Knowledge attitude and practices of pneumococcal vaccines in India

Introduction: Pneumococcal conjugate vaccines (PCV) have revolutionized the prevention of invasive pneumococcal disease (IPD) globally.The initial 7-valent (PCV7) vaccine has gradually been replaced by higher valency vaccines such as PCV10 and PCV13, as these cover additionalserotypes causing increased immunization. Objective: The purpose of this study was to determine the overall attitude and practices of the Indian public toward PCV for their infants. Materials and Methods: Data were collected over a period of 18 months from a tertiary perinatal center. Pneumococcal vaccine leaflets with current, unbiased information on PCV10 and PCV13 were circulated to parents, who were then asked to opt for either one or none of the vaccines for their infants at their 6 weeks immunization check-up. Results: A total of 3406 infants came for follow-up. Among them, 84% chose to give the PCV while the remaining 16% opted out. Among the parents that opted for the vaccines, 90% chose PCV13 over PCV10. This may be attributable to its increased immunogenicity and broader serotype coverage as understood by the parents - who seem to want the best for their children. Conclusion: PCV13 was a preferred vaccine of choice for infants by majority of the parents, as compared to PCV10. Such information is of valuable importance in deciding which vaccine to go for if and when India goes for PCV in the future with Global Alliance for Vaccines and Immunization and United Nations Children’s Fund funding

Non-Gaussianity Consistency Relation for Multi-field Inflation

While detection of the "local form" bispectrum of primordial perturbations would rule out all single-field inflation models, multi-field models would still be allowed. We show that multi-field models described by the $\delta N$ formalism obey an inequality between $f_{\rm NL}$ and one of the local-form {\it trispectrum} amplitudes, $\tau_{\rm NL}$, such that $\tau_{\rm NL}>\frac12(\frac65f_{\rm NL})^2$ with a possible logarithmic scale dependence, provided that 2-loop terms are small. Detection of a violation of this inequality would rule out most of multi-field models, challenging inflation as a mechanism for generating the primoridal perturbations.Comment: 5 pages. Accepted for publication in Physical Review Letter

Treatment of the microfilaraemia of asymptomatic brugian filariasis with single doses of ivermectin, diethylcarbamazine or albendazole, in various combinations

Several new chemotherapeutic tools are now available for the control of lymphatic filariasis. Combinations of single doses of antifilarial drugs are generally superior to single drugs. The efficacy and safety of albendazole in combination with diethylcarbamazinc (DEC) or ivermectin, for the treatment of Brugia malayi infection, were investigated, for the first time, in an open, hospital-based study. Fifty-one asymptomatic microfilaraemics (with 108-4034 microfilariae/ml; median = 531) of both sexes and aged 14-70 years were randomly allocated to receive single-dose treatments of ivermectin (200 μg/kg) with diethylcarbamazine (DEC; 6 mg/kg), ivermectin (200 μg/kg) with albendazole (400 mg), DEC (6 mg/kg) with albendazole (400 mg), or albendazole (400 mg) alone. Albendazole alone had no effect on the microfilarial levels at the 1-year follow-up but both groups given DEC had significantly lower microfilaraemias ( P < 0.015 and P < 0.02) than that given ivermectin with albendazole. Overall, 47%-64% of those given DEC but only 14% of those given ivermectin with albendazole appeared to be amicrofilaraemic 1 year post-treatment. The adverse reactions seen in the study were mild, transient and qualitatively similar to those seen earlier with ivermectin and DEC. The combination of DEC and albendazole, both well tested drugs, offers a new option for countries such as India where there is no onchocerciasis or loiasis and where ivermectin may not be immediately available. The direct and indirect effects of albendazole on intestinal helminths would be additional benefits

Leadership Qualities Among Women Leaders in Educational Institutions at Bangalore City

Purpose: First, it seeks to investigate the factors that influence women's leadership traits within academic institutions located in Bangalore. Second, it aims to gain a deeper understanding of the evolving role of women in leadership positions within modern society. &nbsp; Design/Methodology/Approach: To achieve its objectives, this study employs a qualitative research approach. Researchers conduct interviews and surveys within academic institutions in Bangalore. By gathering and analyzing this data, they intend to uncover key themes and patterns that shed light on the factors impacting women's leadership traits. &nbsp; Findings: The findings of this research reveal several noteworthy insights. It becomes evident that women's leadership traits are significantly influenced by societal perceptions and economic circumstances. Furthermore, the progression of women in their professional lives has played a crucial role in altering societal views. The study also acknowledges the importance of India's long-standing policy efforts to promote women's development. Additionally, it highlights the remarkable achievements of women in various fields, including academia and athletics. &nbsp; Research, Practical &amp; Social implications: In terms of implications, this study carries significance on multiple fronts. From a research standpoint, it offers valuable insights into the factors that shape women's leadership roles within academic institutions. Practically, the findings can inform strategies and initiatives aimed at promoting and enhancing women's leadership within the educational sector. On a broader social level, the study underscores the evolving role of women in Indian society, emphasizing the importance of gender equality as a key societal value. &nbsp; Originality/Value: In terms of originality, this research distinguishes itself by focusing on the specific context of academic institutions in Bangalore. Its value lies in its contribution to a deeper understanding of women's leadership within a culturally diverse context, shedding light on the unique factors at play in this region

