Ovarian Dynamic in Ongole Grade Cattle After GnRH Injection in Ovsynch Protocol Based on Progesterone Device

PO cattle have weaknesses to show clear estrus signs which cause difficulty in artificial insemination implementation. The present study was designed to obtain ovarian dynamic as effect of GnRH injection in ovsynch protocol based on progesterone intravaginal device. Heifers (18) and cows (n= 12) were allocated to one of three groups. Cuemate-PGF2α (CP) group inserted with a Cuemate on day 0-7 and injected with prostaglandin on day 7. Cuemate-PGF2α-GnRH (CPG) group was treated as CP group with the addition of GnRH injection on day 9. GnRH-Cuemate-PGF2α-GnRH (GCPG) group was treated as CPG group with addition of GnRH injection on day 0. Ultrasonography was performed on days 0-3, day 7 until ovulation and 7 days after ovulation. Percentage of ovulation synchronization increased significantly (P<0.01) between CP, CPG, and GCPG, respectively, both in heifers (16%, 50%, and 85%, respectively) and cows (0%, 60%, and 100%, respectively), on day 11. Preovulatory follicle diameters between CP, CPG, and GCPG treatments were not different significantly both in heifers (11.9±0.5, 11.9±0.5, and 12.1±0.6 mm, respectively) and cows (11.7±0.4, 11.8±0.7, and 11.1±0.6 mm, respectively). This study concluded that GCPG protocol increased the synchrony of ovulation rate both in cows and heifers, without affecting the follicle preovulatory and CL diameters

PERUBAHAN SEBARAN SEL-SEL ASIDOFIL DAN BASOFIL HIPOFISA PENGARUH PEMBERIAN PASAK BUMI (Eurycoma Longifolia Jack)

Pasak bumi is popular as an aphrodisiac in the traditional medical herb. Boiled drink water of the root powder of pasak bumi is believed can increase the secondary reproduction characters such as libido and the duration of erection. This article is part of the research report that focus on: the distribution of hypophysis acidophil and basophil cells caused of the pasak bumi optimal dose treatment. Research carried out in an experiment using Hemaktosilin Eosin(HE) staining method to study micromorphological distribution of the acidophil and basophil cells of the male rat hypophysis after pasak bumi treatment (the dose of 18 mg pasak bumi/200 g body weight during three days, and the control of 1 ml aquadest). Result of the research showed that: Basophyl cells increased significantly in the third days of pasak bumi administered. Meanwhile acidophyl cells decreased in the third days of pasak bumi administered. &nbsp

Assessment of Fertility Status in the Male Sumatran Rhino at the Sumateran Rhino Sanctuary, Way Kambas National Park, Lampung

Sumatran rhino is the most endangered rhino species. Its population is estimated less than 300 individuals remaining in the wild with highly declining rate to 50% in the last 15 years. The number of male rhinoceroses in the captivity are very few, therefore the assessment of its fertility is very important in order to support the breeding success since the captive breeding success is very poor. The objectives of this study were (i) to determine the male reproductive status, (ii) to establish a reliable semen collection method, and (iii) to assess semen parameters of the fresh collected sample. Three methods of semen collection were examined to determine its fertilizing potential, i.e. (i) stimulated combination of artificial vagina (AV), penile massage (PM) and accessory gland massage (AGM); (ii) AV and PM; and (iii) only with PM. The first method gave the best result with an ejaculation success of 85.71% (6/7, n = 7). The second and third methods obtained an ejaculation success rate of 50% (2/4, n = 4)) and 25% (1/4, n = 4), respectively. The collected ejaculates had a volume of 1.2-12.4 ml with whitish to cream turbid colour and pH 6.90-6.99. Sperm concentration was (143-333) x 103 sperm/ml. The quality of the sperm was low with only approximately 1% of them moved forward slowly. Approximately 80% of the spermatozoa were immature (prox. cytoplasmic droplet) with head (macro-, microcephalic) and tail abnormalities (broken tail). Semen quality increased after several collections and the amount of immature sperm decreased up to 5%. Electroejaculation procedure could produce 34 ml semen, but no sperm was found in the ejaculate. Hence, the combination of AV, PM, and AGM could get higher volume of ejaculate compared to other methods, but sperm concentration was better obtained using AV and PM only. Repeated semen collection increased semen quality, although the male has low fertilizing capacity due to low sperm concentration (oligozoospermia) and small volume of the ejaculate (oligospermia). Key words: Sumatran rhino, semen, sperms, collectio

Ovarian Dynamic in Ongole Grade Cattle after GnRH Injection in Ovsynch Protocol Based on Progesterone Device

