Impact of lysogeny on bacteria with a focus on lactic acid bacteria

Lysogeny is a widespread occurrence in bacteria and represents a highly co-evolved adaptive state between bacteriophages and their hosts. Different lysogenic conversion phenomena have been described for various bacterial species. This review outlines recent progress on the molecular analysis of bacteriophage lysogenic conversion, immunity systems, temperate phageencoded phage resistance, as well as transduction. Since phage-host interactions in lactic acid bacteria (LAB) are commercially relevant and have received a lot of scientific attention in recent years, a special focus will be given to the impact of lysogeny in LAB

A Case Study on Foamy Oil Characteristics of the Orinoco Belt, Venezuela

With a current recovery of less than 11%, the Orinoco Belt in Venezuela still contains potentially more than 1.3 trillion barrels of reserves of “three highs, one low” oil at a depth of 100 to 1500 m. 5 joint projects and one project of Petroleos de Venezuela SA are making plans to improve oil recovery in the area. So it is important for them to have a thorough knowledge of foamy oil characteristics. This reservoir has a peculiar behavior called as a foamy phenomenon. In order to characterize the properties of the foamy oil, this paper discussed unconventional test methodology and the detailed suite of laboratory procedures including PVT and pressure depletion tests used to examine the Orinoco heavy oil. The results showed substantial differences in characteristics of foamy oil and conventional oil studied, not only in terms of PVT behavior but also in terms of the production performance during pressure depletion tests. The foamy oil compressibility was between 10-120×10-4 mPa-1, which was obviously higher than that of conventional oil. Differential liberation experiments of the oil, with obvious high formation volume factor, stable GOR, and low density showed a strong tendency to foam below the bubble point. Other notable observations were that more efficient oil recovery was achieved at high depletion rates while less free gas was produced.Key words: Foamy oil; Unconventional tests; The Orinoco Belt; PVT; Pressure depletion test

A tetraspecific VHH-based neutralizing antibody modifies disease outcome in three animal models of Clostridium difficile infection

Clostridium difficile infection (CDI), a leading cause of nosocomial infection, is a serious disease in North America, Europe, and Asia. CDI varies greatly from asymptomatic carriage to life-threatening diarrhea, toxic megacolon, and toxemia. The incidence of community-acquired infection has increased due to the emergence of hypervirulent antibiotic-resistant strains. These new strains contribute to the frequent occurrence of disease relapse, complicating treatment, increasing hospital stays, and increasing morbidity and mortality among patients. Therefore, it is critical to develop new therapeutic approaches that bypass the development of antimicrobial resistance and avoid disruption of gut microflora. Here, we describe the construction of a single heteromultimeric VHH-based neutralizing agent (VNA) that targets the two primary virulence factors of Clostridium difficile, toxins A (TcdA) and B (TcdB). Designated VNA2-Tcd, this agent has subnanomolar toxin neutralization potencies for both C. difficile toxins in cell assays. When given systemically by parenteral administration, VNA2-Tcd protected against CDI in gnotobiotic piglets and mice and to a lesser extent in hamsters. Protection from CDI was also observed in gnotobiotic piglets treated by gene therapy with an adenovirus that promoted the expression of VNA2-Tcd

Impact of CodY protein on metabolism, sporulation and virulence in Clostridioides difficile ribotype 027

Toxin synthesis and endospore formation are two of the most critical factors that determine the outcome of infection by Clostridioides difficile. The two major toxins, TcdA and TcdB, are the principal factors causing damage to the host. Spores are the infectious form of C. difficile, permit survival of the bacterium during antibiotic treatment and are the predominant cell form that leads to recurrent infection. Toxin production and sporulation have their own specific mechanisms of regulation, but they share negative regulation by the global regulatory protein CodY. Determining the extent of such regulation and its detailed mechanism is important for understanding the linkage between two apparently independent biological phenomena and raises the possibility of creating new ways of limiting infection. The work described here shows that a codY null mutant of a hypervirulent (ribotype 027) strain is even more virulent than its parent in a mouse model of infection and that the mutant expresses most sporulation genes prematurely during exponential growth phase. Moreover, examining the expression patterns of mutants producing CodY proteins with different levels of residual activity revealed that expression of the toxin genes is dependent on total CodY inactivation, whereas most sporulation genes are turned on when CodY activity is only partially diminished. These results suggest that, in wild-type cells undergoing nutrient limitation, sporulation genes can be turned on before the toxin genes

Disentangling the effects of vapor pressure deficit on northern terrestrial vegetation productivity