Toxicity and neurophysiological impacts of three plant-derived essential oils against the vineyard mealybug Planococcus ficus

Many natural products are able to control pests and can be used as alternatives for chemical treatments. Plant essential oils (EOs) have been found to exhibit some biological activity against many insects including mealybugs. This study aimed at studying the insecticidal activity and behavioral and neurophysiological impacts of three plant essential oils against the vine mealybug Planococcus ficus. The topical and fumigant toxicity of Cymbopogon citratus, Mentha piperita, and Pelargonium graveolens essential oils was evaluated against P. ficus adults. The chemical composition analysis of EOs by gas chromatographic-mass spectrometry (GC-MS) revealed citronellal (31.69 %), menthol (73.78 %), and geraniol (39.6%), as major components, respectively. Bioassays of EOs against vine mealybug adults through fumigation toxicity method revealed lethal concentrations LC50 values of 17.01, 26.27 and 24.52 µL·L-1 air for C. citratus, M. piperita, and P. graveolens, respectively. In both topical and fumigant bioassays, essential oil from C. citratus was the most active altering the behavioral response of treated mealybugs which becomes hyperactive and disoriented. EOs induced general stress in P. ficus adults, as evidenced by oxidative stress biomarker analyses. Biochemical analyses showed that the EOs exposure reduced the activity of acetylcholinesterase and significantly induced the glutathione S-transferases and Malondialdehydes accumulation in the vine mealybug tissues. Mortality caused by lemongrass EO positively correlated with the significant decrease in the AChE activity indicating lethal neurological effects. These toxicity bioassays and neurological impact findings provide new informations for formulating effective essential oil based-insecticides to control P. ficus in the framework of integrated pest management programs

Inhibition of NAADP signalling on reperfusion protects the heart by preventing lethal calcium oscillations via two-pore channel 1 and opening of the mitochondrial permeability transition pore

Aims In the heart, a period of ischaemia followed by reperfusion evokes powerful cytosolic Ca2+ oscillations that can cause lethal cell injury. These signals represent attractive cardioprotective targets, but the underlying mechanisms of genesis are ill-defined. Here, we investigated the role of the second messenger nicotinic acid adenine dinucleotide phosphate (NAADP), which is known in several cell types to induce Ca2+ oscillations that initiate from acidic stores such as lysosomes, likely via two-pore channels (TPCs, TPC1 and 2). Methods and results An NAADP antagonist called Ned-K was developed by rational design based on a previously existing scaffold. Ned-K suppressed Ca2+ oscillations and dramatically protected cardiomyocytes from cell death in vitro after ischaemia and reoxygenation, preventing opening of the mitochondrial permeability transition pore. Ned-K profoundly decreased infarct size in mice in vivo. Transgenic mice lacking the endo-lysosomal TPC1 were also protected from injury. Conclusion NAADP signalling plays a major role in reperfusion-induced cell death and represents a potent pathway for protection against reperfusion injury

DESIGN AND EVALUATION OF TRANSDERMAL FILMS CONTAINING KETOPROFEN FOR THE TREATMENT OF ARTHRITIS

Transdermal drug delivery systems of ketoprofen have been formulated using solvent casting method. Monolithic and membrane controlled systems were prepared by using Hydroxy propyl methyl cellulose (HPMC)  and ethyl cellulose (EC)  polymers by incorporation glycerin and dibutyl phthalate as plasticizers respectively. Drug polymer interactions were studied by FT-IR and differential scanning calorimetry which showed no interaction between drug and the polymers used. All the patches were uniform with respect to physico-chemical evaluation. The drug uniformity in the patch was observed by scanning electron microscopy which showed uniform distribution of drug throughout the patch. The in vitro drug release studies indicated that HPMC and EC patches with no permeation enhancer released the drug in an optimum rate. The various permeation parameters such as flux, permeability co-efficient, enhancement ration and diffusion rate constants were determined for all the formulations. The effect of permeation enhancer on the diffusion of ketoprofen across rat skin was determined. The maximum flux of 9.52x10-2 mg/cm2/hr was observed with HPMC monolithic system containing 5% v/v d-limonene. A significant improvement of flux was observed with 5% d-limonene than that of 20% oleic acid. The in vitro release studies revealed that the release was sustained upto 24 hr and followed zero order kinetics. Keywords: Ketoprofen, HPMC, EC, d-limonene and Oleic acid

Studies on High Density Planting and Nutrient Requirement of Banana in Different States of India

