Cell-free synthesis of herpes simplex virus proteins

Journal ArticlePolyribosomes isolated from herpes simplex virus type I (HSV-1)-infected cells have been used to program a eucaryotic cell-free translation system. At least 10 HSV-specific polypeptides, with apparent molecular weights of 25,000 to 160,000, are synthesized by wild-type HSV-infected polyribosomes. Polyribosomes prepared from thymidine kinase-negative mutants of HSV direct the synthesis of three putative nonsense termination polypeptides. HSV-specific polypeptides synthesized in vitro are precipitated with antiserum to HSV-infected cell proteins

Comparative Life History Adaptations of Some Myopsid and Sepiolid Squid

The inadequacies of population data in the determination of squid life history models is discussed. A close, functional comparison is noted for myopsids (Loligo pealei in particular) and the sepiolid squids. Though the latter are small andstrictly nekto-benthic, they are capable of laboratory cultivation and provide excellent models for experimentation. It is now possible to test the physiologicalchanges which support the seasonality of Mesnil's model and to examine the roleof elective spawnin (as proposed here) and social interactions. A qualitative myopsid-sepiolid life cycle summary is presented to illustrate present ecological knowledge on this subject. The subject is briefly discussed in terms of squid fishery management in general

A guide to laboratory use of the squid Loligo pealei

From the Preface: Unfortunately, a guide like this one does not happen simply. It is the product of polite requests (at first polite, anyway) from colleagues, of mutual nagging, and of wives with persistant memories. What we are attempting to achieve with this contribution is a consolidation of many years of personal experience with squid into a source of practical information hopefully useful to the increasing number of people working with the squid. One does not undertake the task of preparing such a guide for a limited audience with unreserved enthusiasm. It always seems the same amount of work could produce something for a larger audience and therefore, hopefully, of greater significance. On the other hand when the hours and days spent on developing handling procedures, techniques, and "tricks of the squid trade" are considered it seems untenable not to pass this otherwise unpublishable data on in hope of saving others like pain and time. Therefore, what we have attempted to do is informally put together information that should prove useful to people interested in all aspects of squid biology. In this way we hope to make the laboratory utilization of the squid more efficient not only from the standpoint of the investigator and the collector but also for the future of the species Loligo pealei