The impact of atmospheric vapor pressure deficit (VPD) on plant photosynthesis has long been acknowledged, but large interactions with air temperature (T) and soil moisture (SM) still hinder a complete understanding of the influence of VPD on vegetation production across various climate zones. Here, we found a diverging response of productivity to VPD in the Northern Hemisphere by excluding interactive effects of VPD with T and SM. The interactions between VPD and T/SM not only offset the potential positive impact of warming on vegetation productivity but also amplifies the negative effect of soil drying. Notably, for high-latitude ecosystems, there occurs a pronounced shift in vegetation productivity\u27s response to VPD during the growing season when VPD surpasses a threshold of 3.5 to 4.0 hectopascals. These results yield previously unknown insights into the role of VPD in terrestrial ecosystems and enhance our comprehension of the terrestrial carbon cycle\u27s response to global warming

A Novel Bacteriophage Lysin-Human Defensin Fusion Protein Is Effective in Treatment of Clostridioides difficile Infection in Mice

Clostridioides difficile is the leading cause of worldwide antibiotics-associated diarrhea. In this study, we report the construction and evaluation of a novel bacteriophage lysin-human defensin fusion protein targeting C. difficile. The fusion protein, designated LHD, is composed of two parts connected by a 3-repeating unit linker “(GGGGS)3”: the catalytic domain of a lysin protein from a C. difficile bacteriophage phiC2 (LCD), and the functional domain of a human defensin protein HD5. Lytic assays showed that LHD protein had a potent lytic activity against different types of clinical C. difficile strains, including the epidemic 027, 078, 012, and 087 strains. The minimum inhibitory concentration (MIC) of LHD was 0.78 μg/ml, which was lower than the MIC of the protein LCD (1.56 μg/ml), and the MICs of metronidazole (4 μg/ml) and vancomycin (4 μg/ml). In addition, the LHD protein could lyse C. different strains in different pHs (6.0, 7.0, and 8.0). Evaluation of LHD potency in vivo using mouse model of C. difficile infection (CDI) showed that administration of the LHD protein (twice daily for 7 days) was effective in mitigating the symptoms and reducing the death from CDI. Treatment with LHD also significantly decreased the number of C. difficile spores and the toxin level in feces from the infected mice. Our data suggest that this novel lysin-human defensin fusion protein has a potential on CDI control

Rapamycin is highly effective in murine models of immune-mediated bone marrow failure

Acquired aplastic anemia, the prototypical bone marrow failure disease, is characterized by pancytopenia and marrow hypoplasia. Most aplastic anemia patients respond to immunosuppressive therapy, usually with anti-thymocyte globulin and cyclosporine, but some relapse on cyclosporine withdrawal or require long-term administration of cyclosporine to maintain blood counts. In this study, we tested efficacy of rapamycin as a new or alternative treatment in mouse models of immune-mediated bone marrow failure. Rapamycin ameliorated pancytopenia, improved bone marrow cellularity, and extended animal survival in a manner comparable to the standard dose of cyclosporine. Rapamycin effectively reduced Th1 inflammatory cytokines interferon-γ and tumor necrosis factor-α, increased the Th2 cytokine interleukin-10, stimulated expansion of functional regulatory T cells, eliminated effector CD8+ T cells (notably T cells specific to target cells bearing minor histocompatibility antigen H60), and preserved hematopoietic stem and progenitor cells. Rapamycin, but not cyclosporine, reduced the proportion of memory and effector T cells and maintained a pool of naïve T cells. Cyclosporine increased cytoplasmic nuclear factor of activated T-cells-1 following T-cell receptor stimulation, whereas rapamycin suppressed phosphorylation of two key signaling molecules in the mammalian target of rapamycin pathway, S6 kinase and protein kinase B. In summary, rapamycin was an effective therapy in mouse models of immune-mediated bone marrow failure, acting through different mechanisms to cyclosporine. Its specific expansion of regulatory T cells and elimination of clonogenic CD8+ effectors support its potential clinical utility in the treatment of aplastic anemia

Comparison of soil erosion models used to study the Chinese Loess Plateau

The Loess Plateau suffers from severe soil erosion that leads to a series of ecological and economic problems such as reduced land productivity, exacerbated rural poverty, decreased biodiversity and sedimentation of the riverbed in the lower reaches of the Yellow River. Soil erosion models are commonly used on the Loess Plateau to help target sustainable land management strategies to control soil erosion. In this study, we compared eleven soil erosion models that were previously used on the Loess Plateau. We studied their prediction accuracy, process representation, data and calibration requirements, and potential application in scenario studies. The selected models consisted of a broad range of model types, structures and scales. The comparison showed that process-based and empirical models did not necessarily yield more accurate results over one another for the Loess Plateau. Among the process-based models, Si’ model, WEPP and MMF had the highest prediction accuracy. However, some of the selected models were tested with total sediment load while others were tested with suspended sediment load (i.e. bedload is not included), which is subject to several drawbacks. Research questions that each of the models can address on the Loess Plateau were suggested. Further improvement of soil erosion models for the Loess Plateau should concentrate on enhancing the quality of data for model implementation and testing, incorporating key processes into process-based models according to their aims and scales, comparing models that address the same research questions, and implementing internal and spatial model testing