Development of mortar simulator with shell-in-shell system – problem of internal ballistics

The shell-in-shell system used in the mortar simulator raises a number of non-standard technical and computational problems starting from the requirement to distribute the propelling blast energy between the warhead and the ballistic barrel, finishing with the requirement that the length of warhead’s flight path must be scaled to combat shell firing tables. The design problem of the simulator is split into two parts – the problem of external ballistics where the initial velocities of the warhead must be determined, and the problem of internal ballistics – where the design of the cartridge and the ballistic barrel must be performed. Initial velocities of the warhead determined in the problem of external ballistics form the set of initial data for the problem of internal ballistics of mortar simulator with shell-in-shell system. The ballistic barrel (reusable component of the mimicking shell) must be ejected from the mortar tube and its flight path must be only few meters. Moreover, the propelling charge can be located only in the warhead and the blast energy must be distributed between the warhead and the ballistic barrel. That turns the problem of internal ballistics into a complex nonlinear dynamical problem. Its solution involves building of the numerical model, optimisation of system parameters and experimental investigations. Presented mortar simulator proved its effectiveness in combat training exercises and is fully adopted in Lithuanian Army training facilitie

The dynamics of the one-way pneumatic drive for training weapons

Riflemen’s trainers are efficiently applied for the training of the armed forces, police and shooting athletes. However, the training quality directly depends on the perfection of trainers as well as their functional facilities. Trainers should qualitatively develop the skills of correct levelling of a gun to target, represent probable combat situations and reproduce the process of single and shot series. The work presents the theoretical and experimental investigation of the pneumatic drive for recoil imitation of the weapo

Design and research of a laser trainer with all the functions of the G – 36

Riflemen trainers are effectively applied for the training of armed forces, police and shooting athletes. However, the training quality directly depends on the perfection of trainers as well as their functional features. Trainers should qualitatively develop the skills of correct leveling of the G-36 to the target, represent probable combat situations and reproduce the process of single shots and shot series. This research work presents the theoretical and experimental investigation of the pneumatic drive for recoil imitation of the automatic gun G-36 and its employment in the laser riflemen trainers

Development of mortar simulator with shell-in-shell system – problem of internal ballistics

The shell-in-shell system used in the mortar simulator raises a number of non-standard technical and computational problems starting from the requirement to distribute the propelling blast energy between the warhead and the ballistic barrel, finishing with the requirement that the length of warhead’s flight path must be scaled to combat shell firing tables. The design problem of the simulator is split into two parts – the problem of external ballistics where the initial velocities of the warhead must be determined, and the problem of internal ballistics – where the design of the cartridge and the ballistic barrel must be performed. Initial velocities of the warhead determined in the problem of external ballistics form the set of initial data for the problem of internal ballistics of mortar simulator with shell-in-shell system. The ballistic barrel (reusable component of the mimicking shell) must be ejected from the mortar tube and its flight path must be only few meters. Moreover, the propelling charge can be located only in the warhead and the blast energy must be distributed between the warhead and the ballistic barrel. That turns the problem of internal ballistics into a complex nonlinear dynamical problem. Its solution involves building of the numerical model, optimisation of system parameters and experimental investigations. Presented mortar simulator proved its effectiveness in combat training exercises and is fully adopted in Lithuanian Army training facilitie

Development of mortar simulator with shell-in-shell system – problem of internal ballistics

The shell-in-shell system used in the mortar simulator raises a number of non-standard technical and computational problems starting from the requirement to distribute the propelling blast energy between the warhead and the ballistic barrel, finishing with the requirement that the length of warhead’s flight path must be scaled to combat shell firing tables. The design problem of the simulator is split into two parts – the problem of external ballistics where the initial velocities of the warhead must be determined, and the problem of internal ballistics – where the design of the cartridge and the ballistic barrel must be performed. Initial velocities of the warhead determined in the problem of external ballistics form the set of initial data for the problem of internal ballistics of mortar simulator with shell-in-shell system. The ballistic barrel (reusable component of the mimicking shell) must be ejected from the mortar tube and its flight path must be only few meters. Moreover, the propelling charge can be located only in the warhead and the blast energy must be distributed between the warhead and the ballistic barrel. That turns the problem of internal ballistics into a complex nonlinear dynamical problem. Its solution involves building of the numerical model, optimisation of system parameters and experimental investigations. Presented mortar simulator proved its effectiveness in combat training exercises and is fully adopted in Lithuanian Army training facilitie