An experiment was conducted under the ICAR-All India Coordinated Research Project on Fruits to study the high-density planting (HDP) and nutrient requirement of banana at six research centres across the country, including Bhubaneswar (Orissa), Gandevi (Gujarat), Jalgaon (Maharashtra), Jorhat (Assam), Kannara (Kerala) and Mohanpur (West Bengal) to enable higher productivity of banana and profit to farmers. The objective of this study was to explore the possibility of increasing productivity through the intervention of only per unit plant population (through planting system) and level of nutrition, but without any interference to the regional choices of variety (eg., choice variety Nendran for Kerala or Martaman for West Bengal), production system (mono/poly- clone, single/multi-year plantation, and POP of respective states), for which national productivity ranges are much skewed also. Results indicated that intervention of only plant density could increase the productivity of banana within the existing system of production and choice of a variety of different regions or states. The experiment was laid out in RBD with four planting densities (S1P2, S1P3, S2P2 and S2P3, where S1=2m x 3m, S2=1.8m x 3.6m, P2=2 suckers/hill, P3=3 suckers/hill), three nutrition levels (F1, F2 and F3 , which is 100%, 75% and 50% of RDF) and one with region-specific conventional planting density and nutrition (100% of RDF) practices as control. The results of this experiment showed that HDP (S1P3, 5000 plants /ha) in banana, accommodating three suckers per hill at 2m x 3m spacing increased productivity over the conventional system at the Bhubaneswar, Gandevi, Jorhat, Kannara and Mohanpur centres. The increase in productivity due to HDP (5,000/ha) over control was 28.9% (RDF 25%) to 50.6% (RDF 100%) at Bhubaneswar, 15.2% (RDF 25%) to 21.9% (RDF 100%) at Gandevi, 4.0% (RDF 25%) to 7.4% (RDF 100%) at Jorhat, 33.5% (RDF 25%) to 43.5% (RDF 100%) at Kannara and 46.5% (RDF 25%) to 79.0% (RDF 100%) at Mohanpur centre. The nutrient requirement under HDP was 100% RDF at Kannara, 75% RDF at Bhubaneswar and Mohanpur and 50% RDF at Gandevi and Jorhat centres, which indicates a saving in cost of fertilizer input by 25% -50%. It is, therefore, recommended for HDP (5000 plants/ha) in banana, accommodating three suckers per hill at 2m x 3m (6.6 ft x 3.8 ft) spacing with 50% RDF in the agro-climatic regions of Gandevi and Jorhat, with 75% RDF in the agro-climatic regions of Bhubaneswar and Mohanpur and with 100% RDF in the agro-climatic region of Kannara in order to ensure higher productivity and profit to farmers

Current exposure of Italian women of reproductive age to PFOS and PFOA: a human biomonitoring study

Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) concentrations were determined in serum samples collected in 2011-2012 from 549 nulliparous Italian women of reproductive age who resided in six different Italian Regions. Assessment of exposure to perfluorinated compounds was part of a large human biomonitoring study (Project Life Plus "Womenbiopop") that aimed at examining the exposure of women of reproductive age to priority organic pollutants. The median concentrations of PFOS and PFOA were 2.43, and 1.55ngg-1, respectively. Significant differences in the concentrations of both compounds were observed among the six Regions. Women from central Italy had the highest levels of both compounds, followed by women from northern Italy, and southern Italy. No differences in the PFOS concentrations were found between women from urban/industrial areas and women from rural areas, whereas the levels of PFOA were significantly higher in women residing in urban/industrial areas than in women residing in rural areas. Taken together, the observed concentrations confirm that the overall exposure of the Italian population is among the lowest observed in industrialized countries. A downward temporal trend in exposure was observed for both compounds when comparing the results from the present study with those assessed in a study conducted in 2008

Regulation and drug modulation of a voltage-gated sodium channel: Pivotal role of the S4–S5 linker in activation and slow inactivation

Voltage-gated sodium (NaV) channels control excitable cell functions. While structural investigations have revealed conformation details of different functional states, the mechanisms of both activation and slow inactivation remain unclear. Here, we identify residue T140 in the S4–S5 linker of the bacterial voltage-gated sodium channel NaChBac as critical for channel activation and drug effects on inactivation. Mutations at T140 either attenuate activation or render the channel nonfunctional. Propofol, a clinical anesthetic known to inhibit NaChBac by promoting slow inactivation, binds to a pocket between the S4–S5 linker and S6 helix in a conformation-dependent manner. Using 19F-NMR to quantify site-specific binding by saturation transfer differences (STDs), we found strong STDs in inactivated, but not activated, NaChBac. Molecular dynamics simulations show a highly dynamic pocket in the activated conformation, limiting STD buildup. In contrast, drug binding to this pocket promotes and stabilizes the inactivated states. Our results provide direct experimental evidence showing distinctly different associations between the S4–S5 linker and S6 helix in activated and inactivated states. Specifically, an exchange occurs between interaction partners T140 and N234 of the same subunit in activation, and T140 and N225 of the domain-swapped subunit in slow inactivation. The drug action on slow inactivation of prokaryotic NaV channels seems to have a mechanism similar to the recently proposed “door-wedge” action of the isoleucine-phenylalanine-methionine (IFM) motif on the fast inactivation of eukaryotic NaV channels. Elucidating this gating mechanism points to a possible direction for conformation-dependent drug development