Comparative metallomics for transgenic and non-transgenic soybeans

In this work, a comparative metallomics of transgenic and non- transgenic soybeans [ Glycine max ( L.) Merrill] was performed. Soybean proteins were extracted with a proper buffer and separated by two- dimensional polyacrylamide gel electrophoresis. Metal ions bound to a set of eight proteins randomly selected ( ranging from 13.98 to 54.87 kDa), were characterized by matrixassisted laser desorption- ionization quadrupole- time of flight mass spectrometry and mapped using synchrotron radiation X- ray spectrometry. The metal ions detected were: Ca( II), Cu( II), Fe( II), Mn( II), Ni( II) and Zn( II). Transgenic and non- transgenic soybeans proteins were found to display typical and random profiles for metal ions binding. To test the reliability of the qualitative metal ions profiles, quantification of Ca( II), Cu( II) and Fe( II) was performed via microwave-assisted decomposition in mini- vials followed by atomic absorption spectrometry determination. Qualitative and quantitative metallomics was found to be coherent and to match profiles expected from the known protein functions. The protein of spot 5, with molar mass of 37.62 kDa ( amino acid sequence presented), was found to display the most characteristic change in metal ions content, with higher Ca( II), Cu( II) and Fe( II) concentrations for transgenic soybeans.22121501150

Combination of PAGE and LA-ICP-MS as an analytical workflow in metallomics: state of the art, new quantification strategies, advantages ans limitations

Metallomics (more specifically, metalloproteomics) is an emerging field that encompasses the role, uptake, transport and storage of trace metals, which are essential to preserve the functions of proteins within a biological system. The current strategies for metal-binding and metalloprotein analysis based on the combination of polyacrylamide gel electrophoresis (PAGE) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) are discussed in this review. The advantages, limitations and the most recently developed and applied quantification approaches for this methodology are also described

Determination Of Chromium (vi) By Flame Atomic Absorption Spectrometry After Cloud Point Extraction And Preconcentration [determinação De Cromo (vi) Por Espectrometria De Absorção Atômica Com Chama Após A Extraçã O E Pré-concentração No Ponto Nuvem]

The present work reports a method for chromium (VI) determination by flame atomic absorption spectrometry (FAAS) after cloud point extraction and preconcentration. Chromium (VI) is complexed with 1,5-diphenylcarbazide (DFC) in acidic medium (pH 2.0) and it is extracted into 25 μL of surfactant-rich phase containing Triton X-114. The variables that affect the cloud point formation, such as the surfactant (0.1-1.0% m/v) and complexant (0.01-0.80% m/v) concentrations, time of complexation (0-60 min), and effect of electrolyte NaCl (0-20% m/v) addition are evaluated. Under optimized conditions, 0.3% m/v Triton X-114, 0.05% m/v DFC and 10% m/v NaCl are used for chromium (VI) extraction. This method allows detection and quantification limits of 0.4 μg L -1 and 1.5 μg L-1, respectively, and a linear calibration range from 5 to 500 μg L-1. The preconcentration factor obtained is 27 and the extraction efficiency achieved varies from 87 to 99.3%.3117380Manzoori, J.L., Sorouraddin, M.H., Shemiran, F., (1996) Anal. Lett., 29 (11), p. 2007Wang, C., Martin, D.F., Martin, B.B., (1999) J. Environ. Sci. Health, A34 (3), p. 705Padarauskas, A., Judzentiene, A., Naujalis, E., Paliulionyte, V., (1998) J. Chromatogr. A, 808 (1-2), p. 193Shemirani, F., Abkenar, S.D., Mirroshandel, A.A., Niasari, M.S., Kozania, R.R., (2003) Anal. Sci., 19 (10), p. 1453Paleologos, E.K., Vlessidis, A.G., Karayannis, M.I., Evmiridis, N.P., (2003) Anal. Chim. Acta, 477 (2), p. 223Paleologos, E.K., Stalikas, C.D., Tzouwara-Karayanni, S.M., Karayannis, M.I., (2001) Anal. Chim. Acta, 436 (1), p. 49Giokas, D.L., Paleologos, E.K., Tzouwara-Karayanni, S.M., Karayannis, M.I., (2001) J. Anal. At. Spectrom., 16 (5), p. 521Nascentes, C.C., Arruda, M.A.Z., (2003) Talanta, 61, p. 759Paleologos, E.K., Stalikas, C.D., Tzouwara-Karayanni, S.M., Pilidis, G.A., Karayannis, M.I., (2000) J. Anal. At. Spectrom., 15 (3), p. 287Coelho, L.M., Arruda, M.A.Z., (2005) Spectrochim. Acta B, 60 (5), p. 743Cordero, B.M., Pavón, J.L.P., Pinto, C.G., Laespada, M.E.F., (1993) Talanta, 40 (11), p. 1703Maniasso, N., (2001) Quim. Nova, 24 (1), p. 87Hinze, W.L., Pramauro, E., (1993) Crit. Rev. Anal. Chem., 24 (2), p. 133Stalikas, C.D., (2002) Trends Anal. Chem., 21 (5), p. 343Bezerra, M.A., Arruda, M.A.Z., Ferreira, S.L.C., (2005) Appl. Spectrosc. Rev., 40 (4), p. 269Sanz-Medel, A., De La Campa, M.R.F., Gonzalez, E.B., Fernandez-Sanchez, M.L., (1999) Spectrochim. Acta B, 54 (2), p. 251Willems, G.J., Blaton, N.M., Peeters, O.M., De Ranter, C.J., (1977) Anal. Chim. Acta, 88 (2), p. 345Nascentes, C.C., (2002) Emprego de Diferentes Estratégias para Análises Em Larga Escala: Screening, Extração Ultra-sônica e Pré-concentração Por Ponto Nuvem, p. 140. , Tese de Doutorado, Unicamp, CampinasKomaromy-Hiller, G., Calkins, N., Wandruszka, R., (1996) Langmuir, 12, p. 916Tarley, C.R.T., Arruda, M.A.Z., (2004) Anal. Sci., 20, p. 961Ferreira, S.L.C., Lemos, V.A., Moreira, B.C., Costa, A.C.S., Santelli, R.E., (2000) Anal. Chim. Acta, 403, p. 259(1987) Analyst, 11, p. 119http://www.mma.gov.br/port/conama/res/res86/res2086.htm