Human pluripotent stem cell-derived cells endogenously expressing follicle-stimulating hormone receptors : modeling the function of an inactivating receptor mutation

Follicle-stimulating hormone (FSH) is crucial in the development and regulation of reproductive functions. The actions of human FSH and its receptor (FSHR) and mutations therein have mainly been studied using in vivo models, primary cells, cancer cells and cell lines ectopically expressing the FSHR. To allow studies of endogenous FSHR function in vitro, we differentiated FSHR-expressing cells from human pluripotent stem cells. FSH stimulation of the wild-type (WT), but not the inactivating Finnish founder mutant (A189V) receptor, activated the canonical cyclic adenosine monophosphate (cAMP)-dependent signaling pathway and downstream mediators. To investigate protein-protein interaction partners of FSHR at resting state and upon FSH stimulation, we expressed FSHR in HEK293 cells followed by affinity purification mass spectrometry analyses. We found 19 specific high-confidence interacting proteins for WT FSHR and 14 for A189V FSHR, several of which have been linked to infertility. Interestingly, while only WT FSHR interacted with FSH, insulin-like growth factor 1 receptor (IGF1R), for example, interacted with both WT and A189V FSHR upon FSH stimulation. In conclusion, our protocol allows detailed studies of FSH action and disease modeling in human cells endogenously expressing FSHR.Peer reviewe

Human pluripotent stem cell-derived cells endogenously expressing follicle-stimulating hormone receptors : modeling the function of an inactivating receptor mutation

Follicle-stimulating hormone (FSH) is crucial in the development and regulation of reproductive functions. The actions of human FSH and its receptor (FSHR) and mutations therein have mainly been studied using in vivo models, primary cells, cancer cells and cell lines ectopically expressing the FSHR. To allow studies of endogenous FSHR function in vitro, we differentiated FSHR-expressing cells from human pluripotent stem cells. FSH stimulation of the wild-type (WT), but not the inactivating Finnish founder mutant (A189V) receptor, activated the canonical cyclic adenosine monophosphate (cAMP)-dependent signaling pathway and downstream mediators. To investigate protein-protein interaction partners of FSHR at resting state and upon FSH stimulation, we expressed FSHR in HEK293 cells followed by affinity purification mass spectrometry analyses. We found 19 specific high-confidence interacting proteins for WT FSHR and 14 for A189V FSHR, several of which have been linked to infertility. Interestingly, while only WT FSHR interacted with FSH, insulin-like growth factor 1 receptor (IGF1R), for example, interacted with both WT and A189V FSHR upon FSH stimulation. In conclusion, our protocol allows detailed studies of FSH action and disease modeling in human cells endogenously expressing FSHR.Peer reviewe

Human pluripotent stem cell-derived cells endogenously expressing follicle-stimulating hormone receptors: modeling the function of an inactivating receptor mutation

Follicle-stimulating hormone (FSH) is crucial in the development and regulation of reproductive functions. The actions of human FSH and its receptor (FSHR) and mutations therein have mainly been studied using in vivo models, primary cells, cancer cells and cell lines ectopically expressing the FSHR. To allow studies of endogenous FSHR function in vitro, we differentiated FSHR-expressing cells from human pluripotent stem cells. FSH stimulation of the wild-type (WT), but not the inactivating Finnish founder mutant (A189V) receptor, activated the canonical cyclic adenosine monophosphate (cAMP)-dependent signaling pathway and downstream mediators. To investigate protein-protein interaction partners of FSHR at resting state and upon FSH stimulation, we expressed FSHR in HEK293 cells followed by affinity purification mass spectrometry analyses. We found 19 specific high-confidence interacting proteins for WT FSHR and 14 for A189V FSHR, several of which have been linked to infertility. Interestingly, while only WT FSHR interacted with FSH, insulin-like growth factor 1 receptor (IGF1R), for example, interacted with both WT and A189V FSHR upon FSH stimulation. In conclusion, our protocol allows detailed studies of FSH action and disease modeling in human cells endogenously expressing FSHR