PO cattle have weaknesses to show clear estrus signs which cause difficulty in artificial insemination implementation. The present study was designed to obtain ovarian dynamic as effect of GnRH injection in ovsynch protocol based on progesterone intravaginal device. Heifers (18) and cows (n= 12) were allocated to one of three groups. Cuemate-PGF2α (CP) group inserted with a Cuemate on day 0-7 and injected with prostaglandin on day 7. Cuemate-PGF2α-GnRH (CPG) group was treated as CP group with the addition of GnRH injection on day 9. GnRH-Cuemate-PGF2α-GnRH (GCPG) group was treated as CPG group with addition of GnRH injection on day 0. Ultrasonography was performed on days 0-3, day 7 until ovulation and 7 days after ovulation. Percentage of ovulation synchronization increased significantly (P<0.01) between CP, CPG, and GCPG, respectively, both in heifers (16%, 50%, and 85%, respectively) and cows (0%, 60%, and 100%, respectively), on day 11. Preovulatory follicle diameters between CP, CPG, and GCPG treatments were not different significantly both in heifers (11.9±0.5, 11.9±0.5, and 12.1±0.6 mm, respectively) and cows (11.7±0.4, 11.8±0.7, and 11.1±0.6 mm, respectively). This study concluded that GCPG protocol increased the synchrony of ovulation rate both in cows and heifers, without affecting the follicle preovulatory and CL diameters.Key words: ovarian dynamic, GnRH, ovsych, PO Cattl

Perubahan Morfo-anatomi dan Penyimpanan Energi pada Fase Perkembangan Gonad Ikan Senggaringan, Mystus Nigriceps (Valenciennes, 1840) di Sungai Klawing Purbalingga, Jawa Tengah [Morpho-anatomical Changes And Energy Storage During Gonadal Development Of Twospots Catfish, Mystus Nigriceps (Valenciennes, 1840) In Klawing River, Purbalingga, Central Java]

Suatu penelitian dengan tujuan untuk mengkaji Perubahan nilai indeks morfo-anatomi dan penyimpanan energi pada beberapa organ tubuh ikan senggaringan selama masa perkembangan gonad telah dilaksanakan di Sungai Klawing, Purbalingga, Jawa Tengah. Parameter morfo-anatomi meliputi faktor kondisi (CF), indeks jaringan viseral (VSI), indeks sirip lemak (AFI), indeks jaringan hati (HSI), indeks jaringan gonad (GSI), dan kandungan energi pada organ/jaringan otot punggung, sirip lemak, organ viseral, hati, dan gonad telah diukur berdasarkan tingkat perkembangan gonadnya. Hasil penelitian menunjukkan bahwa selama masa perkembangan gonad terjadi Perubahan nilai-nilai parameter morfo-anatomi dan kandungan energi pada organ yang diukur, terjadi proses penyimpanan, perpindahan dan Perubahan materi berenergi baik untuk keperluan pematangan gonad maupun untuk aktivitas pemijahan

PENDUGAAN UKURAN PERTAMA KALI MATANG GONAD IKAN SENGGARINGAN (Mystus negriceps) DI SUNGAI KLAWING, PURBALINGGA JAWA TENGAH

ABSTRACT Fish reproduction aspect must be know to improve some fish to be broodstock, like as food needed, water quality etc.  The other aspect must be know is how long body size at the first time maturity.  Size body at the first time maturity in fish is important information, because this information will give us how long in body some fish begin done reproduction. This research was conducted in Klaving river, Purbalingga residence of Midle Java, fishes were sampled twice a month (March to December). The variable measured were total length where fish reach gonad maturity on 4th level. The result of this research was shown that 148.9010 mm of body size for Mystus negriceps reach the first time maturity. Keywords : reproduction, first time maturity, Klawing rive

Potensi Alfa Enolase (ENO1) Membran Plasma Spermatozoa Sapi Bali Sebagai protein Antigenik

Antibodi imunoglobulin G (IgG) merupakan salah satu immunoglobulin yang dikandung oleh antibodi anti-sperma (ASA) yang terdapat pada saluran reproduksi betina. Imunoglobulin G dapt berikatan dengan protein-protein yang berpotensi sebagai protein antigenik seperti alfa enolase (ENO1). Penelitian ini bertujuan untuk mengidentifikasi dinamika dan potensi ENO1 membran plasma spermatozoa sebagai protein antigenik serta kualitas spermatozoa sapi Bali dengan perlakuan 0,5 mg/ml IgG. Sampel penelitian adalah 16 ejakulat yang diperoleh dari 4 ekor pejantan. Motilitas spermatozoa dievaluasi menggunakan CASA, viabilitas melalui metode pewarnaan diferensial, Keutuhan Membran Plasma (MPU) menggunakan metode Hypo-osmotic Swelling Test (HOS-Test), nilai Mix Anti-globulin Reaction (MAR) diperoleh dari MAR-Test, sedangkan kuantitas ENO1 diukur dengan ELISA. Analisis data menggunakan analisis ragam RAK. Hasil penelitian menunjukkan bahwa perlakuan dengan IgG sampai 0,5 mg/ml menurunkan motilitas, kinematika spermatozoa, viabilitas, MPU dan kuantitas ENO1 membran plasma spermatozoa (P<0,01), serta meningkatkan nilai MAR-test (P<0,05). Penelitian menyimpulkan bahwa IgG dapat menurunkan kualitas spermatozoa dan kuantitas ENO1, sekaligus menunjukkan bahwa ENO1 membran plasma spermatozoa sapi Bali berpotensi sebagai protein antigenik

