DUKKU AND BAEKAM SPRINGWATER INHIBIT THE UREASE ACTIVITY OF HELICOBACTER PYLORI

Background: Springwater (SW) contains many kinds of minerals such as sodium, potassium and copper. These metallic ions may influence the activity of metallo-enzymes such as urease via competitive inhibition. In this study, we investigated the effect of SW on the inhibition of Ni-containing urease activity, which is essential for the colonization of Helicobacter pylori (H. pylori) in the human stomach.Materials and Methods: We studied the growth inhibition of H. pylori by SW. We evaluated ammonia production to detect urease activity and performed western blot analysis of UreA and UreB for enzyme production.Results: SW had no significant effect on bacterial growth. Western blot analysis also showed that SW did not affect the translation of UreA and UreB, but it significantly reduced the urease activities of the Jack bean as well as that of H.pylori from 50 to 75%.Conclusion: These results might indicate that the consumption of SW may prevent the colonization of H. pylori andameliorate the toxic effect on gastric mucosa via the inhibition of urease activity

ニホンザル ノ セイソウ トクイテキ タンパクシツ , MFSJ1 オヨビ PRM2 : cDNA ノ molecular cloning コウゾウ ブンセキ ハッタツ ト ハンショク キセツ ニ ヨル ハツゲン ヘンカ

京都大学0048新制・課程博士博士(理学)甲第8800号理博第2372号新制||理||1232(附属図書館)UT51-2001-F130京都大学大学院理学研究科生物科学専攻(主査)教授 竹中 修, 教授 景山 節, 教授 松林 清明学位規則第4条第1項該当Doctor of ScienceKyoto UniversityDA