Determination of chromium (VI) by flame atomic absorption spectrometry after cloud point extraction and preconcentration

The present work reports a method for chromium (VI) determination by flame atomic absorption spectrometry (FAAS) after cloud point extraction and preconcentration. Chromium (VI) is complexed with 1,5-diphenylcarbazide (DFC) in acidic medium (pH 2.0) and it is extracted into 25 µL of surfactant-rich phase containing Triton X-114. The variables that affect the cloud point formation, such as the surfactant (0.1-1.0% m/v) and complexant (0.01-0.80% m/v) concentrations, time of complexation (0-60 min), and effect of electrolyte NaCl (0-20% m/v) addition are evaluated. Under optimized conditions, 0.3% m/v Triton X-114, 0.05% m/v DFC and 10% m/v NaCl are used for chromium (VI) extraction. This method allows detection and quantification limits of 0.4 µg L-1 and 1.5 µg L-1, respectively, and a linear calibration range from 5 to 500 µg L-1. The preconcentration factor obtained is 27 and the extraction efficiency achieved varies from 87 to 99.3%.O presente trabalho reporta um método para a determinação de cromo (VI) por espectrometria de absorção atômica com chama (FAAS) após a extração e pré-concentração no ponto nuvem. O cromo (VI) é complexado com 1,5-difenilcarbazida (DFC) em meio ácido (pH 2,0) e é extraído para um volume de fase rica de 25 µL, com o uso do surfactante Triton X-114. As variáveis que afetam a formação do ponto nuvem, tais como a concentração de surfactante (0,1-1,0% m/v) e de complexante (0,01-0,80% m/v), o tempo de complexação (0-60 min) e o efeito da adição do eletrólito NaCl (0-20% m/v), são avaliadas. Sob condições otimizadas, são utilizados o Triton X-114 a 0,3% m/v, a DFC a 0,05% m/v e o NaCl a 10% m/v para a extração do cromo (VI). Esse método fornece limites de detecção e quantificação de 0,4 µg L-1 e 1,5 µg L-1, respectivamente, e uma faixa linear de calibração de 5 a 500 µg L-1. O fator de pré-concentração obtido é igual a 27 e a eficiência de extração varia de 87 a 99,3%.7380Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Exploratory analysis of the serum ionomic profile for bipolar disorder and lithium treatment

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)In the present work, a very simple and fast experimental design using ICP-MS was employed to search for differentiations in the levels of free ions present in human blood serum samples obtained from healthy individuals (n = 25) and bipolar disorder (BD) patients under different treatments: (i) using lithium (n = 15) and (ii) using other drugs than lithium (n = 10). Fourteen ions that showed statistically significant alterations in its levels when comparing the studied groups were detected, providing a hint for further studies on BD and lithium treatment biomarker discovery. (C) 2010 Elsevier B.V. All rights reserved.30741699SI182184Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Financiadora de Estudos e Projetos (FINEP, Brasilia, Brazil)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPESP [06/58073-0

Microwave-assisted Decomposition Of Polyacrylamide Gels Containing Metalloproteins Using Mini-vials: An Auxiliary Strategy For Metallomics Studies

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