Koleksi Sel Telur dengan Teknik Laparoskopi untuk Produksi Embrio dan Transfer Embrio pada Domba

An experiment was carried out to analyze the application of laparoscopic technique for oocyte collection, in vitro embryo production and embryo transfer in sheep. The first experiment was conducted to observe effect of gonadotropin stimulation on follicle development and laparoscopic technique for oocytes aspiration. In the second experiment, effect of culture system on the embryo development in vitro was assessed and in the third experiment, the application of laparoscopic for embryo transfer has been conducted. The result showed that single dose of gonadotrophin was sufficient to support follicle development significantly and it could help follicle visualization. It also showed that laparoscopic ovum-pick up could be conducted weekly without any restriction The second series experiment showed CR1aa culture system was better than TCM 199 (29.90°/o vs 8.00%) and the changing of media was required to ensure better metabolism process for embryos. The third experiment revealed that embryo transfer could be conducted with an aid from laparoscope. In conclusion, single dose PMSG stimulation is sufficient to support follicle development for /aparoscopic ovum-pick up, the culture media changing affects the successful rate of in vitro embryo production (8% vs 25.66%) and the laparoscopy technique can be used safely for embryo transfer on sheep.Keyword: laparoscopic, oocyte, embryo transfer, shee

Respons dan Karakteristik Estrus setelah Sinkronisasi Estrus dengan Cloprostenol pada Sapi Friesian Holstein

Penelitian tentang sinkronisasi estrus pada sapi perah Friesian Holstein (FH) menggunakan cloprostenol telah dilakukan. Penelitian ini bertujuan untuk mengamati respons dan karakteristik estrus serta pengukuran nilai hambatan arus listrik lendir vagina. Penelitian dilakukan pada 20 ekor sapi FH dengan dua perlakuan injeksi cloprostenol, yaitu injeksi tunggal dengan keberadaan corpus luteum dan injeksi ganda dengan selang waktu injeksi 11 hari. Pengamatan estrus dan pengukuran lendir dilakukan lima hari berturut-turut setelah injeksi cloprostenol yang terakhir. Parameter pengukuran yang diamati meliputi respons, onset, dan durasi estrus, serta nilai hambatan arus listrik lendir vagina. Hasil penelitian menunjukkan bahwa injeksi ganda menghasilkan respons estrus yang lebih baik dibandingkan injeksi tunggal (90% vs 70%), dengan onset estrus lebih pendek (47,55 jam vs 53,28 jam), dan durasi estrus lebih pendek (24,85 jam vs 23,55 jam). Data hambatan arus listrik lendir vagina pada kelompok injeksi ganda lebih rendah dibandingkan dengan injeksi tunggal (187,77 vs 192,14), dengan pola tinggi sebelum estrus, rendah pada saat estrus, dan tinggi kembali setelah estrus, baik pada kelompok injeksi tunggal maupun injeksi ganda. Dapat disimpulkan bahwa respons estrus pada kelompok injeksi ganda lebih baik dibandingkan dengan kelompok injeksi tunggal

Koleksi Sel Telur dengan Teknik Laparoskopi untuk Produksi Embrio dan Transfer Embrio pada Domba

An experiment was carried out to analyze the application of laparoscopic technique for oocyte collection, in vitro embryo production and embryo transfer in sheep. The first experiment was conducted to observe effect of gonadotropin stimulation on follicle development and laparoscopic technique for oocytes aspiration. In the second experiment, effect of culture system on the embryo development in vitro was assessed and in the third experiment, the application of laparoscopic for embryo transfer has been conducted. The result showed that single dose of gonadotrophin was sufficient to support follicle development significantly and it could help follicle visualization. It also showed that laparoscopic ovum-pick up could be conducted weekly without any restriction The second series experiment showed CR1aa culture system was better than TCM 199 (29.90°/o vs 8.00%) and the changing of media was required to ensure better metabolism process for embryos. The third experiment revealed that embryo transfer could be conducted with an aid from laparoscope. In conclusion, single dose PMSG stimulation is sufficient to support follicle development for /aparoscopic ovum-pick up, the culture media changing affects the successful rate of in vitro embryo production (8% vs 25.66%) and the laparoscopy technique can be used safely for embryo transfer on sheep.Keyword: laparoscopic, oocyte, embryo transfer, shee