Flores, Indonesia

Organic biomarker and lithic use-wear analyses of archaeological implements manufactured and/or used by hominins in the past offers a means of assessing how prehistoric peoples utilised natural resources. Currently, most studies focus on one of these techniques, rather than using both in sequence. This study aims to assess the potential of combining both methods to analyse stone artefacts, using a set of 69 stones excavated from the cave site of Liang Bua (Flores, Indonesia). Prior to chemical analysis, an initial inspection of the artefacts revealed potential use-wear traces but no visible residues. Gas chromatography mass spectrometry (GC-MS) analysis, including the targeting of 86 lipids, terpenes, terpenoids, alkanes and their analogues, found compounds with plant or animal origin on 27 of the 69 stones. The artefacts were subsequently cleaned, and use-wear analysis identified traces of use on 43 artefacts. Use-wear analysis confirmed traces of use on 23 of the 27 artefacts with potential use-residues that were determined by GC-MS. The GC-MS results were broadly consistent with the functional classes identified in the later use-wear analysis. This inclusive approach for stone artefact analysis strengthens the identifications made through multiple lines of enquiry. There remain conflicts and uncertainties in specific cases, suggesting the need for further refinement and analyses of the relationships between use-wear and residues

Age and context of the oldest known hominin fossils from Flores

Recent excavations at the early Middle Pleistocene site of Mata Menge in the So\u27a Basin of central Flores, Indonesia, have yielded hominin fossils1 attributed to a population ancestral to Late Pleistocene Homo floresiensis2. Here we describe the age and context of the Mata Menge hominin specimens and associated archaeological findings. The fluvial sandstone layer from which the in situ fossils were excavated in 2014 was deposited in a small valley stream around 700 thousand years ago, as indicated by 40Ar/39Ar and fission track dates on stratigraphically bracketing volcanic ash and pyroclastic density current deposits, in combination with coupled uranium-series and electron spin resonance dating of fossil teeth. Palaeoenvironmental data indicate a relatively dry climate in the So\u27a Basin during the early Middle Pleistocene, while various lines of evidence suggest the hominins inhabited a savannah-like open grassland habitat with a wetland component. The hominin fossils occur alongside the remains of an insular fauna and a simple stone technology that is markedly similar to that associated with Late Pleistocene H. floresiensis

An integrative geochronological framework for the pleistocene So'a basin (Flores, Indonesia), and its implications for faunal turnover and hominin arrival

Flores represents a unique insular environment with an extensive record of Pleistocene fossil remains and stone artefacts. In the So\u27a Basin of central Flores these include endemic Stegodon, Komodo dragons, giant tortoises, rats, birds and hominins, and lithic artefacts that can be traced back to at least one million years ago (1 Ma). This comprehensive review presents important new data regarding the dating and faunal sequence of the So\u27a Basin, including the site of Mata Menge where Homo floresiensis-like fossils dating to approximately 0.7 Ma were discovered in 2014. By chemical fingerprinting key silicic tephra originating from local and distal eruptive sources we have now established basin-wide tephrostratigraphic correlations, and, together with new numerical ages, present an update of the chronostratigraphy of the So\u27a Basin, with major implications for the faunal sequence. These results show that a giant tortoise and the diminutive proboscidean Stegodon sondaari last occurred at the site of Tangi Talo ∼1.3 Ma, and not 0.9 Ma as previously thought. We also present new data suggesting that the disappearance of giant tortoise and S. sondaari from the sedimentary record occurred before, and/or was coincident with, the earliest hominin arrival, as evidenced by the first records of lithic artefacts occurring directly below the 1 Ma Wolo Sege Tephra. Artefacts become common in the younger layers, associated with a distinct fauna characterized by the medium-sized Stegodon florensis and giant rat Hooijeromys nusatenggara. Furthermore, we describe a newly discovered terrace fill, which extends the faunal record of Stegodon in the So\u27a Basin to the Late Pleistocene. Our evidence also suggests that the paleoenvironment of the So\u27a Basin became drier around the time of the observed faunal transition and arrival of hominins on the island, which could be related to an astronomically-forced climate response at the onset of the Mid-Pleistocene Transition (MPT; ∼1.25 Ma) leading to increased aridity and monsoonal intensity

New archaeological research at Liang Bua on the island of Flores: implications for the extinction of Homo floresiensis and the arrival of Homo sapiens in eastern Indonesia

The discovery in 2003 of the partial skeleton of a diminutive and primitive hominin species, Homo floresiensis, has generated wide interest and scientific debate. These remains were found buried in cave sediments at the site of Liang Bua on the island of Flores, eastern Indonesia, in association with stone artefacts and the remains of other extinct endemic fauna, such as pygmy Stegodon, marabou stork and vulture. A major reason that H. floresiensis has stirred such controversy is because the associated deposits were dated to between about 95 and 12 thousand calendar years (ka) ago. These ages implied that H. floresiensis survived on Flores long after modern humans (H. sapiens) had arrived in Australia by about 50 ka ago, thus posing a challenge to prevailing notions of modern human dispersals and human evolution in Southeast Asia and Australasia. The research conducted for this thesis is aimed at addressing several unresolved questions arising from the original investigations at Liang Bua. A series of new excavations were conducted at Liang Bua between 2007 and 2014 to shed further light on the history of site formation and the hominin skeletal and cultural remains preserved within the cave deposits. The stratigraphic, chronological, archaeological and faunal evidence gleaned from these excavations is described and analysed in this thesis, and this information is integrated with that obtained in 2001–2004 to culminate in a new interpretation of the time of H. floresiensis extinction and H. sapiens arrival at Liang Bua