Ultrasonication mode for the expedition of extraction process of chitin from the maritime shrimp shell waste

431-438Worldwide, marine crustacean waste is a major problem for environmental pollution, and it is a severe risk to the coastline area. Shellfish wastes consist of some commercially valuable products, mainly the chitin. The extraction of chitin from the shellfish waste is very complicated and required a successive pretreatment process. Sonication can improve the process of extraction of chitin from the shrimp shell waste. In this study, the conventional and ultrasonication method of pretreatment was applied and compared for the extraction of chitin. By the conventional method, 12 h was required for the removal of calcium and proteins each. In contrast, only 6 h was required for the complete removal of calcium and proteins each, by the ultrasonication assisted method. After pretreatment, the results were analyzed and compared by the already purified commercial chitin using Fourier-transform infrared spectroscopy. Ultrasonication improves the rate of reaction of the pretreatment by the process of cavitation. By this work, the ultrasonication technique was proved to be much faster than the conventional method for the pretreatment process

Computer Based Screening of Selected Phytoconstituents from Cyperus Rotundus Linn. Against 5 α Reductase Enzyme

Hirsutism is a condition of unwanted, male-pattern terminal hair growth in women. Electro epilation, laser treatment, intense pulsed light therapy, eflornithine cream, and oral antiandrogen medications are the various allopathic therapeutics have been employed for treatment of Hirsutism. However, a significant number of patients experience discomfort with reported procedures. Cyperus Rotundus Linn. have reported for its anti-androgenic activity effective against Hirsutism disorder. The main objective of present investigation is to screen the selected phytoconstituents of stated plant against 5 α Reductase Enzyme. In this study Cyperene, humulen, β- selinene, campholenic aldehyde, and α-pinene were docked with 5 α Reductase Enzyme using PyRx 0.8.Autodock and binding energies were obtained. The present investigation concludes that the molecular docking analysis of selected phytoconstituents with 5 α reductase enzyme shows good interaction. The binding affinity of Humulen is higher than others whereas campholenic aldehyde showed lowest affinity amongst all other constituents. Further studies need to be performed at laboratory level to support results of computational screening of present investigation

Screening of Chloroform and Methanolic Bark Extracts of Commiphora caudata for its In-vitro Anti-bacterial, Anti-fungal and In-vivo Anti-inflammatory Activity

Herbal medicines and their preparations have been widely used from the thousands of years in developing and developed countries in the primary health care of society and community. They have great demand due its safety, efficacy with minimum side or adverse effects. Commiphora caudata. It’s known as konda mava in kannada, ikkata in Sanskrit and hill mango in English. It’s widely used in the management of various disorders. Hence the identification of bioactive fractions from various parts of selected medicinal plant is important. In the present research work an attempt has been made to screen and assess the antibacterial, antifungal and anti-inflammatory activities of Commiphora caudata barks. The barks of selected plant material were collected, authenticated, powdered and subjected for extraction procedure. The extracts were screened for presence of various phytoconstituents. The antibacterial activity of chloroform and methanolic extracts were performed against various strains of bacteria and fungi. The extracts also were investigated for its in-vivo anti-inflammatory activity. The result of investigation concludes that chloroform and methanolic extract of plant were potential to inhibit the growth of selected strains of microorganism and also produced potential anti-inflammatory effect