4,726 research outputs found

    Onset of unsteady horizontal convection in rectangle tank at Pr=1Pr=1

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    The horizontal convection within a rectangle tank is numerically simulated. The flow is found to be unsteady at high Rayleigh numbers. There is a Hopf bifurcation of RaRa from steady solutions to periodic solutions, and the critical Rayleigh number RacRa_c is obtained as Rac=5.5377×108Ra_c=5.5377\times 10^8 for the middle plume forcing at Pr=1Pr=1, which is much larger than the formerly obtained value. Besides, the unstable perturbations are always generated from the central jet, which implies that the onset of instability is due to velocity shear (shear instability) other than thermally dynamics (thermal instability). Finally, Paparella and Young's [J. Fluid Mech. 466 (2002) 205] first hypotheses about the destabilization of the flow is numerically proved, i.e. the middle plume forcing can lead to a destabilization of the flow.Comment: 4pages, 6 figures, extension of Chin. Phys. Lett. 2008, 25(6), in pres

    Application of Neural-Like P Systems With State Values for Power Coordination of Photovoltaic/Battery Microgrids

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    The power coordination control of a photovoltaic/battery microgrid is performed with a novel bio-computing model within the framework of membrane computing. First, a neural-like P system with state values (SVNPS) is proposed for describing complex logical relationships between different modes of Photovoltaic (PV) units and energy storage units. After comparing the objects in the neurons with the thresholds, state values will be obtained to determine the con guration of the SVNPS. Considering the characteristics of PV/battery microgrids, an operation control strategy based on bus voltages of the point of common coupling and charging/discharging statuses of batteries is proposed. At rst, the SVNPS is used to construct the complicated unit working modes; each unit of the microgrid can adjust the operation modes automatically. After that, the output power of each unit is reasonably coordinated to ensure the operation stability of the microgrid. Finally, a PV/battery microgrid, including two PV units, one storage unit, and some loads are taken into consideration, and experimental results show the feasibility and effectiveness of the proposed control strategy and the SVNPS-based power coordination control models

    Experimental Observation of Classical Sub-Wavelength Interference with Thermal-Like Light

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    We show the experimental observation of the classical sub-wavelength double-slit interference with a pseudo-thermal light source. The experimental results are in agreement with the recent theoretical prediction shown in quant-ph/0404078 (to be appeared in Phys. Rev. A).Comment: 4 pages, 6 figure

    {4-Chloro-2-[(2-hy­droxy­eth­yl)imino­meth­yl]phenolato}{4-chloro-2-[(2-oxido­eth­yl)imino­meth­yl]phenolato}cobalt(III)

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    In the title mononuclear cobalt(III) compound, [Co(C9H8ClNO2)(C9H9ClNO2)], the CoII atom is six-coordinated by two imine N atoms, two phenolate O atoms, and one hy­droxy and one oxide O atom from two Schiff base ligands, forming an octa­hedral geometry. In the crystal structure, adjacent mol­ecules are linked through inter­molecular O—H⋯O hydrogen bonds. The 2-oxidoethyl group is disordered over two positions in a 0.638 (3):0.362 (3) ratio

    The chloride channel cystic fibrosis transmembrane conductance regulator (CFTR) controls cellular quiescence by hyperpolarizing the cell membrane during diapause in the crustacean Artemia

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    Cellular quiescence, a reversible state in which growth, proliferation, and other cellular activities are arrested, is important for self-renewal, differentiation, development, regeneration, and stress resistance. However, the physiological mechanisms underlying cellular quiescence remain largely unknown. In the present study, we used embryos of the crustacean Artemia in the diapause stage, in which these embryos remain quiescent for prolonged periods, as a model to explore the relationship between cell-membrane potential (V-mem) and quiescence. We found that V-mem is hyperpolarized and that the intracellular chloride concentration is high in diapause embryos, whereas V-mem is depolarized and intracellular chloride concentration is reduced in postdiapause embryos and during further embryonic development. We identified and characterized the chloride ion channel protein cystic fibrosis transmembrane conductance regulator (CFTR) of Artemia (Ar-CFTR) and found that its expression is silenced in quiescent cells of Artemia diapause embryos but remains constant in all other embryonic stages. Ar-CFTR knockdown and GlyH-101-mediated chemical inhibition of Ar-CFTR produced diapause embryos having a high V-mem and intracellular chloride concentration, whereas control Artemia embryos released free-swimming nauplius larvae. Transcriptome analysis of embryos at different developmental stages revealed that proliferation, differentiation, and metabolism are suppressed in diapause embryos and restored in postdiapause embryos. Combined with RNA sequencing (RNA-Seq) of GlyH-101-treated MCF-7 breast cancer cells, these analyses revealed that CFTR inhibition down-regulates the Wnt and Aurora Kinase A (AURKA) signaling pathways and up-regulates the p53 signaling pathway. Our findings provide insight into CFTR-mediated regulation of cellular quiescence and V-mem in the Artemia model

    Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2

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    <p>Abstract</p> <p>Background</p> <p>The capsid protein (ORF2) is a major structural protein of porcine circovirus type 2 (PCV2). A simple and reliable diagnostic method based on ORF2 protein immunoreactivity would serve as a valuable diagnostic method for detecting serum antibodies to PCV2 and monitoring PCV infection. Here, we reported an indirect enzyme-linked immunosorbent assay (I-ELISA) by using an antigenic domain (113-147AA) of ORF2-encoded antigen, expressed in <it>E. coli</it>, for diagnosis of PCV infection.</p> <p>Results</p> <p>The ELISA was performed on 288 serum samples collected from different porcine herds and compared with an indirect immunofluorescent assay (IFA). In total, 262 of 288 samples were positive as indicated by both I-ELISA and IFA. The specificity and sensitivity of I-ELISA were 87.7% and 93.57%.</p> <p>Conclusions</p> <p>This ELISA is suitable for detection and discrimination of PCV2 infection in both SPF and farm antisera.</p
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